Life cycle inventory(LCI), Life cycle impact assessment and finally Interpretation. Goal and scope, is the start of an explicit statement and this will set up the scope of the product. Life cycle inventory is an analysis with the goal of listing the products to and from the nature. The outcome of this is typically a flow chart. Life cycle impact assessment, is pretty straight forward given the name. This is aimed to evaluating the potential environmental impact each step from the LCI flow chart.…
choice. PROTOCOL: CTAB method for DNA extraction protocol Take 2ml broth in a micro centrifuge tube Centrifuge @14,000 rpm for 15 minutes Discard the supernatant and collect the pellet Dissolve the solution in GTE solution First vortex it and then centrifuge @8000rpm for 15 minutes Collect the pellet and add 450 µl of GTE solution plus add 50µl Lysosymes (freshly prepared) Incubate it and keep it overnight at 37 degrees Add 10% of 200 µl SDS and mix gently Add 50µl of…
X-ray Spectroscopy (EDX) analysis was done by using the EDX system supplied with the FESEM. The bulk density (ρB) of the composite was determined using the relation: ρ_B=m/(〖πr〗^2 t) where m is the mass, r is the radius and t is the thickness of the pellet. The X-ray density of the composite is given by the formula, ρ_x (composite)=(M_1+M_2)/(V_1+V_2 ) where M_1 is (1-x) times molecular weight of BFO and M_2 is x times molecular weight of LNMFO,〖 V〗_1=M_1/ρ_x (ferroelectric) and V_2=M_2/ρ_x…
Ricin is very poison which and found within a castor of beans, which are found naturally. Ricin is usually made by processing the left over castor beans. It ends up being in the form of powder, but can also be a mist, pellets, or can even be dissolved in water. If castor beans are ingested by chewing or swallowing them, the ricin will be released and cause injury. This substance is a very stable substance under average condition, but ricin can be inactivated when the conditions react a…
as Skinner's Box - to prove his theory. He placed a rat that had been deprived of food into an enclosed box. The rat is moving around the cage when it accidentally presses the bar; as a result a food pellet falls. The operant is the behavior just prior to the reinforcer which is the food pellet. In a relatively short period of time…
involuntary manslaughter is because he shot a pellet gun at the person, a gun not usually intentional to killing someone; this gun killed someone. With this gun killing someone it was found that no reasonable person would have found that killing someone with this gun was unforeseeable. This also contributes to this case because of the fact that running across the street at two am, and having a car hit Officer Bagger is not as unforeseeable as a pellet gun being able to kill someone. If Nunn was…
(+) labeled microtube by using a clean pipette. Afterwards, the (+) microtube was placed in the centrifuge (max speed) for five minutes followed by sheeting down the liquid supernatant above the pellet. Then, 250 µl of TE solution was added to each tube followed by adding 1 drop of lysozyme to the pellet containing the bacteria [9]. Bacterial Lysis (Purification…
Methods Each trial consisted of isolating the hearts of two mice, aged anywhere between 8-15 weeks, with the strain FVB/NJ of the Mus genus. Eight trials were conducted throughout the duration of the experiment. No female mice were used for any of the trials because because the research in the lab concentrates on males, due to known differences in protein expression among the two sexes. The effect of the VU591 drug on mitochondrial KATP function in wildtype mice was measured by calculating the…
occur indoors with as few people as possible to reduce stress on the animal. A bag, box, or towel wrap may be used when weighing the bird. The feathers of a barn owl may hide a malnourished individual. To diagnose malnourishment, pests, or disease, pellets may be examined or a fecal float may be requested. (Sieders,…
cellular components while maintaining the enzyme activity within each of the four fractions. The first step in fractionation is commonly centrifugation as it separates the components based on size, meaning that after the initial centrifugation, the pellet will contain whole cells, and the supernatant will contain the extracellular component (Alberts et al., 2002). The sample we used was spun down and the supernatant was removed, which presumably contained any secreted molecules within the…