The Nisin Bacteriocin Introduction Nisin is a bacteriosin peptide made up of 34 amino acid residues that contain uncommon amino acids such as lanthionine and didehydroalanine. It is a substance produced by both gram positive and gram negative bacteria to control the growth of other bacteria species to reduce competition for substrate and space. Nisin has received attention from researchers making it the most exploited bacteriosin (Shetty, Pometto and Levin, 2006). The attention from…
way. In the same manner, all the knowledge I learned from Microbiology class and laboratory sessions regarding microbial world equipped me to identify bacterial unknowns from a clinical sample. The task was to successfully separate and accurately identify two bacteria of a specimen taken from an abscess to safely and effectively treat the patient. A clinical sample # 76 was handed over by the professor with a microbiology requisition. Requisition stated that the source of the…
To determine the unknown’s morphology is the first step in narrowing down possible organisms. A MacConkey and Mannitol Salt Agar tests were conducted on the organism and revealed the following. No growth on the MacConkey plate resulting in a gram positive bacteria and the Mannitol Salt Agar result showed it could be Staphylococcus or Micrococcus. (1) A gram stain test was used in determining the shape, size and arrangement of the unknown bacteria (1). The unknown organism is heat fixed onto the…
‘Revenge of the microbes’ by Abigail A. Salyer and co-author Dixie D. Whitt are both professors in the department of Microbiology at the University of Illinois. After thorough research by the professors, this great piece was published. This masterpiece, happens to be a reliable source for mere educative information about the microbiological world we find ourselves in but it could also be used as a good source for rich information about the activities of microbes and the role they play in our…
clearing would appear around the disk containing the antibiotic. The diameter of this circle was then measured and compared with a table found on page 95 of the Microbiology Lab Manual to understand the antibiotics resistance, intermediate and sensitive levels, as they differ for each antibiotic. The numbers on the table in the Microbiology Lab Manual represented whether the microbe was resistant, shown in the light blue color on the graph, intermediate, shown in purple, or sensitive to the…
I walk down a narrow hallway into the microbiology laboratory on my college campus. I am met outside of the door by the professor. He proceeds to set the guidelines for proper safety in the lab. The number one thing he says and repeats is the concept of asepsis or the absence of microorganisms. After I finally get the okay to walk through the door, ditching my things there as I continue into the lab. Sanitization of your hands and the lab bench are the priority, immediately followed by placing…
Bacterial community of some waste dumpsites in Lagos, Nigeria Moro, D.D.1, Bello, H.A.2 and Akano, S.O.3 1. Department of Microbiology, Lagos State University, Ojo, Lagos State, Nigeria. 2. Department of Science Technology,Gateway Polytechnic, Igbesa, Ogun State, Nigeria. 3. Department of Medical Microbiology, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria. ABSTRACT Bacteriological…
pathogens, Salmonella enterica serovar Typhi.is known as Salmonella Typhi and students could confuse them with any other of the many many forms of Salmonella enterica at first glance. We know this bacterium as the cause of Typhoid. In the world of microbiology, it is known to be gram negative, it does not hold the violet dye in testing. It is both aerobic and anaerobic. In other words, it is a facultative anaerobe. It belongs to the genus Salmonella and the family Enterobacteriaceae and…
1. The biggest potential source of contamination of the locations we tested in the Distribution of Microorganisms would have to be the floor of the lab. The reason why this is the biggest potential source of contamination is because multiple people are coming in everyday from the outside world, bringing in microorganisms from the outside into the lab. And although we sanitize the counter and all the spaces that we work around, we don’t take precautions to necessarily clean the floor, making it…
Interchangeability of the two anthranilate synthase and evolutionary implications. Journal of Bacteriology, 172, pp.884–900. Fothergill, J.L. et al., 2007. Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain. BMC microbiology, 7, p.45. Fuse, K. et al., 2013. Reduction of virulence factor pyocyanin production in multidrug-resistant Pseudomonas aeruginosa. Journal of Infection and Chemotherapy, 19, pp.82–88. Hassan, H.M. & Fridovich, I., 1980. Mechanism of the…