Staining

it is the most important staining procedure in microbiology, it is used to distinguish between gram-positive and gram-negative organisms. The gram-positive cells stain purple, and the gram-negative stain a pinkish color. This staining is a differential stain that allows is used to identify the changes between organisms or differences within the same organism. Theory and Background: The Gram staining method was named after the Danish bacteriologist, Hans Christian Gram, in 1882. This method is now one of the most important staining procedures in microbiology. Steps of the Gram stain: Reagents: • Escherichia coli (slant culture) • Staphylococcus aureus (slant culture) • Crystal violet (primary stain) • Iodine solution (Gram’s iodine) • Decolorizer (ethanol) • Safranin (counterstain) • Water (in squirt bottle) Prepare a mixed smear using slant cultures: 1. Take two…

After my gram staining I was able to comfortably eliminate all of the gram negative results. This encouraged me in knowing that I was able to execute an experiment efficiently enough to eliminate some of my choices. I was also able to eliminate all of the streptococcus bacteria because my bacteria was in clusters and not in a chain arrangement. Gram staining also helped me in eliminating the bacilli because my interpretation of the observation was that my unknown bacteria was spherical in nature…

Gram staining was used to distinguish the unknown microbes between gram-positive microbes from gram-negative microbes. Gram-positive microbes contain a thick peptidoglycan with only one outer cell membrane, thus dying the microbes dark purple. Gram-negative microbes contain a thin peptidoglycan with an outer and inner cell membrane, dying the microbes pink when a gram stain test is done. Microbes A and C were gram- positive because of purple dye seen under the microscope. Microbe B was a…

INTRODUCTION There have been multiple staining techniques before the Gram stain came about in 1883. These old staining techniques brought about issues because they stained both the tissue and the cells so it was hard to differentiate between the two. Christian Gram is the inventor of the gram stain and found this technique when he was looking at Streptococcus pneumoniae in the lung tissue of people and animals (1). When working with these tissues, he found that this bacterium would take up a…

Introduction Gram staining is a method commonly used to determine the chemical make up of the cell wall of bacteria. The cell wall can stain either positive or negative, depending on its chemistry.The aim of this experiment. Gram staining was the first differential technique used and still in use to make microscopic vision of bacteria clearer while also sub-divides the bacteria into two major groups: Gram-positive and Gram –negative. This experiment should help us describe how Gram staining…

Gram Staining Procedure Doctors often want to determine what type of bacteria is present in a patient who has an infection. Aside from other tests, experts use gram staining procedure as one of the techniques to differentiate bacteria according to their cell wall characteristics. It involves using a dye to see which bacteria retain either color (violet or red) in their cell walls. The importance of using the gram staining procedure is related to the choice of treatment for the patient. Here is…

Introduction In this lab report I use two different techniques to identify Unknown A and Unknown B bacteria’s. These techniques are gram staining and metabolic testing. I first used Gram staining to distinguished and identify the bacteria’s. Han Christian discovered gram staining in 1882, he had biopsy a patient lung that had pneumonia. The main purpose for Gram staining is to identify if is Gram positive or Gram negative by using differential staining. While Gram staining shows the…

Gram staining is a technique where certain stains are used to identify bacteria. It was discovered in 1884 by Hans Christian Gram where he used stains to differentiate bacterial cells in diseased lung tissue. Gram staining has evolved over time to become a trusted and common diagnostic tool used in clinical and research settings. Gram stains are mainly used to identify unknown bacteria. It is often the first test used on an unknown sample of bacteria. For medicinal uses gram staining is commonly…

The staining of teeth has been an issue for a lot of the 20th century with more people caring how they dress and look a big factor is the way you smile and how your teeth look. People are always wondering why their teeth get darker. The bulk of the tooth consists of dentin. Dentin is a substance that is kind of like bone but it is harder. Covering the dentin is a tough durable layer named enamel. “ Enamel is composed primarily of calcium phosphate and is the hardest substance in the body.”…

Information about the Bacteria Pseudomonas aeruginosa During microbiology lab, an unknown bacteria culture in liquid broth was assigned to be identified by conducting a series of various tests. Nearly twenty different tests were conducted on the bacteria, but the most important of these was Gram staining test, gelatin stab test, and oxidase test. The results of these three tests allowed for the determination of the bacteria genus and species. The first test conducted was Gram staining. In the…