Lab Diagnosis Part 1 Page 1

Front 1

Give the laboratory diagnosis of viral infection

Back 1

1-5

Front 2

How do you collect in specimen collection

Back 2

Aseptically collection

Front 3

Delayed specimen temp

Back 3

1-6°C

Front 4

Delayed temp hour

Back 4

Less than 24 hrs

Front 5

Long storage temp

Back 5

-70°C

Front 6

Long temp sotrage:

Back 6

Freezer

Front 7

-70°C in long temp called

Back 7

Cryoprecipitate

Front 8

Refrain storage temp

Back 8

-20°C

Front 9

Wht refrain the temp bc

Back 9

It may cause formation of crytals that will inactivaye the virus

Front 10

swab Specimen collected by

Back 10

Dacron

Rayon

Cotton

Polyester

Front 11

Swab specimen examples!

Back 11

TENS

Front 12

Type of swab that is not allowed to to be used becayse it may cause damage or inactivate the virus

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Calcium alginate

Front 13

Specimen does not need transport mediym

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Blood

Csf

Urine

Bonemarrow

Pericardial

Pleural

Synoviql

Front 14

Vtm means

Back 14

Viral Transport medium

Front 15

If your specimen doesn't have vtm:

Back 15

Centri - 3000rpm

15-30 mins

Front 16

Specimen that needs VTM

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-specimen collected by swab

- Respiratory specimen

-Tissue Biopsy

Front 17

Examples of respiratory viruses

Back 17

IAFCPR

Front 18

In nasopharyngeal must use _____ swab insert _________ with ______

Back 18

Flexible

2-3cm

NSS

Front 19

Examples of respiratory specimen

Back 19

Throat

Nasopharyngeal

Front 20

VTM contains?

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Trypticasw Soy Broth, NSS

Front 21

VTM contain TSB and NSS

solution is

Back 21

Avoiding drying of the specimen

Front 22

Vtm also contain

Back 22

Gelatin, serum, albumin protein

Front 23

Vtm contains g.a.s.p

solution:

Back 23

To protectthe less viable viruses

Front 24

Vtm has ---- for inhibition of any growth

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Antibiotic and Antifungal

Front 25

Vtm has antibiotic and Adntifungal

solution

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For inhibition of any bacterial and fungal growth

Front 26

Methods of Viral isolation

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Cell culture

Animal inoculation

Embryonated egg inoculation

Front 27

In vitro is

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Cell culture

Front 28

In vivo is

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animal inoculation

and

Embryonated egg inoculation

Front 29

in cell culture if you dispense THE cell in tissue by----- using -----

Back 29

Grind or mince

Mortar and Pestle

Front 30

It is a Gold standard

and

presumptive test at the same time

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Cell culture

Front 31

Enumerate the categories of preparation of cell culture

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Primary cell culture

Finite cell culture

Continuous cell culture

Front 32

Deruved from an animal cell

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Primary cell culture

Front 33

In primary cell culture it has

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Rk and pMk

Front 34

A tissue is treated with enzyme to separate the cells what enzyme

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Trypsin

Front 35

Grow in monolayer across the glass

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Normal cells and Primary cells

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Do not grow in monolayer across the glass or plastic container

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Transformed cells and Continuous cell culture

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Cells are capable of

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cell division and splitting and fasting

Front 38

Transfer cell container to another to treat again with trypsin

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Finite cell culture

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50 generation

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Finte cell culture

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ex of finitecc

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HUman diploid fibroblasts

Human neonatal lung

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Heteroploid

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Normal and abnormal tissue

Front 42

Ex of continuous cell culture

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A549

HEP2

Front 43

what is a549

and HEP2

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Human lung carcinoma cell line

Human Neonatal lung

Front 44

Observation in cytophatic effect

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Change in size

Change in shape

Syncytia

Front 45

What is syncytia

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fusion of 2 or more cell

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Non culturable

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Hepatitis B virus

Front 47

culturable in tissue cells

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M. leprae

Front 48

HsV

Back 48

- +++ +++ +++ +++

Front 49

CMV

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- +++ - - -

Front 50

VZV

Back 50

- +++ - - +/-

Front 51

Enterovirus /mc

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+ + ++ - +

Front 52

RSV/ pauline

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-/+ + +++ - ++

Front 53

Influenza and Parainfluenza virus/ mama at papa

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+++ +/- - - -

Front 54

Adenovirus /lola at lolo

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+ ++ +++ ++ ++

Front 55

Large rounded cells

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Hsv

cmv

Front 56

Foci or rounded cells possible of syncytia

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VZV

Front 57

Refractile round cell in clusters

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Enterovirus

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Syncytia

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rsv

Front 59

Variiable no cp3

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iP

Front 60

Large rounded cell in clusters

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adenovirus