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45 Cards in this Set
- Front
- Back
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give the 3 procedures of microscopic identification directly in the specimen |
Light microscopy Untraviolet microscopy Electron microscopy |
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uses light microscopes |
light microscopy |
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presence of inclusion bodies |
light microscopy |
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possible cpe in |
light microscopy |
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ex of inclusion bodies in light microscopy |
Negri bodies/ Purkinje cells Guarnieri bodies Cowdry type a bodies |
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negri bodies purkinje cells virus |
rabies virus |
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rabies virus inclusion bodies |
negri bodies purkinje cells |
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Guarnieri bodies isolate in virus |
smallpox virus |
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smallpox virus inclusion bodies |
Guarnieri bodies |
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Viruses in Cowdry type a bodies |
hsv vzv |
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hsv vzv inclusion bodies |
cowdry type a bodies |
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cpe in light microscopy |
eosinophilic incluion surrounded by clear zone |
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a stained smear used in light microscopy |
tzanck test |
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tzacnk test a smear used to detect what |
vzv and hsv |
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what stain use in tszanck smear |
papanicolaou stain giemsa stain hematoxylin and eosin stain |
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Detects multinucleated giant cells and inclusions |
Tzanck test |
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alteration of the cell with hpv but have no cpe |
koilocytosis |
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other name of koilocytosis |
oval owls eye |
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squamous cells with enlarged nuclued surrounded bu non staining halo |
koilocytosis/ oval owls eye |
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squamous cells with enlarged nuclued surrounded bu non staining halo seen in what |
papanicolaou stain |
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koilocytosis indication of what viruses |
cmv hpv |
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uses immunofluoresence assay |
ultraviolet microcopy |
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what is the principle of ultraviolet microscopy |
uses immunofluorescense assay |
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clinical specimen of Adenovorus influenza Rsv Measles |
throat swab sputum bronchial lavage |
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if the specimen is throat swabsputumbronchial lavage who would be the viruses |
Adenovorus influenza RsvMeasles |
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cutaneous specimen is skin lesion and skin biopsy who would be the viruses |
human papilloma virus herpes simplex virus varicella zoster virus |
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human papilloma virusherpes simplex virusvaricella zoster virus what is the specimen |
skin lesion and skin biopsy |
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specimen is blood who is the virus |
cytomegalovirus |
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specimen of cytomegalovirus |
blood |
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directly seen structural arrangement of the virus |
electron microscopy |
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Visualization and detection in ______ and _____ in electron microscopy |
shape and size |
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for research purposes only |
electron microscopy |
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___ virus particle per ml |
1 million or 10^6 |
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magnification of virus in electron microscope |
50 000 - 60 000 |
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what viruses can isolate in feces |
rotavirus adenovirus norwalk virus |
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in vesicle fluid |
hsv vzv |
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in skin scraping |
molloscum contagiosum virus hpv |
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Problems with electron microcopy |
Expensive materials Expensive maintenance Required experienced observer Sensitivity often low |
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classical techniques in serological procedures |
complement fixation Hemagglutination inhibition test immunofluorescence technology neutralization test counterimmunoelectrophoresis |
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newer techniques in serological procedure |
Radioimmunoassay Enzyme linked immunosorbent assay Particle agglutination Western blot Recombinant immunoblot assay |
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Western blot detects |
Rna |
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Easterm blot detects |
DNA |
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Northern blot detects |
protein |
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use of antibody titer or specific immunoglobulon M (igM) |
Serological testing |
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produced 2-3 days after contacting virus |
antibody titer |
