Staining

In order to collect accurate data from an experiment, it is important to evaluate the environment that the lab will be taking pace. While my lab partners and I were doing this, we checked to see if the workspace is clean and clear of an unnecessary or harmful objects, especially food. Then our second step was to gather all the necessary supplies for the experiment. This included, a microscope, three glass microslides, alcohol, caffeine, and water, four pippets, then finally three water fleas.…

Organisms present on the Gram film were obtained from patient sputum. This suggests that the organisms can be present either in the airways or in the lungs. Gram film shows Gram-positive (purple- dark colour) elongated oval shaped (cocci) cells. Bacteria are present as diplococci (pairs of cocci), nevertheless they also appeared as single cells and short chains of few cells (four cells chain is visible at the Gram film). Morphological appearance of bacteria, Gram stain and presence of the…

The possibilities of two unknown microorganisms lead to phenylalanine deaminase test. The detection of phenylalanine deaminase enzyme was carried out by adding ferric chloride to phenyaline slant. When ferric chloride was added to phenylalanine deaminase tube, it turned into army green color( positive result) which indicated that unknown microorganism contains phenylalanine deaminase enzyme. On the other hand, no color change in the slant indicates negative result for this test. The choices…

Gram-negative. Another characteristic of identifying a Gram-positive or Gram-negative is to look at the shape. If it is negative, then the contour will be in rods. Motility: • Materials: Wash hand, Gloves, Unknown bacteria, Soft agar medium • The purpose of the motility test is to see if the bacteria is motile or not motile. • A positive outcome would be to see growth beyond the stab line. This would determine that the bacteria is motile and must have some sort of flagellum or cilia. •…

Once the unknown Gram-negative bacteria was isolated and stained, biological testing could proceed do that the bacteria could then be identified. The unknown bacterium was first used to test whether or not it was capable of oxidation or fermentation through the complex O-F Glucose medium test, and was found to use both oxidation and fermentation or fermentation only. The bacterium was then tested for the presence of citrate permease using a defined, Simmons Citrate agar, and was found to in fact…

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

1.Introduction: Background/Aim. 1.1 Nature of the microorganism: Enterococcus faecalis was placed under group D streptococci because of similar lysis characters; Identified as Gram positive, non-motile, anaerobic fermentative, lactic acid bacteria; are commensal inhabitant of intestinal bacteria and opportunistic pathogens. They occur singly or in pairs or chains. 1.2 Economic importance: Enterococcus faecalis is also used as a probiotic for production of Mediterranean cheese and meat products…

Introduction Termites, Isotera, are known to react to the exposure of the chemical substance found in ball point pens. Naturally, termites will follow any trail made up of pen ink. This specific observation was tested and carried out by Sim et al. They “investigated how termites select the direction of movement at an artificially-designed branching node, by making chemical trails on filter paper, drawing lines using a ballpoint pen which contained the chemical substance that induces the termite…

After receiving unknown organism #14, many tests were performed to discover the identity. After running a variety of tests, my results led me to believe that my unknown organism was Klebsiella pneumoniae. K. pneumonia produced a negative-rod gram stain. My organism, K. pneumoniae, tested positive for the three main carbohydrates, glucose, lactose, and sucrose. After running tests on these carbohydrates, a pH indicator color change from red to yellow signified a positive result, while remaining…

Unknown Identification Lab Report Elizabeth Munn BIOL 250L 008 April 1, 2015 Introduction For this lab report, the objective is to use different lab test to determine the unknown bacteria. According to the Bergey’s Manual of Determinative Bacteriology (1994), the morphology is straight rods and around .6-.10 micrometers wide and 1.2-3.0 micrometers long. It also is a gram-negative bacterium and opportunistic pathogens that causes different types of infections related to burns,…