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69 Cards in this Set
- Front
- Back
What stains is this? What does it stain?
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-Fontana Masson
-Melanin pigment in malignant melanoma |
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What stain is this? What does it stain?
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-Alcian Blue
-goblet cells |
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What structure is stained blue?
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-goblet cells
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What is the name of this stain? What is this stain used for?
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-Hematoxylin and Eosin (H and E)
-routine stain, nuclear detail |
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What is this stain? What does it stain?
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-Toluidine Blue
-Mast Cells |
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What is the name of this stain? What does it stain?
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-PAS
-carbohydrates, goblet cells |
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What are the bright magenta structures called? Why is it stained that way?
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-goblet cells
-glycol groups are converted to aldehydes by the periodic acid which allows them to take up the Schiff's reagent |
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What stain is this? What does it stain?
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-Masson Trichrome
-connective tissue fibers (elastic vs. collagenous) |
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What stain is this? What does it stain?
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-Verhoeff-Van Gieson
-differentiates collagenous and elastic tissue |
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What structures are represented by the different arrows?
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A: Muscle Cells
B: Elastic Fibers C: Collagenous Fibers |
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What stain is this? What does it stain?
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-Masson Verhoeff combination
-differentiates between collagenous and elastic fibers |
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What structures do the arrows point to?
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A: Muscle Tissue
B: Collagen fibers C: Elastic Fibers |
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What is the name of this stain? What does it stain?
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-Mallory's Connective Tissue Stain
-connective tissue |
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What is represented by the red portion? What is represented by the blue portion?
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-parenchymal tissue (secretory cells)
-CT stoma (supporting elements) |
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What is the name of this stain? What is it used for?
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-Golgi Stain
-nerve cells and fibers |
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What is the name of this stain? What does it stain? What are the name of the dark purple/black spots and what is their significance?
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-Cresyl Violet
-nervous tissue -Nissil Bodies are stained dark because they contain more RNA which takes up the dye more readily |
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What is the name of this stain? What does it stain?
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-Luxol Fast Blue
-myelin sheaths of axons and other nerve fibers |
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What is the name of this stain? What is it used to stain?
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-Luxol Fast Blue-Cresyl Violet combination
-nerve fibers with more distinct detail than plain LFB |
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What is the name for this stain? What does it stain?
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-Kluver-Barrera
-neurons |
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What are the names of the structures the arrows are pointing to?
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|
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What is the name of this stain? What does it stain for? What is represented by the black portion of the stain?
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-Fontana Masson
-melanin pigment -melanin pigment |
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What is the name of this stain? What is it used to stain?
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-Prussian Blue
-iron pigment |
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What is the name of this stain? What is this stain used for?
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-Toluidine Blue
-mast cells in tumors |
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What is the name of this stain? What does it stain?
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-Osmium Tetroxide
-Lipids (in light microscopes) |
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What is the name of the stain on the left? Right? What are both of these stains staining?
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-Oil Red O
-Sudan Black -lipids (frozen sections) |
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What is the name of this stain? What does it stain?
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-alcian blue
-mucins |
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What is responsible for the blue color in this stain?
What are the blue portions of this stain? |
-copper
-mucins and mucosubstances |
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What structures are stained pink?
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-cytoplasm
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What is the name of this stain? What does it stain?
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-alkaline phosphatase
-endothelial cells |
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What do the red portions of this stain signify?
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-red portions show areas of alkaline phosphatase activity
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What color will the nucleus stain in this stain?
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-blue
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What is the name of this stain? What does it stain?
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-Bielschowsky stain
-neurofibrillary tangles and senile plaques |
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What is represented by the portion stained black?
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-neurofibrillary tangles and senile plaques
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What is the name of this stain? What is it used to stain?
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-Cajal stain
-nervous tissue |
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What is the name of this stain? What does it stain for?
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-Congo Red
-amyloid |
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What color represents a positive reaction?
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-red
-the amyloid positive tissue stains red |
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What is the name of this stain? What does it stain for?
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-Cresyl violet
-neurons and glia (nissl substances) |
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What does this stain bind to?
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-acidic parts of the cells
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What is the name of this stain? What does it stain?
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-Fontana-Masson
-argentaffin granules and melanin |
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What is stained black? What is stained pink/red?
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-argentaffin granules and melanin
-nucleus |
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What type of stain is this? (what family of stains)
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-silver stain
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What is the name of this stain? What does it stain?
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-Giemsa stain
-peripheral blood smear and bone marrow |
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What is a secondary use for this stain?
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-parasites and malaria
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What reagent is responsible for the dark blue/purple color to this stain?
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-methylene blue azure
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What structures pick up the dark blue/purple color in this stain?
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-acidic structures
-cytoplasm, chromatin, platelets, leukocytes |
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What is the name of this stain? What will it stain?
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-Golgi stain
-neurons |
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What is the name of this stain?
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-Hematoxylin and Eosin
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What is the most common use for this stain?
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-routine histology
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What are the names of the dyes used in the stain? What does each stain?
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-Hematoxylin stains nucleic acids (nucleus)
-Eosin stains cytoplasm, connective tissue, muscle, colloid, red blood cells, and decalcified bone matrix |
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What is the name of this stain? What does it stain?
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-Luna stain
-elastin and mast cells |
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What is represented by the black portions of this stain? purple portion
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-nucleus
-elastin and mast cells |
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What is the name of this stain? What is stained?
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-Luxol fast blue
-myelin |
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What structures are stained blue? violet?
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-myelin
-neurons |
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What is the name of this stain? What does it stain?
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-Masson trichrome stain
-connective tissue |
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What are the three dyes that make up this stain?
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-light green, iron hematoxylin, acid fuchsin
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What is stained blue/green? red? pink? black?
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-collagen fibers
-muscle and keratin -cytoplasm -nucleus |
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What is the name of this stain? What does it stain?
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-Nissl stain
-nissl bodies in neurons (RNA and DNA) |
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What is stained purple/blue?
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-nissl substances
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What is the name for this stain? What does it stain
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-Oil Red O
-lipids |
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What is stained by the red portion? blue/black portion?
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-lipids
-nucleus |
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What is the name of this stain? What is it used to stain?
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-osmium tetroxide
-lipids |
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What is represented by the brown portion? black portion?
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-collagen and erythrocytes
-lipids and myelin |
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What is the name of this stain?
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-pap stain
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What is this stain used to stain?
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-gynecological smears
-sputum -urine -cerebrospinal fluid -abdominal fluid -pleural fluid -synovial fluid -semminal fluid -fine needle aspiration |
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What is the name of this stain? What does it stain?
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-Periodic acid-Schiff
-glycogen, mucin |
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What is stained magenta? blue? pink?
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-glycogen
-nuclei -cytoplasm |
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What is the name of this stain? What does it stain?
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-Prussian blue
-iron |
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What is the name of this stain? What is it used to stain?
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-PTAH (phosophotungstic acid hematoxylin)
-striated muscle fibers and mitochondria |
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What is represented by the blue portion? red?
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-muscle fibers and mitochondria
-collagen |