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Absorption Spectra of Haemoglobin species

According to Lambert-Beer's law, the contribution of each species at any given wavelength will be proportional to that species' concentration in the sample.

Absorption Spectra of Hb Species

Define a continuous monitor.

Asa defines this as no interruption of a simple measurement.

Chromophores?

Light absorbing molecule.

Isobestic point?

The Wavelength at which 2 or more molecules will have the same absorption.


In NIRS, it is used to calculate the total tissue hemoglobin concentration


Hb-HbO2 = 805nm in the near infrared Spectrum.


Absorption & Wavelength both equal

Near infrared wavelengths?

650 to 940nm.

Spatial resolution?

Depth of tissue being investigated is proportional to the distance between the light source and the detectors

Reflectancy Oximetry.?

The balance between oxygen supply and demand. It is thus a measure of the adequacy of tissue oxygenation.

Advantages of NIRS?

Safe


Easy


Wide range if clinical fields.

Disadvantages of NIRS

Lacks evidence based data


No clear guidance on how to intervene should a problem be detected.


NIRS monitors are a work in progress


List recommended clinical uses for NIRS

List factors reducing cerebral blood 4 flow & CaO2 4

Treatment Algorithm for regional cerebral desaturation during cardiopulmonary bypass.

Compare Beer's law to Lamberts law.

Combined beer-lambert law?

If a known intensity of light


Illuminates a chamber of a known dimension


Then the concentration of the dissolved substances can be measured if the intensity of the incident and transmitted light is measured.