Micro Lab Test 2

Front 1

mycelium

Back 1

collective growth of hyphae

Front 2

Saprotrough

Back 2

eat decaying matter

Front 3

Necrotrough

Back 3

eat dead matter

Front 4

biotrough

Back 4

eat living matter but don't kill it

Front 5

What do we grow fungi on?

Back 5

PDA - Potatoe dextrose Agar

Front 6

Antimicrobials

Back 6

reduce or kill microbes

Front 7

Physical methods to kill microbes

Back 7

high or low temp, pH, irradiation and fitration

Front 8

Disinfectants

Back 8

chemical compounds that are used on non-living objects (lysol, detergent and bleach)

Front 9

Antiseptics

Back 9

chemicals used on living tissue (hydrogen peroxide, rubbing alcohol, neosporin)

Front 10

Antimicrobials

Back 10

selectively kill or inhibit pathogens inside the host (antibiotics and synthetic antibiotics)

Front 11

disk diffusion method

Back 11

Kirby bauer

Front 12

What's measured in Kirby bauer?

Back 12

the zone of inhibition

Front 13

What agar is the kirby bauer test?

Back 13

Mueller-Hinton agar - which doesn't contain PABA that would throw off the test

Front 14

Name of the turbidity standard

Back 14

McFarland standard

Front 15

Blood Agar

Back 15

differential but not selective. 5% sheeps blood

Front 16

Catalase test

Back 16

tests to see if bac can convert H2O2 to H2O +O2.

Front 17

MSA

Back 17

S + D. mannitol fermenting shown by phenol red turning yellow. Salt env - growth on plate. Only grows gram+

Front 18

MacConkey

Back 18

S+D. Only grows gram -. Will grow all gram -, only lactose fermenters will turn the agar red (neutral red)

Front 19

Eosin Methylene Blue

Back 19

S+D. only grows gram -. lactose fermentors will turn black. Strong lactose fermenters will turn it metallic green black.

Front 20

Salmonella-Shigella

Back 20

S+D. Only grows salmonella and Shigella. Will show red for lactose fermenters. Will show black for lactose fermenters and H2S gas

Front 21

TSI slant

Back 21

uses phenol red. Tests for H2S production, produce gas from fermentation, and fermentation of glucose, lactose and sucrose. Slant/Butt/gas/H2S

Front 22

Positive for Caseinase

Back 22

B. subtilis

Front 23

Positive for starch hydrolysis

Back 23

Bacillus subtilis

Front 24

Positive for gelatinase

Back 24

Staphylococcus aureus

Front 25

EMB - metallic green results

Back 25

Escherica coli and Klebsiella pneumoniae

Front 26

EMB - Colorless colonies/coloring of medium

Back 26

Salmonella typhimurium and Yersinia enterocolica

Front 27

SS results

Back 27

Colorless colonies with black centers. E. coli and K. pneumoniae pink colonies. y. enterocolitca - colorless colonies.

Front 28

MacConkey results

Back 28

E. coli and K. pneumoniae - pink colonies. S. typhimurium and Y. enterocolitica - colorless colonies

Front 29

TSI slant results A/A/v/-

Back 29

E. coli and K. pneumoniae

Front 30

TSI slant results K/A/-/+

Back 30

S. typhimurium

Front 31

TSI slant results K/A/v/-

Back 31

Y. enterocolitica

Front 32

Positive for citrate

Back 32

S. typhimurium and K. pneumoniae

Front 33

Non hemolysis in Blood agar

Back 33

S. epidermis

Front 34

Alpha hemolysis in Blood agar

Back 34

Streptococcus pneumoniae

Front 35

Beta hemolysis in Blood agar

Back 35

Streptococcus pyogenes and Staphylococcus aureus

Front 36

Positive for Catalase

Back 36

Staphylococcus aureus and Staphylococcus epidermis

Front 37

What grows on MSA?

Back 37

Staphylococcus aureus - medium turns yellow. Staphylococcus epidermis - growth, no color change

Front 38

T4r only infects what bac?

Back 38

E. coli

Front 39

Dye used to prepare fungi slide

Back 39

Methylene Blue

Front 40

Phenol red is used in both ___ and ___ agars

Back 40

MSA and TSI

Front 41

Neutral red is used in both ___ and ___ agars

Back 41

MacConkey and SS

Front 42

What gives Alpha hemolysis, neg catalase and NaCl intolerant?

Back 42

Streptococcus pneumoniae

Front 43

What does a positive result for citrate test look like?

Back 43

blue

Front 44

Unknown 1, 2, 3

Back 44

Y. enterocolitica, S. pyogenes, S. aureus

Front 45

mycelium

Back 45

collective growth of hyphae

Front 46

Saprotrough

Back 46

eat decaying matter

Front 47

Necrotrough

Back 47

eat dead matter

Front 48

biotrough

Back 48

eat living matter but don't kill it

Front 49

What do we grow fungi on?

Back 49

PDA - Potatoe dextrose Agar

Front 50

Antimicrobials

Back 50

reduce or kill microbes

Front 51

Physical methods to kill microbes

Back 51

high or low temp, pH, irradiation and fitration

Front 52

Disinfectants

Back 52

chemical compounds that are used on non-living objects (lysol, detergent and bleach)

Front 53

Antiseptics

Back 53

chemicals used on living tissue (hydrogen peroxide, rubbing alcohol, neosporin)

Front 54

Antimicrobials

Back 54

selectively kill or inhibit pathogens inside the host (antibiotics and synthetic antibiotics)

Front 55

disk diffusion method

Back 55

Kirby bauer

Front 56

What's measured in Kirby bauer?

Back 56

the zone of inhibition

Front 57

What agar is the kirby bauer test?

Back 57

Mueller-Hinton agar - which doesn't contain PABA that would throw off the test

Front 58

Name of the turbidity standard

Back 58

McFarland standard

Front 59

Blood Agar

Back 59

differential but not selective. 5% sheeps blood

Front 60

Catalase test

Back 60

tests to see if bac can convert H2O2 to H2O +O2.

Front 61

MSA

Back 61

S + D. mannitol fermenting shown by phenol red turning yellow. Salt env - growth on plate. Only grows gram+

Front 62

MacConkey

Back 62

S+D. Only grows gram -. Will grow all gram -, only lactose fermenters will turn the agar red (neutral red)

Front 63

Eosin Methylene Blue

Back 63

S+D. only grows gram -. lactose fermentors will turn black. Strong lactose fermenters will turn it metallic green black.

Front 64

Salmonella-Shigella

Back 64

S+D. Only grows salmonella and Shigella. Will show red for lactose fermenters. Will show black for lactose fermenters and H2S gas

Front 65

TSI slant

Back 65

uses phenol red. Tests for H2S production, produce gas from fermentation, and fermentation of glucose, lactose and sucrose. Slant/Butt/gas/H2S

Front 66

Positive for Caseinase

Back 66

B. subtilis

Front 67

Positive for starch hydrolysis

Back 67

Bacillus subtilis

Front 68

Positive for gelatinase

Back 68

Staphylococcus aureus

Front 69

EMB - metallic green results

Back 69

Escherica coli and Klebsiella pneumoniae

Front 70

EMB - Colorless colonies/coloring of medium

Back 70

Salmonella typhimurium and Yersinia enterocolica

Front 71

SS results

Back 71

Colorless colonies with black centers. E. coli and K. pneumoniae pink colonies. y. enterocolitca - colorless colonies.

Front 72

MacConkey results

Back 72

E. coli and K. pneumoniae - pink colonies. S. typhimurium and Y. enterocolitica - colorless colonies

Front 73

TSI slant results A/A/v/-

Back 73

E. coli and K. pneumoniae

Front 74

TSI slant results K/A/-/+

Back 74

S. typhimurium

Front 75

TSI slant results K/A/v/-

Back 75

Y. enterocolitica

Front 76

Positive for citrate

Back 76

S. typhimurium and K. pneumoniae

Front 77

Non hemolysis in Blood agar

Back 77

S. epidermis

Front 78

Alpha hemolysis in Blood agar

Back 78

Streptococcus pneumoniae

Front 79

Beta hemolysis in Blood agar

Back 79

Streptococcus pyogenes and Staphylococcus aureus

Front 80

Positive for Catalase

Back 80

Staphylococcus aureus and Staphylococcus epidermis

Front 81

What grows on MSA?

Back 81

Staphylococcus aureus - medium turns yellow. Staphylococcus epidermis - growth, no color change

Front 82

T4r only infects what bac?

Back 82

E. coli

Front 83

Dye used to prepare fungi slide

Back 83

Methylene Blue

Front 84

Phenol red is used in both ___ and ___ agars

Back 84

MSA and TSI

Front 85

Neutral red is used in both ___ and ___ agars

Back 85

MacConkey and SS

Front 86

What gives Alpha hemolysis, neg catalase and NaCl intolerant?

Back 86

Streptococcus pneumoniae

Front 87

What does a positive result for citrate test look like?

Back 87

blue

Front 88

Unknown 1, 2, 3

Back 88

Y. enterocolitica, S. pyogenes, S. aureus