Mcb2010L Final Exam

Front 1

Facultative Anaerobe?

Back 1

Does NOT require oxygen.
Can grow with or without it
Able to DETOXIFY toxic by products of oxygen.

Front 2

In a Facultative anaerobe test-tube, what would you expect to see?

Back 2

Growth THROUGHOUT the test tube.
Due to ability to grow with or without oxygen.

Front 3

Microaerophile

Back 3

Does NOT grow in atmospheric oxygen, but REQUIRES a SMALL AMT for growth.
Able to detoxify toxic by products of oxygen.

Front 4

For a microaerophile, what would you expect to see in a test-tube?

Back 4

Growth in the MIDDLE of tube. Due to not being able to grow in high oxygen, or no oxygen.

Front 5

An example of a Microaerophile.

Back 5

Micrococcus luteus.

Front 6

An example of a Facultative Anaerobe.

Back 6

E. Coli

Front 7

Anaerobe?

Back 7

Lacks the enzyme for using oxygen. Can NOT live in oxygen. NOT able to detoxify toxic by products of oxygen.

Front 8

Regarding Anaerobes, what would you expect to see in a test tube?

Back 8

Growth at BOTTOM of tube.
Due to not being able to grow in oxygen.

Front 9

An example of an Anaerobe.

Back 9

Clostridium.

Front 10

What does it mean for test tubes have an oxygen gradient?

Back 10

The highest oxygen level at the top of the tube, and little to no oxygen at the bottom.

Front 11

Anaerobic Jar ??

Back 11

A jar with a chemical pack and water is used, producing hydrogen and carbon dioxide. The hydrogen reacts with the free oxygen in the jar producing water, and an ANAEROBIC condition. A methylene blue indicator is used to show the oxygen content. Blue - oxygen. white - No oxygen.

Front 12

In an anaerobic jar, what does the chemical pack and water produce in the jar?

Back 12

Hydrogen and Carbon dioxide.

Front 13

In an anaerobic jar, what kind of indicator is used to show the oxygen content?

Back 13

Methylene blue

Front 14

In an anaerobic jar, what do the resultant colors indicate?

Back 14

Blue = oxygen
White = oxygen

Front 15

Microbial death means....

Back 15

Permanent loss of reproductive capability, even under optimal growth conditions.

Front 16

What is EMB Agar?

Back 16

Eosin Methylene Blue Agar
Used for isolation of fecal coliforms

Front 17

What kind of bacteria grow on EMB agar?

Back 17

Gram negative

Front 18

What 2 bacteria types could grow on EMB?

Back 18

E. Coli
Enterobacter

Front 19

How can you tell the difference btwn
E. Coli and Enterobacter on EMB agar?

Back 19

E. coli grows green metallic b/c high lactose fermentation.
Enterobacter -- pink color b/c of low lactose fermentation.

Front 20

E. Coli + Hektoen agar = ??

Back 20

Has a pink-orange color

Front 21

Enterobacter + Hektoen agar = ??

Back 21

Pink-orange color

Front 22

Why won't Gram + bacteria grow on EMB agar?

Back 22

Due to bile salts and some dyes on these plates.

Front 23

What other bacteria have no color on the EMB Agar?

Back 23

Proteus
Salmonella
Shigella

Front 24

Why don't Proteus, Salmonella, and Shigella grow on EMB?

Back 24

Lactose non-fermenter

Front 25

Out of proteus, salmonella, and shigella, which are true pathogens and which are opportunistic?

Back 25

True pathogens - Salmonella, Shigella
Opportunistic - Proteus

Front 26

Proteus + Hektoen = ?

Back 26

Black precipitate on Hektoen agar
b/c of H2S production

Front 27

Salmonella + Hektoen = ?

Back 27

Black precipitate on Hektoen agar b/c of H2S production

Front 28

Shigella + Hektoen = ?

Back 28

Blue-green color
No Black precipitate b/c of NO H2S production

Front 29

What bacteria are not lactose fermenters?

Back 29

Proteus, Salmonella, Shigella

Front 30

Mannitol Salt Agar

Back 30

Selective media
Distinguishes btwn pathogenic vs. nonpathogenic staphylococcus.

Front 31

If MSA turns yellow, then....

Back 31

this is S. AUREUS

Front 32

If MSA turns pink, then....

Back 32

this is S. epidermitis.

Front 33

What test confirms and gives quick results of the presence of S. aureus?

Back 33

Slide agglutination test

Front 34

What's the pH of the MSA when its S. aureus?

Back 34

<6.8

Front 35

Staphylococcus aureus.

Back 35

Mannitol fermenter
Bubbles in H2O2

Front 36

Stahphylococcus epidermitis

Back 36

Does not ferment mannitol.
Pink color
pH > 7.4

Front 37

Staph Aureus and Staph epidermidis bubble with?

Back 37

H2O2

Front 38

Hemolysis: S. aureus?

Back 38

Beta hemolysis

Front 39

Hemolysis: S. epidermidis?

Back 39

Gamma hemolysis (None)

Front 40

Hemolysis types

Back 40

Beta (Complete)
Alpha (partial)
Gamma (None)

Front 41

What distinguishes Staph from Strep?

Back 41

Catalase test
- Staph will cause bubbling with H202
- Strep will not bubble with H2O2

Front 42

Will Gram (-) grow on Mannitol ?

Back 42

No. Because of sodium chloride of 7.5%

Front 43

Hemolysis: Streptococcus Pyogenes?

Back 43

Beta hemolysis

Front 44

Hemolysis: Strep pneumoniae?

Back 44

Alpha (partial)
Greenish color
Sensitive to Optichin (P-disc)

Front 45

Other than catalase testing, how can you differentiate btwn S. Aureus and Strep pyogenes?

Back 45

Strep pyogenes is sensitive to A disc.
S. aureus is not.

Front 46

How do you perform urine collection?

Back 46

Clean Catch.
Clean genitals with wipe.
Second flow of urine.

Front 47

2 Blood agars: 1 is sensitive to Bacitracin (A disc) and the other is sensitive to Optichin (P disc). What are these microorgs?

Back 47

The first -- Strep Pyogenes
The 2nd -- Strep pneumoniae

Front 48

2 Blood agars: 1 causes Beta hemolysis. The other causes Alpha hemolysis (has green color). What are these?

Back 48

The first -- Strep. Pyogenes
The 2nd -- Strep pneumoniae

Front 49

What 2 types of growth on SAB?

Back 49

Good growth (White, creamy)
Pinpoint colones

Front 50

Other than catalase testing, how can you differentiate btwn S. Aureus and Strep pyogenes?

Back 50

Strep pyogenes is sensitive to A disc.
S. aureus is not.

Front 51

How do you perform urine collection?

Back 51

Clean Catch.
Clean genitals with wipe.
Second flow of urine.

Front 52

2 Blood agars: 1 is sensitive to Bacitracin (A disc) and the other is sensitive to Optichin (P disc). What are these microorgs?

Back 52

The first -- Strep Pyogenes
The 2nd -- Strep pneumoniae

Front 53

2 Blood agars: 1 causes Beta hemolysis. The other causes Alpha hemolysis (has green color). What are these?

Back 53

The first -- Strep. Pyogenes
The 2nd -- Strep pneumoniae

Front 54

What 2 types of growth on SAB?

Back 54

Good growth (White, creamy)
Pinpoint colones

Front 55

On SAB: Good, creamy/white growth means it could be?

Back 55

Candida

Front 56

On SAB: Pinpoint colonies mean it could be ?

Back 56

E. Coli
Enterobacter
Staph. aureus
Staph. epidermidis

Front 57

HIV: What are some human secretions?

Back 57

Semen
Vaginal secretions
Breast milk

Front 58

HIV: Medication?

Back 58

AzT

Front 59

ELISA Test?

Back 59

Determines if you have HIV antibodies
Enzyme Linked immunoabsorbent assay
Always run a 2nd ELISA Test if (+)

Front 60

Risk factors that increase HIV likelihood?

Back 60

Multiple partners
Sharing needles

Front 61

For EMB, Blood agar, and SAB: Describe S. aureus' appearance.

Back 61

EMB - No growth. (g +)
BA - Beta hemolytic (see thru it)
SAB -- Pinpoint colonies

Front 62

For EMB, Blood agar, and SAB:
Describe S. epidermidis' appearance

Back 62

EMB --- No growth (but could if it is mixed)
BA -- Gamma hemolytic (None)
SAB -- Pinpoint (or yeasty looking if mixed)

Front 63

For EMB, Blood agar, and SAB:
Describe S. pyogenes' appearance.

Back 63

EMB -- Doesn't grow.
BA -- Beta hemolysis (Complete)
SAB -- No growth

Front 64

How could I distinguish Strep from Staph in looking at 3 unknown agars (BA, EMB, SAB)?

Back 64

Both are Beta.
Both are g + --> no growth on EMB.
But ONLY S. aureus grows on SAB.
S. pyogenes DOES NOT grow on SAB.

Front 65

What is this?

Back 65

Anaerobic Jar

Front 66

Describe the oxygen relation in these test tubes.

Back 66

1 - Anaerobe
2 - Microaerophile
3 - Facultative anaerobe