What does oxidase positive even mean?

OX+ normally means that the bacterium contains cytochrome c oxidase and can therefore utilize oxygen for energy production with an electron transfer chain.

LF oxidase negative TSI A(K)/A H2S Produced Indole negative most of the time

Citrobacter freundii Citrobacter is a genus of Gram-negative coliform bacteria in the Enterobacteriaceae family.

NLF oxidase negative TSI K/A H2S production No indole formation Swarming colonies

Proteus mirabilis Proteus mirabilis is a Gram-negative, facultatively anaerobic bacterium. It shows swarming motility, and urease activity. P. mirabilis causes 90% of all 'Proteus' infections in humans.

NLF oxidase negative TSI A(K)/A (NLF) H2S Production Indole Positive Swarming colonies

Proteus vulgaris Proteus vulgaris is a rod-shaped, Gram negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water and fecal matter. It is grouped with the enterobacteriaceae and is an opportunistic pathogen of humans. It is known to cause urinary tract infections and wound infections.

NLF oxidase negative A (K)/A no H2S few indole postiive Some have red/orange pigment

Serratia marcesens Serratia marcescens is a species of Gram-negative, rod-shaped bacterium in the family Enterobacteriaceae. A human pathogen, S. marcescens is involved in nosocomial infections, particularly catheter-associated bacteremia, urinary tract infections and wound infections,[1][2] and is responsible for 1.4% of nosocomial bacteremia cases in the United States.[3] It is commonly found in the respiratory and urinary tracts of hospitalized adults and in the gastrointestinal system of children.

NLF oxidase negative K/K No H2S Production Indole negative

Stenotrophomonas maltophilia S. maltophilia frequently colonizes breathing tubes such as endotracheal or tracheostomy tubes, the respiratory tract and indwelling urinary catheters.

Gram negative coccobacilli Small gram negative bacilli

suggestive of Hemophilus, fastidious and anaerobic organisms. Pseudomonas may occur as larger coccobacilli

Gram negative bacilli

Enteric gram negative rods * Enterobacteriaceae (=enteric gram-negative rods, enteric bacteria, coliform bacteria): a large, heterogeneous group of gram-negative rods * Natural habitat is the intestinal tract of humans and animals * Some enteric organisms (E.coli) are part of the normal flora and incidentally cause disease, while others (salmonellae, shigellae) are regularly pathogenic for human

Gram negative Aerobic bacilli/coccobacilli Do grow on MacConkey agar, ferment lactose

LF: Enterobacter E Coli Citrobacter Klebsiella

Gram negative Aerobic bacilli/coccobacilli Do grow on MacConkey agar, do NOT ferment lactose, do NOT ferment glucose

Oxidase positive: pseudomonas, burkholderia Oxidase negative: Acinetobacter Stenotrophomonas

Aerobic bacilli/coccobacilli Do grow on MacConkey agar, DO NOT ferment lactose, ferment glucose

Salmonella Shigella Proteus Providencia Mogranella others

Gram negative bacilli/coccobacilli Do NOT grow on MacConkey agar

Haemophilus Campylobacter/Legionella/Bordetella require special growth media

Gram negative intracellular diplococci

Probably neisseria or branhamella

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Lab Tests - Gram Negative

Lab Tests - Gram Negative

by pzadnik9, Sep. 2009

Subjects: bacteria id lab tests

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	Lactose Fermenter Oxidase negative TSI A/A (LF) No Hs2 production Indole positive	E. coli
	What does oxidase positive even mean?	OX+ normally means that the bacterium contains cytochrome c oxidase and can therefore utilize oxygen for energy production with an electron transfer chain.
	LF Oxidase negative TSI A/A (LF) No H2S Indole negative	Klebsiella pneumonia
	LF oxidase negative TSI A(K)/A H2S Produced Indole negative most of the time	Citrobacter freundii Citrobacter is a genus of Gram-negative coliform bacteria in the Enterobacteriaceae family.
	NLF oxidase negative TSI K/A H2S production No indole formation Swarming colonies	Proteus mirabilis Proteus mirabilis is a Gram-negative, facultatively anaerobic bacterium. It shows swarming motility, and urease activity. P. mirabilis causes 90% of all 'Proteus' infections in humans.
	NLF oxidase negative TSI A(K)/A (NLF) H2S Production Indole Positive Swarming colonies	Proteus vulgaris Proteus vulgaris is a rod-shaped, Gram negative bacterium that inhabits the intestinal tracts of humans and animals. It can be found in soil, water and fecal matter. It is grouped with the enterobacteriaceae and is an opportunistic pathogen of humans. It is known to cause urinary tract infections and wound infections.
	NLF Oxidase negative TSI K/A H2S production Few indole positive	Salmonella enteritis
	NLF oxidase negative A (K)/A no H2S few indole postiive Some have red/orange pigment	Serratia marcesens Serratia marcescens is a species of Gram-negative, rod-shaped bacterium in the family Enterobacteriaceae. A human pathogen, S. marcescens is involved in nosocomial infections, particularly catheter-associated bacteremia, urinary tract infections and wound infections,[1][2] and is responsible for 1.4% of nosocomial bacteremia cases in the United States.[3] It is commonly found in the respiratory and urinary tracts of hospitalized adults and in the gastrointestinal system of children.
	NLF oxidase positive (only one among the gram negative rods we studied in lab!) TSI K/K No H2S Indole negative Metallic/green sheen	Pseudomonas aeruginosa
	NLF oxidase negative K/K No H2S Production Indole negative	Stenotrophomonas maltophilia S. maltophilia frequently colonizes breathing tubes such as endotracheal or tracheostomy tubes, the respiratory tract and indwelling urinary catheters.
	Gram negative coccobacilli Small gram negative bacilli	suggestive of Hemophilus, fastidious and anaerobic organisms. Pseudomonas may occur as larger coccobacilli
	Gram negative bacilli	Enteric gram negative rods * Enterobacteriaceae (=enteric gram-negative rods, enteric bacteria, coliform bacteria): a large, heterogeneous group of gram-negative rods * Natural habitat is the intestinal tract of humans and animals * Some enteric organisms (E.coli) are part of the normal flora and incidentally cause disease, while others (salmonellae, shigellae) are regularly pathogenic for human
	Gram negative anaerobes	Bacteroides Fusobacterium
	Gram negative aerobic cocci	Neisseria
	Gram negative Aerobic bacilli/coccobacilli Do grow on MacConkey agar, ferment lactose	LF: Enterobacter E Coli Citrobacter Klebsiella
	Gram negative Aerobic bacilli/coccobacilli Do grow on MacConkey agar, do NOT ferment lactose, do NOT ferment glucose	Oxidase positive: pseudomonas, burkholderia Oxidase negative: Acinetobacter Stenotrophomonas
	Aerobic bacilli/coccobacilli Do grow on MacConkey agar, DO NOT ferment lactose, ferment glucose	Salmonella Shigella Proteus Providencia Mogranella others
	Gram negative bacilli/coccobacilli Do NOT grow on MacConkey agar	Haemophilus Campylobacter/Legionella/Bordetella require special growth media
	Gram negative cocci	Probable anaerobe
	Gram negative intracellular diplococci	Probably neisseria or branhamella

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