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60 Cards in this Set

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What does the plasmodesmata do?

Connects the cytoplasm of cells to each other

What does the cell wall do?


•Supports the plant cells


Give the cell shape•Supports the plant cells•Protect the contents of the cell from invading pathogens


•Supports the plant cells•Protect the contents of the cell from invading pathogens


•Protect the contents of the cell from invading pathogens


•Protect the contents of the cell from invading pathogens


What does the nucleus do?

•Controls the cell's activities


•Contains coded genetic instructions to make proteins

What does the lysosome do?

•Contains digestive enzymes/hydrolytic enzymes


•breaks down waste,old organelles,and pathogens


•Plays a role in apoptosis or cell death


What are ribosomes?

Where proteins are made

What does the rough endoplasmic reticulum do?

Synthesis and transport of proteins


•{ribosomes bound to the surface}

What does the Smooth endoplasmic reticulum do?

Synthesises and processes lipids and carbohydrates

What does the golgi apparatus do?

Modifies and packages proteins

What are calcium ions for?

•Nerve impulse transmission


•Muscle contraction

What are sodium ions for?

• Nerve impulse transmission


• Kidney function

What are potassium ions for?

• Nerve impulse transmission


• stomatal opening

What are hydrogen ions for?

• Catalysis of reactions


• pH determination

What are ammonium ions for?

• Production of nitrate ions by bacteria

How does the production of proteins occur?


1) proteins are made at ribosomes.


2) ribosomes on the ER get excreted, free ribosomes stay in cytoplasm.


3) New proteins produced in the ER are folded and processed.


4) Transported to the Golgi apparatus.


5) modified and packaged.


6) goes to vesicles to be transported around the cell/excreted.

What does the cytoskeleton do?

1) microtubules and microfilaments =support the cell's organelles and keeps them in position.



2) Strengthen the cell and maintain its shape.



3) movement of materials within the cells.



4) Can cause cell to move (flagella)

What stains can be used to stain samples for light microscopes?

Methylene blue


•eosin

What are carbohydrates made from?

Monosaccharides

What is the structure of alpha glucose?

What bonds join monosaccharides together?

Glycosidic bonds.

What 3 polysaccharides do I need to know?

• Starch


• Glycogen


• Cellulose

What makes up starch?

Amylose and Amylopectin

Amylose structure and function?

• Long, unbranched.


• Coiled = compact = good for storage.

Structure and function of amylopectin?

• Long, branched


• side branches allow the enzymes to break down = releases glucose quickly.

Structure and function of glycogen?

Excess glucose stored as glycogen.


• loads of branches = release glucose quickly.


• compact= storage

Structure and functions of cellulose?

• Long, unbranched chains of beta glucose.


• straight chains.


• linked together by hydrogen bonds = microfibrils= structural support.

What is the structure of triglycerides?

Held together by ester bonds

What does a saturated fatty acid mean?

No C=C bonds

What does an unsaturated fatty acid mean?

Contain C=C bond

What is the structure of phospholipids?

What is the structure and function of triglycerides?

• energy storage molecules.


• tails contain chemical energy


insoluble

Function of cholesterol?

Binds to the tails causing them to pack more closely together. This makes the membrane less fluid and more rigid

What bonds are formed when Amino acids join together?

peptide bonds

What is the structure of an amino acid?

• Variable group


• Amino group


• Carboxyl group

What is the primary structure of a protein?

Sequence of amino acids

What is the secondary structure of proteins?

Hydrogen binds form:


alpha helix/beta pleated

What is the tertiary structure of proteins?

Coiled further. More bonds. 3D.

What is the quaternary structure of proteins?

Several different polypeptides chains.

What bonds hold primary structure?

Peptide bonds

What bonds hold secondary structure?

Hydrogen bonds

What bonds hold tertiary structure?

• Disulphide


• Ionic


• Hydrogen


• hydrophyllic and hydrophobic

What are conjugated proteins?

Globular proteins with a non protein called a prosthetic group.

Examples of conjugate proteins?

Haemoglobin

What are fibrous proteins?

• Insoluble


• Strong

Example of fibrous proteins?

Keratin,collagen, elastin

What are the properties of keratin?

Hair, skin, nails


• Flexible


• hard and strong

What is the structure and function of globular proteins?

• Hydrophyllic on the outside


• soluble


• spherical

What are conjugated proteins?

Globular proteins with

What are the properties of collagen?

• Found in connective tissues


• 3 polypeptides


• strong


• flexible

Fibrous

What are the properties of elastin?

• Found in elastic connective tissues such as skin, blood vessels etc


• allows it to return to normal size after it has been stretched.

What are the properties of keratin?

• Hair, skin, nails


• flexibility determined by the amt of disulphide bonds

Explain how to test for reducing sugars?

Benedicts.


1) add benedicts (blue)


2) heat in water bath


3) reducing sugar= coloured precipitate


4) Red has a lot of reducing sugar

Explain how you would test for non reducing sugars?

If reducing sugar is negative...


1)get a new sample


2)Add HCL and heat it in water bath- 3)boil


4)neutralise it with sodium hydrogencarbonate


5) do benedicts again

What is the test for starch

Iodine


(Brown-blueblack)

What is the test for proteins?

Biuret


(Blue-purple)


1) add NaOH


2) add copper sulphate

What is the test for lipids?

Emulsion


1) add ethanol


2) if cloudy =positive

What does colorimetry do?

Determines conc of glucose.

How do u calibrate a colorimeter?

Using distilled water

What are biosensers used for?

A device that uses a biological molecule such as an enzyme to detect a chemical.


•The molecule produces a signal ->electric signal by a transducer


What things should u remember to do In a chromatography experiment?

Draw in pencil


Put lid


Fume cupboard (chemicals)


Hold paper or whatever with a clamp


Repeat,calc mean, exclude anomalies


How do u calculate R.F value?

Distance travelled by solvent÷ distance travelled by liquid.


Measure from the centre of the spot