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16 Cards in this Set
- Front
- Back
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What are proteins composed of?
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either a single polypeptide chain or a combination of two or more polypeptide chains
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What kinds of bonds secure the precise folding of a protein
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Hydrogen bonds, Ionic bonds, Van der Waals forces and hydrophobic attractions
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The bonds of proteins are senstive to what?
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to temperature and pH
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How is the active site pocket formed
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is formed as a direct result of the precise folding of the polypeptide chains. Thus the enzymes shape determines its function ,
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What happens if a proteins shape changes
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The active site may be distored or lost altogether, rendering the protein non-functioning.
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How does a protein function optimally?
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at a specific temperature, pH and salinity.
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A change in an enzymes environment can alter what rate
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The rate of product formation.
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If the amount of enzyme is not limiting, what will happen to the rate of substrate conversion into product?
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It should increase with increasing concentration of substrate.
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What happens if you keep adding substrate while keeping the enzyme concentration constant?
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The product will reach a maximum value, because all ezymes are occupied with substrate.
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What types of molecules compete with enzymes to occupy the active sites? and how can they do this
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competitive inhibitors, because they mimic the size, charge distribution and shape of the natural substrate
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What happens if there is the presence of a competitive inhibitor?
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There will be a reduction in the rate of product formation because the competitive inhibitor bound to the enzyme will not be converted into product.
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Which inhibitors do not bind to the active site, but alter its shape and hence the shape of its active site?
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non-competitive inbibitors.
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In the experiment, we are using which scale to measure temperature? What is RT
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Celcius, RT is room temperature.
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what is the substrate used in the Lab
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p-nitrophenyl phosphate
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In the control group, we use the deonized water in place of what?
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The enzyme solution.
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In the experiment, what is the inhibitor used?
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KH2PO4 (potassium dihydrogen phosphate)
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