Practical Title: The pros and cons of cell signalling experiments: risk assessments
Aims:
The aim of this experiment was to identify the risks associated with working in a laboratory by carrying out a risk assessment. The risk assessment instructed us how to safely work in a laboratory while being aware of these risks and what procedures to carry out if an accident happened in the laboratory.
Methodology:
Prior to the beginning of the risk assessment, laboratory coats, nitrile rubber gloves and labortartory goggles were worn to protect and shield from the dangerous chemicals that would be encountered during the assessment.
1. A preliminary laboratory talk was given to highlight precautionary measures that should be taken while examining the hazardous chemicals, for instance, not coming in contact with any chemical and informing the laboratory demonstrator if an occurrence like this happened.
2. A sheet was given out to the students giving a list of various aspects of each chemical that should be examined and noted on the page.
3. The chemicals placed around the laboratories were a mixture of hazardous and non-hazardous chemicals and students were instructed to examine each chemical. 4. The MSDS booklet beside each of the chemicals emphasised the dangers of working with each of the hazardous chemicals and the safety procedures that should be followed when dealing with each specific chemical. Emergency responses to accidents were also listed. Students were instructed to take particular note of these specifications. 5. After the chemicals were each examined, a risk assessment report was written up. C. Emergency Response Fire (consult relevant MSDS for further information) Extinguish fire with water spray, alcohol resistant foam, carbon dioxide or dry chemical extinguisher depending on type of fire. Wear a self-contained breathing apparatus Beware of the hazards of carbon oxides, hydrogen chloride gas and nitrogen oxides Contact the fire brigade First Aid Responses (consult relevant MSDS for further information) Contact a doctor Rinse eyes with water Breathe in fresh air Rinse mouth with water Wash with soap and water Take off contaminated clothes and shoes Do not induce vomiting Call a poison centre An MSDS must accompany all victims of exposure when seeking medical advice. Always consult an MSDS following an exposure to a hazardous agent. Spill Response (consult relevant MSDS for further information) Sweep up and shovel, ensure there is no dust Prevent further leakage Wear respiratory protection Do not allow chemicals to come in contact with the environment D. Further Risk Control Measures Required e.g. demarcation of working area; isolation of ignition sources; use of warning signage; the use of additional safety equipment; implementation of safe handling, transport and storage arrangements; availability of appropriate first aid equipment / antidotes; refer to UCD Chemical Agents Safety Manual for further guidance on risk control measures for handling chemical agents. Wear protective gear at all time in a laboratory, ie, lab coats, goggles and nitrile rubber gloves. Wear additional protective gear in special cases if working with particularly hazardous material, eg, face shield. Do not eat or drink in a laboratory as chemicals might be ingested. Do not place any objects from a laboratory in your mouth. Do not smoke in a laboratory as chemicals that are flammable might be ignited. Be cautious of the electrostatic charge that may be built up in certain circumstances. Do not use a phone or other personal items which may be …show more content…
Six hazardous chemicals were identified, those being hydrochloric acid, sodium hydroxide, sodium dodecyl sulfate, calcium chloride, choline chloride and ouabain octohydrate. The most common danger of working with these was accidental inhalation of the chemical, skin contact or ingestion. Had this occurred, medical help was to be sought, however, steps could be taken prior to medical help to alleviate the individual’s danger of being in harm. These steps include rinsing the eye or mouth with water if either has been affected, breathing in fresh air if inhalation of chemical occurred, removing contaminated clothes and shoes, washing contaminated body parts with soap and water, not inducing vomiting if ingestion has occurred, and if a large quantity was entered into the system a poison centre should be
Aims:
The aim of this experiment was to identify the risks associated with working in a laboratory by carrying out a risk assessment. The risk assessment instructed us how to safely work in a laboratory while being aware of these risks and what procedures to carry out if an accident happened in the laboratory.
Methodology:
Prior to the beginning of the risk assessment, laboratory coats, nitrile rubber gloves and labortartory goggles were worn to protect and shield from the dangerous chemicals that would be encountered during the assessment.
1. A preliminary laboratory talk was given to highlight precautionary measures that should be taken while examining the hazardous chemicals, for instance, not coming in contact with any chemical and informing the laboratory demonstrator if an occurrence like this happened.
2. A sheet was given out to the students giving a list of various aspects of each chemical that should be examined and noted on the page.
3. The chemicals placed around the laboratories were a mixture of hazardous and non-hazardous chemicals and students were instructed to examine each chemical. 4. The MSDS booklet beside each of the chemicals emphasised the dangers of working with each of the hazardous chemicals and the safety procedures that should be followed when dealing with each specific chemical. Emergency responses to accidents were also listed. Students were instructed to take particular note of these specifications. 5. After the chemicals were each examined, a risk assessment report was written up. C. Emergency Response Fire (consult relevant MSDS for further information) Extinguish fire with water spray, alcohol resistant foam, carbon dioxide or dry chemical extinguisher depending on type of fire. Wear a self-contained breathing apparatus Beware of the hazards of carbon oxides, hydrogen chloride gas and nitrogen oxides Contact the fire brigade First Aid Responses (consult relevant MSDS for further information) Contact a doctor Rinse eyes with water Breathe in fresh air Rinse mouth with water Wash with soap and water Take off contaminated clothes and shoes Do not induce vomiting Call a poison centre An MSDS must accompany all victims of exposure when seeking medical advice. Always consult an MSDS following an exposure to a hazardous agent. Spill Response (consult relevant MSDS for further information) Sweep up and shovel, ensure there is no dust Prevent further leakage Wear respiratory protection Do not allow chemicals to come in contact with the environment D. Further Risk Control Measures Required e.g. demarcation of working area; isolation of ignition sources; use of warning signage; the use of additional safety equipment; implementation of safe handling, transport and storage arrangements; availability of appropriate first aid equipment / antidotes; refer to UCD Chemical Agents Safety Manual for further guidance on risk control measures for handling chemical agents. Wear protective gear at all time in a laboratory, ie, lab coats, goggles and nitrile rubber gloves. Wear additional protective gear in special cases if working with particularly hazardous material, eg, face shield. Do not eat or drink in a laboratory as chemicals might be ingested. Do not place any objects from a laboratory in your mouth. Do not smoke in a laboratory as chemicals that are flammable might be ignited. Be cautious of the electrostatic charge that may be built up in certain circumstances. Do not use a phone or other personal items which may be …show more content…
Six hazardous chemicals were identified, those being hydrochloric acid, sodium hydroxide, sodium dodecyl sulfate, calcium chloride, choline chloride and ouabain octohydrate. The most common danger of working with these was accidental inhalation of the chemical, skin contact or ingestion. Had this occurred, medical help was to be sought, however, steps could be taken prior to medical help to alleviate the individual’s danger of being in harm. These steps include rinsing the eye or mouth with water if either has been affected, breathing in fresh air if inhalation of chemical occurred, removing contaminated clothes and shoes, washing contaminated body parts with soap and water, not inducing vomiting if ingestion has occurred, and if a large quantity was entered into the system a poison centre should be