In 1859, Charles Darwin documented that evolution of life on Earth had taken place and proposed the idea that natural selection was to blame in his book, The Origin of Species (Leicht and McAllister, 2016). Darwin’s findings arose from the different heritable traits, which lead to differences among alleles in a population. Genetic variation is the differences in genetic frequencies in a population and is the basis of evolution. The Hardy Weinberg Principle was developed to test for genetic variation in a given population. The Hardy Weinberg Principle states that if no evolutionary change is acting on a population, than the allele and genotype frequencies will remain the same. In order for the model to be accurately applied, a population
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In order to do this, we were provided a DNA extraction kit. The kit contained a collection swab which each member placed in their mouth to gather the cheek cells and then proceeded to transfer the swab to a test tube where the swab was rotated around the tube 10 times. The test tube contained the DNA extraction solution. The test tube was then vortexed for 10 seconds, incubated for 1 minute at 65℃, vortexed for 15 seconds, incubated for 2 minutes in a heat block at 98℃, and vortexed for another 15 seconds before placing the test tube on ice (Leicht and McAllister, …show more content…
The gels are made of agarose. To begin, we used .48 grams of agarose to prepare 30µl of a 1.6% agarose solution. We then transferred the weighted agarose to a 125-ml Erlenmeyer flask and added 30 ml of 1X TBE buffer. The agarose was then heated in a microwave for 30 seconds, before adding ethidium bromide which will stain the DNA fragments as they move through the gel. After mixing the agarose and bromide, we constructed the chamber and proceeded to carefully pour the agarose into the chamber. After pouring the agarose, we proceeded to add 1X TBE buffer to the chamber. To load and run Gel 1, we added 6µl of 10X Blue Juice to our PV92 tube. We then obtained a tube of DNA size markers which contained known DNA fragments of 766, 500, 300, 150, and 50 base pairs which were added to Well 2 and measured at 10µl (Leicht and McAllister, 2016) . We then proceeded to fill wells 3-5 with 20µl of our PV92 samples, each teammate getting their own well. We then ran the gel for 60 minutes at high voltage, when completed the gel was photographed. To load and run Gel 2, much of the same process was performed in which we added 3µl of 10X Blue Juice to our TAS2R38 tube. We then loaded well 1 of the gel with the DNA size marker at 10µl. Wells 2 and 6 were loaded with undigested samples while wells 3-5 were loaded with 10µl of each member’s sample. The gel was then ran on the high
In order to do this, we were provided a DNA extraction kit. The kit contained a collection swab which each member placed in their mouth to gather the cheek cells and then proceeded to transfer the swab to a test tube where the swab was rotated around the tube 10 times. The test tube contained the DNA extraction solution. The test tube was then vortexed for 10 seconds, incubated for 1 minute at 65℃, vortexed for 15 seconds, incubated for 2 minutes in a heat block at 98℃, and vortexed for another 15 seconds before placing the test tube on ice (Leicht and McAllister, …show more content…
The gels are made of agarose. To begin, we used .48 grams of agarose to prepare 30µl of a 1.6% agarose solution. We then transferred the weighted agarose to a 125-ml Erlenmeyer flask and added 30 ml of 1X TBE buffer. The agarose was then heated in a microwave for 30 seconds, before adding ethidium bromide which will stain the DNA fragments as they move through the gel. After mixing the agarose and bromide, we constructed the chamber and proceeded to carefully pour the agarose into the chamber. After pouring the agarose, we proceeded to add 1X TBE buffer to the chamber. To load and run Gel 1, we added 6µl of 10X Blue Juice to our PV92 tube. We then obtained a tube of DNA size markers which contained known DNA fragments of 766, 500, 300, 150, and 50 base pairs which were added to Well 2 and measured at 10µl (Leicht and McAllister, 2016) . We then proceeded to fill wells 3-5 with 20µl of our PV92 samples, each teammate getting their own well. We then ran the gel for 60 minutes at high voltage, when completed the gel was photographed. To load and run Gel 2, much of the same process was performed in which we added 3µl of 10X Blue Juice to our TAS2R38 tube. We then loaded well 1 of the gel with the DNA size marker at 10µl. Wells 2 and 6 were loaded with undigested samples while wells 3-5 were loaded with 10µl of each member’s sample. The gel was then ran on the high