Background
The American bullfrog, Lithobates catesbeianus, is an amphibian about the size of a teacup.(National Geographic, “American Bullfrog”). This dissection explored the anatomy of a bullfrog that had been pithed. Pithing is a technique used to prevent the frog from experiencing any cruel or unusual pain during the dissection. A pithed frog has had its nervous tissue in its brain severed thus allowing it to maintain normal bodily function during the dissection without having to endure the pain of the dissection.
Procedure
After obtaining a preserved frog, place the frog dorsal side up to examine its external anatomy. You should be able to locate the tympanum, eyes,, nares, and anus on the dorsal surface of the frog.