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53 Cards in this Set
- Front
- Back
Which tests use SIM's media?
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Indole test, H2S production, Motility test
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Describe how to perform an Indole test
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Inoculate sim's media, incubate, then add Kovac's reagent
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What is the distinctive media in an Indole test?
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trytophan
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What does a positive reaction of an Indole test look like?
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Red color at top
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What is the distinctive media in the H2S test?
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iron, sulfur
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What is a result for H2S test?
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Black precipate
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What does a positive result for a motility test look like?
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Growth away from stab, or turbidity
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How do you perform a nitrate test?
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Inoculate broth, intubate, add reactant
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What is the important parts of the media in a nitrate test?
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beef extract, peptone, potassium nitrate
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What is a positive results for a nitrate test?
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Red
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What is a result for H2S test?
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Black precipate
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What does a positive result for a motility test look like?
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Growth away from stab, or turbidity
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How do you perform a nitrate test?
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Inoculate broth, intubate, add reactant
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What is the important parts of the media in a nitrate test?
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beef extract, peptone, potassium nitrate
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What is a positive results for a nitrate test?
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Red
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What does the urea hydrolysis test for?
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The ability to hydrolyze urea to make ammonium
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What is the distinctive media for a urea hydrolysis test?
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Urea broth, phenol red, yeast extract
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What is a positive result for a urea hydrolysis test?
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Bright pink
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How do you perform an Oxidase test?
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Inoculate TSA w/organism, incubate and add oxidase reagent
OR add 1 drop tap water to oxi-strip and place 1 to colonies on applicator stick, read after 15 secs |
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What does does the Oxidase test?
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ability to use cytochrome oxidase
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What is a positive result for the Oxidase test?
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Colonies turn purple when exposed to oxidase reagent
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How do you perform a Catalase test?
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streak inoculate a TSA plate, intubate, add hydrogen peroxide
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What is a positive result of the Catalase test?
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If colonies bubble
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What do the Sugar Fermentations test?
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Ability to ferment sugar
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What is distinctive in the media for Sugar Fermentation test?
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Sugar, phenol red, durham tube
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What does a positive Sugar Fermentation test look like?
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Turned to yellow, and/or gas in durham tube
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How do you perform the Triple Sugar Iron Agar test?
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Stab slant and streak top
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What does the Triple Sugar Iron Agar test?
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Ability to ferment glucose and lactose/sucrose
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What are the distinctive media in Triple Sugar Iron Agar?
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phenol red, sugar, agar, ferrous sulfate
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What do the results look like for Triple Sugar Iron Agar?
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butt yellow/slant red glucose only KA
everything yellow, glucose + AA no change nothing K/NC black precipate sulfur H2S cracks produced CO2 gas |
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How do you perform the Methyl Red/Vogues Proskaver test?
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Inoculate, incubate and add reactant
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What does the MR/VP test?
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used to differentiate between species of enterobacteriacea. MR - acid from glucose VP - test for acetoin
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What does a positive MR and positive VP test look like?
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MR - result is red, pH is below 5
VP - result is red after addition of VP reagents |
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How do you perform a Citrate test?
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Inoculate green slant and ferment
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What does Citrate test?
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if bacteria uses citrate as sole carbon source and nitrogen as sole source
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What are the distinctive media in a Citrate test?
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simmon's citrate agar, pH indicator
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What does a positive Citrate test look like?
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Media turns blue
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How do you perform a Starch Hydrolysis test?
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Inoculate plate, incubate, flood plate with iodine
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What does the Starch Hydrolysis test?
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Ability of bacteria to hydrolyze starch with amylase
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What does a positive Starch Hydrolysis test look like?
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Clear ring around colonies
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What is in distinctive media in the Litmus Milk test?
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skim milk, pH indicator
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What are the 4 things the Litmus Milk tests?
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Lactose fermentation, reduction of litmus, curd formation and peptonization
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What does a pink test mean? LM
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lactose fermenation
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What does a white test mean? LM
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reduced litmus
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What does a purple test mean? LM
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oxidized litmus
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What does a brownish test mean? LM
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peptonization
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What does a blue test mean? LM
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digestion of casein
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What does a purple or blue ring of color mean? LM
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alkaline reaction
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What does clumping mean? LM
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accumulation of acid and to create casein clump
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What does a brownish test mean? LM
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peptonization
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What does a blue test mean? LM
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digestion of casein
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What does a purple or blue ring of color mean? LM
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alkaline reaction
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What does clumping mean? LM
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accumulation of acid and to create casein clump
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