Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
31 Cards in this Set
- Front
- Back
Catalase
|
breaks down peroxide into H2O and O2
|
|
Catalase reaction with Staph.
|
positive
|
|
Catalase reaction with Strep.
|
negative
|
|
What type of plate is manitol salt agar
|
selective (contains
NaCl) and differential (contains mannitol) |
|
What bacteria shows positive reaction to MSA?
|
S. aureus ferments
mannitol-yellow halo |
|
What staph infection is coagulase positive?
|
staph aureus
|
|
What staph infection in coagulase negative?
|
staph epi.
|
|
blood agar
|
– enriched media
– fastidious organisms |
|
Beta hemolysis seen on blood agar
|
– blood cells are
completely destroyed – S. pyogenes |
|
Alpha hemolysis seen on blood agar
|
– red blood cells are
partially destroyed – Medium turns olive green to brown – S. pneumonia |
|
Gama hemolysis seen on blood agar
|
no hemolysis visible
|
|
What is a mitus-salivarius medium?
|
medium used to grow oral cavity strep.
|
|
What does mitus salivarius medium contain?
|
tellurite
|
|
What color will the colonies be on a mitus salivarius medium
|
blue
mitis will produce pinpoint colonies salivarius will produce gumdrop colonies |
|
What is chocolate agar?
|
– cooked blood agar
– enriched media (not selective or differential) |
|
what bacteria is a chocolate agar used on?
|
neisseria
|
|
What are the major families of enteric bacteria?
|
– Salmonella
• typhoid fever – Shigella • shigellosis – Escherichia • traveler’s diarrhea |
|
Is neisseria oxidase positive or negative?
|
positive
|
|
What is MacConkeys agar used for ?
|
enteric bacteria
|
|
What are the characteristics of MacConkeys agar?
|
Selective
– inhibits growth of Gram (+) • Bile salts • Crystal violet • Differential – lactose • Lactose Fermenters – pink to brick red colonies |
|
What are the characteristics of Eosin-methylene Blue agar?
|
• Selective
– dyes inhibit Gram (+) • Differential – lactose • Lactose Fermenters – pigmented colonies • Non-Fermenters – colorless colonies |
|
What does EMB agar do to E. coli?
|
Black colonies eith a green metalic sheen
|
|
What is the effect of Ultra-Violet Light to bacteria?
|
• causes production of thymine dimers
– DNA incapable of base pairing – prevents transcription of DNA into RNA • Protein synthesis interrupted = death • Lethal and non-lethal mutations |
|
What is the wavelength of uv light used to mutate bacteria?
|
• Wavelength of 265 nanomenters
|
|
What type of agar do fungi prefer? And Name
|
• low pH & high sugar concentration
- Sabouraud’s Dextrose Agar |
|
Deuteromycota
|
• Penicillum
• Asexual Reproduction – conidiospores |
|
Deuteromycota
|
Aspergillus
– Asexual reproduction • conidiospores |
|
Basidiomycota (Club Fungi)
|
• Mushrooms
• Asexual spores – Conidiospores • Coprinus • Sexual spores – Basidiospores |
|
Zygomycota (bread mold)
|
• FamilyRhizopus
– conjugation fungi • Asexual reproduction – sporangiosporess |
|
Rhizopus
|
• Asexual reproduction
– sporangiosphores |
|
Ascomycota (sac fungi)
|
• Ascospores
– 8 in ascus |