Micro Lab Midterm

Front 1

Identify microbe.

Yeast or mold?

Disease or use?

Back 1

Candida Albicans

Yeast

Yeast, thrush, UTIs

Front 2

Identify microbe.

Yeast or mold?

Disease or use?

Back 2

Aspergillus spp.

Mold

Skin infections

Front 3

Identify microbe.

Yeast or mold?

Disease or use?

Back 3

Penicillium

Mold

Antibiotic penicillum

Front 4

Name fungus and reproduction

Yeast or mold?

Disease or use?

Back 4

Rhizopus - asexual

Mold

Skin infections

Front 5

Name fungus and reproduction

Yeast or mold?

Disease or use?

Back 5

Rhizopus - sexual

Mold

Skin infections

Front 6

Identify fungus

Yeast or mold?

Disease or use?

Back 6

Saccharomyces cerevisiae

Yeast

Bread yeast

Front 7

What type of stain is an Acid-Fast Stain?

Back 7

Differential

Front 8

What type of stain is Capsule Stain?

Back 8

Structural

Front 9

In a capsule stain, what stains pink and what stains white?

Back 9

Background and cell is pink

Capsule is white

Front 10

Name two types of mycobacterium

Back 10

Tuberculosis and leperosy

Front 11

What are the benefits of having a capsule?

Back 11

Offer extra protection

Help form biofilms

Retain water

Front 12

Acid-Fast cells have waxy component to their cell walls called

Back 12

Mycolic Acid

Front 13

The mycolic acid in the outer cell wall of an acid-fast cell are beneficial to the cells how?

Back 13

Retain water

Resistant to things they are exposed to

Front 14

What color does the Acid-Fast cell stain?

If they are not Acid Fast, what color will they be?

Back 14

Red (gram will be pinker)

Blue (gram will be dark purple color)

Front 15

What type of stain is an endospore stain?

Back 15

Differential stain

Front 16

Endospores contain ____________ & ______________

Back 16

DNA and ribosomes

Front 17

In an endospore stain, what color does the endospore and cell stain?

Back 17

Endospore stains light blue

Cell stains pink

Front 18

What 2 genera of microbes make endospores

Back 18

Bacillus

Clostrididium

Front 19

What type of stain is a flagella stain?

Back 19

Structural

Front 20

What type of flagella arrangement is this?

Back 20

Lophotrichous

Front 21

What type of flagella arrangement is this?

Back 21

Amphitrichous

Front 22

What type of flagella arrangement is this?

Back 22

Monotrichous

Front 23

What type of flagella arrangement is this?

Back 23

Peritrichous

Front 24

What type of stain is this?

Pink?

Purple?

Back 24

Gram

Pink: Gram - Cell membrane, thin cell wall, outer membrane

Purple: Gram + cell membrane, thick cell wall

Front 25

What cell arrangement is this?

Back 25

Streptococcus

Front 26

What type of cell arrangement is this?

Back 26

Palisade

Front 27

Basic stains are applied to bacterial smears that have been ___________

Back 27

Heat-fixed

Front 28

cell morphology, size, and arrangement can be determined using this type of stain

Back 28

Simple

Front 29

The most commonly used differential stain

Back 29

Gram

Front 30

What type of stain is this?

Back 30

Gram

Front 31

leaving the decolorizer on too long and get reddish Gram-positive cells is called

Back 31

Over-decolorize

Front 32

When you produce purple Gram-negative cells the mistake you made was...

Back 32

Under-decolorized

Front 33

What is the time limit for doing a gram stain on organisms

Back 33

Under 24 hrs

Front 34

Gram positive cells will appear what color?

Back 34

Purple

Front 35

Gram negative cells will appear what color?

Back 35

Pink

Front 36

What type of stain is this?

Back 36

Acid-Fast

Front 37

What type of stain is this?

Back 37

Acid-Fast

Front 38

What type of stain is this?

Back 38

Capsule

Front 39

composed of mucoid polysaccharides or polypeptides that repel most stains because of their neutral charge

Back 39

Capsules

Front 40

What type of stain is this?

Back 40

Capsule

Front 41

The total absence of ________ within endospores is an indication of how dormant they are

Back 41

ATP

Front 42

What type of stain is this?

Back 42

Endospore

Front 43

What type of stain is this?

Back 43

Endospore

Front 44

What type of stain is this?

Back 44

Endospore

Front 45

What three places can the endospore be located within the cell?

Back 45

Terminal

Middle

Subterminal

Front 46

What type of stain is this?

Back 46

Endospore

Front 47

What type of stain is this?

Back 47

Flagella

Front 48

Cannot make their own food

Back 48

Heterotrophs

Front 49

Can have two forms

Back 49

Dimorphic

Front 50

branch like structures on fungus

Back 50

Hyphae

Front 51

Mold reproduces by ____________ either asexually or sexually

Back 51

spores

Front 52

Where are spores located?

Back 52

End of hyphae

Front 53

Why are yeast cells much larger than bacteria

Back 53

More organelles and they sprout

Front 54

What yeast is this? And what is the arrow pointing to?

Back 54

Saccharomyces cerevisiae

Budding cell

Front 55

What type of yeast is this?

Back 55

Candida albicans

Front 56

A single fungal filament is called a

Back 56

hyphae

Front 57

What are the black lines pointing to?

Back 57

Top Line: Hyphae

Bottom Line: Spores

Front 58

Name protozoan and form it is in

Back 58

Entamoeba Histolytica

Cyst

Front 59

Name protozoan and form

Back 59

Toxoplasma Gondii

Front 60

Name protozoan and form

Back 60

Giardia lamblia

Cyst

Front 61

Name protozoan and form

Back 61

Giardia lamblia

Trophozoite

Front 62

Name protozoan

Back 62

Trichomonas vaginalis

Front 63

Name protozoan

Back 63

Trypanosoma gambiense or brucei

Front 64

Name protozoan

Back 64

Balantidium coli

Front 65

single celled animal like protists

Back 65

Protozoans

Front 66

How do you contract Giardia lamblia?

Back 66

Drinking contaminated water

Front 67

What does Giardia Lamblia cause and what type of smear do you find it in?

Back 67

Diarrhea

Fecal

Front 68

What does Toxoplasma gondii look like if located extracellularly?

Back 68

Crescent moon

Front 69

Contracted by cat feces or by eating undercooked red meat

Back 69

Toxoplasma gondii

Front 70

What symptoms does Toxoplasma gondii cause?

Back 70

Flu-like symptoms

Passes in utero to baby and it can go blind or develop mental disabilities

Front 71

How do you catch plasmodium vivax and what type of smear do you find it in?

Back 71

Mosquito

Blood smear

Front 72

What does plasmodium vivax cause?

Back 72

Malaria

Front 73

What is the other name for plasmodium vivax?

Back 73

Plasmodium falciparum

Front 74

Where do you find plasmodium vivax in the body?

Back 74

Inside red blood cells or the plasma

Front 75

What extracellular characteristics does giardia lamblia have?

Back 75

Flagella

Front 76

What extracellular characteristics does amoeba proteus have?

Back 76

Pseudopods

Front 77

Which of the protozoans that we studied is non-pathogenic

Back 77

Amoeba proteus

Front 78

Name the 3 pathogenic amoebas

Back 78

Naegleria fowleri

Acanthamoeba

Balamuthia mandrillaris

Front 79

Contracted by water rushing up the nose and the amoeba latches on to the olfactory nerves

Back 79

Naegleria fowleri

Front 80

What does Naegleria fowleri cause?

Back 80

Encephalitis

Front 81

How does naegleria fowleri move?

Back 81

Flagella and pseudopods

Front 82

Acanthamoeba usually exists in _______ form but when it comes in contact with the ________ it turns into a ___________ which can lead to __________

Back 82

cyst

eye

trophozoite

blindness

Front 83

This amoeba can be inhaled from dust/dirt and make its way to the brain from the lungs

Back 83

Balamuthia mandrillaris

Front 84

What disease does balamuthia mandrillaris cause?

Back 84

Encephalitis

Front 85

How do you contract Entamoeba histolytica and in what kind of smear do you find it in?

Back 85

Drinking contaminated water

Fecal smear

Front 86

How does Trichomonas vaginalis move?

Back 86

Flagella

Front 87

What does Trichomonas vaginalis cause and in what type of smear do you find it in?

Back 87

Trichomoniasis

But usually asymptomatic

Vaginal

Front 88

How does Balantidium coli move?

Back 88

Cilia

Front 89

What disease does Balantidium coli cause and what kind of smear do you find it in?

Back 89

Diarrhea

Fecal

Front 90

How do you contract Balantidium coli?

Back 90

Drinking contaminated water

Front 91

How do you contract Trypanosoma gambiense (brucei) and what smear do you find it in?

Back 91

TseTse fly or Kissing Bug (know both)

Blood

Front 92

How does Trypanosoma gambiense (brucei) move and what diseases does it cause?

Back 92

Flagella

African Sleeping Sickness and Chaga's Disease (know both)

Front 93

Name forms the following protozoans exist in:

Giardia lamblia

Toxoplasma gondii

Plasmodium vivax

Naegleria fowleri

Acanthamoeba

Balamuthia mandrillaris

Entamoeba histolytica

Trichomonas vaginalis

Balantidium coli

Trypanosoma brucei

Back 93

All exist in both forms only Trichomonas exists in

Trophozoite Form

Front 94

Name protozoan

Back 94

Toxoplasma gondii

Front 95

Name protozoan and form

Back 95

Entamoeba histolytica

Cyst

Front 96

Name protozoan and form?

Back 96

Giardia lamblia

Cyst

Front 97

Name protozoan and form?

Back 97

Giardia lamblia

Trophozoite

Front 98

Name protozoan and form?

Back 98

Trichomonas vaginalis

Trophozoite

Front 99

Name protozoan and form?

Back 99

Balantidium coli

Front 100

Name protozoan and form?

What does it cause?

Back 100

Plasmodium falciparum

Trophozoite

Malaria

Front 101

Name protozoan and form?

Back 101

Amoeba proteus

Trophozoite