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6 Cards in this Set
- Front
- Back
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what is the correlation between corrected OD and cell number for your culture? |
gives a more accurate representation of cell number |
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When performing a standard plate count, why are the counts reported as colony forming units (CFUs) rather than cells? |
Each colony theoretically comes from one cell, but in reality each colony can form from a few cells in a cluster or chain. (Because of the uncertainty in how many actual cells form a colony.) |
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How would you inoculate a plate to get a 1:10 dilution? |
Mix 1 ml of a culture with 9 ml of water and plate 1 ml of the dilution Plate 0.1 ml of culture |
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How would you inoculate a plate to get a 1:100 dilution? |
Mix 1 ml of a culture with 9 ml of water and plate 0.1 ml of the dilution Mix 1 ml of a culture with 99 ml of water and plate 1 ml of the dilution. |
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Why is it necessary to perform a plate count in conjunction with the turbidimetry procedure? |
A plate count gives viable numbers while turbidimetry gives all particles, so living and dead cells. |
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For the following methods of bacterial enumeration, does the method determine total count or viable count? 1. MPN 2.Microscopic Count 3. Standard plate count 4. Turbidity |
1. viable count 2. total count 3. viable count 4. total count |
