Micro Lab 1

Front 1

Oculur lens

Back 1

Lens located in the eyepiece with 15x magnification

Front 2

Objectives

Back 2

Lens on revolving nosepiece 4x,10x,40x, and 100x magnification

Front 3

Mechanical stage

Back 3

Knobs that move the platform stage from side to side and forward and backward

Front 4

Condenser lens

Back 4

Lens system below stage that concentrate the light reaching the specimen.

Front 5

Coarse Adjustment knob

Back 5

Large knob on both sides towards base, used with 4x and 10x for initial focusing

Front 6

Fine adjustment knob

Back 6

Small inner knob on both sides next to coarse adjustment knob, used for fine focus with 40x and 100x objective

Front 7

Rheostat

Back 7

Knob that regulates the amount of light leaving the lamp

Front 8

Iris diaphragm

Back 8

Lever on condenser that regulates amount of light entering specimen

Front 9

15x __________ (4x) = 60x

Back 9

Scanner

Front 10

X_______(10x) = 150x

Back 10

Low power

Front 11

X______(40x) = 600x

Back 11

High and dry

Front 12

X ________ (100x) = 1500x

Back 12

Oil immersion

Front 13

Resolving power

Back 13

Minimum distance between 2 objects to distinguish them as separate & distinct. Maximum resolution of light is 0.2 micrometers

Front 14

Does resolving power affect our ability to differentiate two objects on a slide from each other?

Back 14

Yes

Front 15

Protoslo

Back 15

Slows down organisms on a microscope for observation

Front 16

disinfect the lab bench after a spill

Back 16

1. Inform instructor

2. Cover with paper towel

3. Spray with disinfectant

4. Let sit for 10 minutes

5. Re-clean with fresh paper towels

5. Trash in autoclave bin

Front 17

What BSL organisms used in class (levels only)

Back 17

1 & 2

Front 18

Proper lab attire

Back 18

Lab coat, goggles, gloves, hair pulled back, closed toe shoes

Front 19

Bio safety cabinet

Back 19

Use when working with BSL-2 liquid containers

Front 20

Person items

Back 20

Most be stored & locked in a locker

Front 21

Media

Back 21

Provides space and nutrients for microbes to grow

Front 22

General purpose media

Back 22

Anything can grow on it as long as it doesn’t need anything special

-TSA/TSB

-Brain heart infusion (BHI), nutrient broth

Front 23

Enrichment

Back 23

Needs growth hormones/factors or special nutrients

-Blood agar (blood cells broken down and nutrients utilized

Front 24

Selective

Back 24

Inhibits he growth of one or more organisms in order to promote the growth of others

-SDA, MCA, HEA,

Front 25

Enrichment

Back 25

Needs growth hormones/factors or special nutrients

-Blood agar (blood cells broken down and nutrients utilized

Front 26

Selective

Back 26

Inhibits the growth of one or more organisms in order to promote the growth of others

-SDA, MCA, HEA,

Front 27

Differential

Back 27

Helps distinguish groups of organisms from each other based on biochemical characteristics

-Blood agar, EMB,MCA, HEA etc

Front 28

Commensal organisms

Back 28

Microbes that inhibit our bodies in ways that may benefit both them and us. We have symbiotic relationship

Front 29

Fomite

Back 29

Person or object that mechanically carries microbes from one person/ place to another

Front 30

Hand washing

Back 30

Wash hands before and after lab

Wet hands

Apply soap to cover hands

Wash for 15 seconds

Rub palms together and interlace fingers and thumbs

Rub top and bottom of hands and fingernails

Than rinse hands

Dry hands

Use towel to turn off water