Medical Laboratory Technology Flash Card

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Catalase positive ; Coagulase negative causes UTI in young sexually active females

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Staphylococcus saprophyticus

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Catalase positive ; Coagulase positive

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Staphylococcus aureus

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Gram positive cocci in clusters

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Staphylococcus

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Gram positive cocci in pairs or chains
Catalase negative

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Streptococcus

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Non-pathogenic organism that is catalase positive ; Coagulase negative Bacitracin sensitive

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Micrococcus

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Beta hemolytic creamy white colonies that ferments mannitol

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Staphylococcus auerus

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Catalase positive
White non-hemolytic colonies
Coagulase negative

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Staphylococcus

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Group A beta-hemolytic wide zone
Bacitracin sensitive
causes strept throat rheumatic fever and glomerulonephritis

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Streptoccus pyogenes

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Group B Beta-Hemolytic narrow zone Resistant to Bacitracin
normal flora of female genital tract

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Streptococcus agalactiae

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Diplococci lancet shaped
Alpha hemolytic
cause pneumonia and meningitis

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Streptococcus pneumoniae

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Group A Beta hemolytic streptococci infection occurring in children who have throat infections or skin infections can cause

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acute glomerulonephritis

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Haemophilius can become immune to ampicillin because of

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beta lactamase

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Thioglycollate broth is stored at room temperature and in the dark to

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decrease absorption of O2 by the medium

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Salmonella vs. Shigella

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Salmonella has motility while shigella is nomotile

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Liquid fecal specimen from infant should be tested for

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Salmonella-
Shigella
Campylobacter jejuni

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enterohemorrhagic Escherichia Coli

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causes hemolytic uremic syndrome

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Enteropathogenic E.Coli

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causes infantile (infancy) diarrhea

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Enterotoxigenis E. Coli

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traveler's diarrhea

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Enterinvasive E. Coli

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inflammation of the intestine or dystentery; bloody diarrhea

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Enteroaggregative E. Coli

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causes watery diarrhea ; community acquired

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Grows Hemophilius and Neisseria

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Chocolate Agar

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Fastidious bacteria

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Neisseria speices

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Grows Gram positive cocci

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PEA and Cloumbia Colistin Nalidixic Acid

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Selective for Gram negative bacteria differential for lactose fermenting rods

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MAC agar
Eosin-Methylene Blue

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Selective for N. gonorrheae and N. meningitidis

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Thayer-Martin Agar

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Selective for N. gonorrheae

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Jembec

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Selective for c. diphtheriae

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Cystine -Tellurite Blood and Tinsdale Agar

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Enrichment- used to demorstate dimorphism of fungi

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Brain heart infusion agar with blood

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To back up anaerobes

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Thioglycolate broth

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Selective for legionella species

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Buffered Charcoal yeast Extract

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Selective for Vibrio

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Thiosulfate Citrate-Bile Salts

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Agar that causes C. diphtheriae enhanced grouping and metachromatic granules

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Loeffler's Meduim

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Differential for Candida species

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Cornmeal agar with Tween 80

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Isolation and differation of C. neofarmans

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Birdseed agar

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If there is a deterioration of methicillin discs

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it can cause an increased growth of any organism

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ability to test microorganism's ability to ferment sugars and to produce hydrogen sulfide

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TSI Slant

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hydrogen sulfide (H2S) reacts with ferrous sulfate in the medium to form what visible black precipitate

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ferrous sulfide

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Sulfide producing bacteria

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Edwardsiella
Salmonella
Proteus
Citrobacter

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In TSI, All lactose fermenters result in

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yellow slant/yellow butt (acid/acid reaction)

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In TSI non-lactose fermenters results in

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pink/yellow or yellow/yellow (if sucrose is fermented)

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blackening of the medium at the interface of butt and slant

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Salmonella species

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Failure to obtain anaerobiosis in anaerobe jar is due to

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the inactivation of the palladium- coated alumina catalyst pellets

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Which gram negative organism doesn't grow on MAC agar and is Oxidase postive, catalase postive, and non motile

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Pasteurella multicida

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Most frequently isolated non fermenting gram negative rod that is Beta-hemolytic oxidase postive catalase postive motile

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Pseudomonas aeruginosa

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Non-fermenting, Gram Negative Rods

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Acinetobacter
Pseudomonas aeruginosa
Burkholderia
Chryseobacterium
Stenotrophomonas

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Non fermenting, Gram Negative Rods that is Oxidase negative

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Acinetobacter
Stenotrophomonas

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Non fermenting Gram Negative Rods that doesn't cause nosocomal infections

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Chryseobacterium

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Gram-Positive Bacilli (rods)

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Bacillus Norcardia
Corynebacterium
Erysipelothrix
Lactobacillus
Listeria monocytogenes

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Gram-Positive Cocci (circular)

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Stapylococcus
Steptococcus
Enterococcus
Micrococcus
Leuconostoc

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Enterobacteriacease
Oxidase negative
H2S negative

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Morganella morganii

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types of Vibrio species

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Helicobacter pylori
Campylobacter jejuni
V. cholerae
V. vulnificus

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Gram-Negative Cocci

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Neisseria
Neisseria gonorrhea
Neisseria meningitis
Moraxella

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Gram-positive anaerobes

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Bifidobacterium
Actinomyces
Clostridium
Eubacterium
Peptostreptococcus
Propionibacterium

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Gram-Negative anaerobes

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Bacteroides
Fusobacterium
Porphyromonas
Prevotella
Veillonella

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Used to prepare a slide from a plate culture of a dermatophyte (Fungus) for microscopic observation

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Lactophenol cotton blue

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Used to stain fungus

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Lactophenol cotton blue

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Potassium hydroxide

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hastens the decomposition process of soft tissues (both animal and human), leaving behind only bones

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Gram-positive bacteria have a

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thick mesh-like cell wall made of peptidoglycan

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Stain blue when stained with gram stain

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Gram-positive

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Gram-negative bacteria also have an additional outer membrane which contains

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lipids

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The gram-positive cell remains purple and the gram-negative cell loses its purple color

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Alcohol used in gram stain

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Histoplasma capsulatum

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has tuberculate macroconidia 8-14 microns

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enzyme that results in resistance to the beta-lactam antibiotics

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Beta-lactamase

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Beta-lactam antibiotics are

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inhibtors of cell wall synthesis

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Penicillins

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Beta-lactams

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liquefy the mucus

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N-acetyl-Lcysteine i

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Ureaplasma urealyticum needs

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Sterols to grow

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Used as a source of iron

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ferric pyrophosphate

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bacterial infection that commonly is transmitted through sexual activity

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Ureaplasma urealyticum

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All specimens that have a cyst state recovered form fecal specimen for examination should be

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stored at refrigerator temperature

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For quality control testing for DNAse production

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E.coli and Serratia marcescens can be used

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Throat or nasopharyngeal swabs submitted of anaerobic culture

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should be rejected

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Penicillin is an antibiotic that

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inhibits cell wall synthesis

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Latex Agglutination

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helps distinguish Group B Strep form other beta hemolytic streptococci

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used to differentiate Group D enterococci

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6.5% NaCl

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Group D streptococcus is known as

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Enterococcus

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Growth on Bile esculin medium can help identify

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Streptococcus D

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Bacitracin test is useful for

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differentiating β-hemolytic Group A streptococci from β-hemolytic non-Group A streptococ

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Streptococcus B can be distinguished by

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latex agglutination

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All gram negative cocci are

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oxidase positive

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Gram negative cocci that grows on Chocolate agar that is both maltose positive and glucose positive

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Neiseria meningitides

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Chocolate agar base containing cancomycin, colistin, nystatin and trimethoprim is known as

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modified Thayer-Martin Agar

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KV-Laked Blood

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slective for Bacteroides species

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Sodium bicarbonate and sodium citrate are components of

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JEMBEC system

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an enriched medium for the selective isolation and cultivation of Neisseria species.

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Modified Thayer-Martin (MTM II) Agar

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Agar is an enriched selective medium used for the isolation and cultivation of pathogenics Neisseria species enriched with Lysed horse blood, horse serum, dextrose and yeast dialysate

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NYC medium

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Anti-microbial dilution tests

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can detect varying degrees of organism sensitivity and resistance

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clinical resistance to penicillin correlates with

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beta-lactamase production in Neisseria meningitidis

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Universally susceptible to penicillin

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Streptococcus pyogenes

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first generation cepalosporins can be represented by

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cephalothin

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Selective for Camplobacter

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Skirrow Agar

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CIN Agar

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selective for Yersinia

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Selective for isolation of Salmonella from stool

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Bismuth sulfite

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Campylobacter fetus

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must be incubated in a mircoaerophilic environment for optimal recovery

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used as treatment against the three main bacterial causes of meningitis: Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae

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chloramphenicol

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antibiotic, used to treat many types of bacterial infections, particularly those caused by Gram-negative organisms and not used for Neisseria gonorrhea

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gentamicin

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if the media contains Cycloheximide it will be comprised of which species of fungi

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cycloheximide

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Candida albicans vs. Crytococcus neoformans

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urease negative vs. pink urease positive

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Which fungi grows as brown colonies on niger seed agar?

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Crytococcus Neoformans

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Positive urease test that turns pink

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Cryptococcus species

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Beta lactamase testing

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Staph. Aureus as positve
H. Influenzae as negative

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A cell culture line commonly used for the recovery of Chalmydia trachomatis for specimens

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Mc Coy's

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disease of the adrenal glands most commonly caused by the bacterium Neisseria meningitidis

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Water-Friderichsen syndrome

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Bacteroides fragilis is the most common

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gram negative anaerobes

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Relapsing fever in humans is caused by

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borrelia recurrentis

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used to monitor bactericidal activity during antimicrobic therapy in cases of endocarditis

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serum bactericidal (combines the interaction of the pathogen, the antimicrobial agent, and the patient.)

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occurs when drugs can interact in ways that enhance or magnify one or more effects, or side effects, of those drugs

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Sherris synergism

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Taken to determine how quickly it is cleared from the blood

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tolerance

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immuno diffusion technique that is a virulence test performed upon Corynebacterium diphtheriae

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Elek test

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In a disk diffusion susceptibility test, zones of a larger diameter would result if

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the disk is placed on the inoculated media and left at room temperature for an hour before incubation

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Egg yolk agar with lecithinase

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Clostridium peringens