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15 Cards in this Set
- Front
- Back
What microscope allows light rays to pass through a slide/specimen and then through various lenses to the eye
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Brightfield microscope
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What does the microscope condenser do?
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collects and directs light from under the stage
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What is the diaphragm?
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part of the condenser that controls the amount of light coming through
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What is resolution?
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The ability to distinguish 2 adjacent points as separate objects based upon resolving power
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What 3 things determine resolution?
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numerical aperture, wavelength of light, and design of the condenser
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How do you increase resolution?
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decrease the wavelength, condenser at highest position so more light can enter, diaphragm should be opened a little for less contrast but higher numerical aperture, and immersion oil
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What is numerical aperture?
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The measure of a lens' ability to capture light coming from a specimen and use it to make the image; increases with the use of oil and the higher objectives
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What is the formula for the limit of resolution?
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D= wavelength/NA condenser+NA objective lens
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What is phase-contrast microscopy?
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allows one to view living transparent organisms using phase (wavelength shifts) principles of light to produce dark objects in a bright background
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What kingdom do protozoa belong to?
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Protista
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What eukaryotic microbes are unpigmented and motile?
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protozoa
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What eukaryotic microbes are greenish or golden brown?
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algae
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What kingdom do algae belong to?
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Protista
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What organisms are blue-green?
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Cyanobacteria
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What kingdom to cyanobacteria belong to?
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Monera
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