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30 Cards in this Set
- Front
- Back
Myeloperoxidase stain (MPO)
|
Mature Neutrophils and Eos pos
Monos slightly pos Lymphs neg |
|
Cyanide-resistant peroxidase
|
Eos positive
Others neg |
|
Acid Phosphatase
|
T-cells pos
B-cells neg |
|
Tartrate-resistant Acid Phosphatase (TRAP)
|
Hairy-cells positive
|
|
Leukocyte Alkaline Phosphatase (LAP)
|
Only neutrophils pos
Score indicates maturity |
|
Periodic-Acid-Schiff
|
Neutrophils strong/Monos weak pos
Lymphs/erythroblasts neg Stains glycogen pink |
|
Sudan Black B
|
Granulocytes pos
Lymphs Neg Stains phospholipids/lipoproteins blue |
|
Non-specific Esterase (NSE)
|
Monos pos
(alpha-napthyl esterase) stains black |
|
Naphthol AS-D chloroacetate esterase
|
Neutrophils pos
Basos weak pos/neg Lymphs/Monos/Eos neg |
|
Prussian Blue
+siderocyte formula |
Precipitates free iron into granules
%siderocytes=#RBCs pos/100 RBCs Reference: 0-1% |
|
Chediak Hegashi Syndrome
|
Betaglucocerebrosidase deficiency
large lysosome granules/dohle bodies/partial albinism |
|
Gaucher's Disease
|
lipid storage disease
(large monocytes) |
|
Tay-Sachs Disease
|
hexosaminidase A def.
(monos) |
|
Niemann-Pick
|
sphingomyelinase def.
(monos) |
|
CD4
|
T-Helper
|
|
CD8
|
T-suppressor
|
|
ALL - FAB L1
|
pre-B cells
|
|
ALL - FAB L2
|
Adult T-cell
|
|
ALL - L3
|
Burkitt's lymphoma - leukemia phase (B-cells)
|
|
CLL
|
B-cells
|
|
Hairy-cell Leukemia
|
B-cells
TRAP Positive |
|
Waldenstrom's Macroglobulinemia
|
Plasma (B) Cell malignancy
M-protein |
|
Hodgkin's Lymphoma
|
Reid-Sternberg cells
|
|
AML - M1
|
90% Myeloblasts
|
|
AML - M2
|
<90% myeloblasts w/Auer Rods
|
|
AML - M3
|
Promyelocytic
Auer Rods |
|
AML - M4
|
AMML 30 myeloblasts in marrow
M4e - w/eosinophilia |
|
AMoL - M5
|
Monocytic
DIC and renal insuff. |
|
CMML
|
Myelodysplastic
<30% Blasts in periph. Alpha-napthyl esterase pos |
|
AEL - M6
|
Di Guglielmo's disease
PAS Positive |