Hema Secondary Hemostasis Ajfb

Front 1

Also known as enzyme precursors or zymogens

Found in Plasma along w/ nonenzymatic cofactors and calcium

Back 1

Coagulation Factors

Front 2

Substrates having no Biologic Activity

Back 2

Zymogens

Hint 2

II, VII, IX, X, XI, XII, prekallikrein

(2,7,9,10,11,12)

Front 3

Zymogens converted by enzymes to active forms

Back 3

Serine Protease

Hint 3

IIa, VIIa, IXa, Xa, XIa, XIIa & Kallikrein

Front 4

Cofactors that assist in the activation of zymogens

Back 4

1. V

2. VIII

3. Tissue Factor

4. HMWK

Hint 4

5, 8, 3

Front 5

Factor XIII in its active form is a

Back 5

Transglutaminase

Front 6

Only substrate in the cascade that does not become an activated enzyme

Back 6

Fibrinogen or

Factor 1

Front 7

Blood factors are produced mostly in the

Back 7

Liver

Front 8

Factor 1

Back 8

Fibrinogen

Hint 8

Most Concentrated

<10 mg/dL

Front 9

Factor II

Back 9

Prothrombin

Hint 9

Prethrombin

*80% Consumed during coagulation

20% Residual Prothrombin

Front 10

Factor III

Back 10

Tissue Factor

Hint 10

Tissue Thromboplastin

Front 11

Factor IV

Back 11

CALCIUM

Front 12

Factor V

Back 12

Proaccelerin

Hint 12

Labile Factor

Accelerator Globulin (Acg)

*80% in Plasma

Parahemophilia

Front 13

Factor VII

Back 13

Proconvertin

Hint 13

Stable Factor

Serum Prothrombin Conversion Accelerator (SPCA)

Front 14

Factor VIII:C

Back 14

Antihemophilic Factor

(AHF)

Hint 14

Antihemophilic Factor Globulin (AHG)

Antihemophlic Factor A

Platelet Cofactor 1

* Bethesda Assay

- Factor 8 Inhibitor

* Classic Hemophilia/Royal Disease

Front 15

Factor IX

Back 15

Plasma Thromboplastin Component (PTC)

Hint 15

Christmas Factor

Antihemophilic Factor B

Platelet Cofactor 2

Front 16

Factor X

Back 16

Stuart-Prower Factor

Hint 16

Stuart Factor

Prower Factor

Autoprothrombin III

Front 17

Factor XI

Back 17

Plasma Thromboplastin Antecedent (PTA)

Hint 17

Anti-Hemophilic Factor C

*Rosenthal Syndrome

Front 18

Factor XII

Back 18

Hageman Factor

Hint 18

1. Glass Factor

2. Contact Factor

Front 19

Factor XIII

Back 19

Fibrin Stabilizing Factor

Hint 19

1. Laki-lorand Factor

2. Fibrinase

3. Plasma Transglutaminase

4. Fibrinoligase

Front 20

Prekallikrein

Back 20

Fletcher Factor

Front 21

High Molecular Weight Kininogen (HMWK)

Back 21

1. Fitzgerald Factor

2. Contact Activation Cofactor

3. William's Factor

4. Flaujeac Factor

Front 22

Common Pathway Factors

Back 22

I, II, V, X

(1, 2, 5, 10)

Front 23

Intrinsic Pathway Factors

Back 23

VIII, IX, XI, XII

(8, 9, 11, 12)

Front 24

Extrinsic Pathway Factors

Back 24

VII (7)

Front 25

Fibrinogen Group

Back 25

I, V, VIII, XIII

(1, 5, 8, 13)

Front 26

Prothrombin Group

Back 26

II, VII, IX, X

(2, 7, 9, 10)

Front 27

Contact Group

Back 27

XII, XI, Prekallikrein, HMWK

Front 28

Fibrinogen Group Characteristics:

Back 28

1. Not Vit K dependent

2. Calcium Dependent

3. Present Plasma

Absent Serum

4. Not Adsorbed by BaSO4

Front 29

Prothrombin Group Characteristics:

Back 29

1. Vitamin K Dependent

2. Calcium Dependent

3. Present in Plasma & Serum (exc: II)

4. Adsorbed by BaSO4

Hint 29

Also adsorbed by Aluminum Hydroxide

Front 30

Contact Group Characteristics:

Back 30

1. Not Vit K dependent

2. Not Calcium Dependent

3. Present in Serum & Plasma

4. Not Adsorbed by BaSO4

Front 31

Degrades factor

Va & VIIIa

Back 31

Protein

C & S

Front 32

Major inhibitor of Thrombin

Also inhibits Factors 9a, 10a, 11a, 12a, kallikrein and Plasmin

Back 32

Antithrombin III

Hint 32

Also Inhibits Thrombin:

1. Heparin Cofactor II

2. A2 Macroglobulin

3. A1 Antitrypsin

Front 33

Forms a complex w/ thrombin, kallikrein, & plasmin, thus inhibiting their activities

Back 33

A2 Macroglobulin

Front 34

Inhibits 7a-Tissue Factor Complex

Back 34

Extrinsic Pathway Inhibitor (EPI)

Lipoprotein Associated Coagulation Inhibitor (LACI)

Front 35

Inactivator of

Factor 12, & Kallikrein,

also inhibits

Factor 11a & Plasmin

Back 35

C1 Inhibitor

Front 36

Inhibitor of Thrombin, 10a, 11a

Back 36

A1 Antytrypsin

Front 37

Most Common Acquired Coagulopathy

Back 37

Acute Coagulopathy of Trauma Shock (ACOTS)

Hint 37

Accounts for most fatal hemorrhage

Front 38

Acquired Coagulopathies:

Back 38

1. Liver Dse

2. Vit K Def

3. Renal Failure

Front 39

Most Common

Congenital Deficiencies:

Back 39

1. vWD

2. Factor 8 & 9 Def

3. Plt Function Disorder

Front 40

Most common congenital bleeding disorder

Most Frequently inherited Coag Disorder

Back 40

vWD

Front 41

Measures period required for free formation of blood clot after it has been removed from the body

Back 41

Coagulation/Clotting Time

Front 42

Coag specimen should be centrifuged w/in

Back 42

1 hr after obtaining the sample

Centri for 15 mins @2500g (Rodak: 1500g)

Hint 42

Processing:

1. W/in 4 Hrs for APPT

2. W/in 24 Hrs for PT

Front 43

Factors that will breakdown at temperatures above 37C

Back 43

5 & 8

Hint 43

LABILE FACTORS

Front 44

Factors that will be activated at Cold Temperatures

Back 44

7 & 11

Front 45

Useful screening procedure for the extrinsic coag mechanism and the common pathway

Back 45

Prothrombin Time (PT)

Hint 45

Will be prolonged if fibrinogen level is 100 mg/dL or Less (Rodak)

<80 mg/dL (Brown)

Front 46

PT Reagent

Back 46

Thromboplastin-

Calcium Chloride

Hint 46

Added to PPP obtained from Citrated Blood

Front 47

Screening for factors involved in Intrinsic & Common Pathway

Monitors Unfractioned Heparin Therapy

Back 47

Activated Partial Thromboplastin Time (APTT)

Hint 47

Activators:

1. Kaolin

2. Celite

3. Silica

4. Ellagic Acid

Front 48

Used to detect deficiencies in the Common Pathway Factors

Back 48

Stypven Time/Russel Viper Venom Time

Hint 48

East Indian Viper Venom/

Vipera Russelli

- A thromboplastin-like substance that activates factor X

Front 49

Stypven Reagent

Back 49

Platelin-

Calcium Chloride

Hint 49

Added to PPP obtained from Citrated Blood

Front 50

Detects availability of Functional Fibrinogen

Affected by Heparin

Back 50

Thrombin Time

Front 51

Reagent for

Thrombin Time

Back 51

Thrombin-

Calcium Chloride Reagent

Front 52

Unaffected by Heparin

Helpful in testing functional Fibrinogen when TT is prolonged due to Heparin

Back 52

Reptilase Time

Front 53

Reagent for

Reptilase Time

Back 53

Atroxin

Hint 53

From Bothrops atrox snake

Front 54

Screening for

Factor XIII Deficiency

Back 54

Duckert's Test/

5 M Urea Solubility Test

Front 55

Reagents used in

Duckert's Test

Back 55

1. 5 M Urea

2. 2% Acetic Acid

3. 1% Monochloroacetic Acid

Hint 55

If Factor XIII is absent, the clot is dissolved in less than 24 Hrs by urea

Front 56

Prolonged PT & APTT not corrected

Inactivate an activated coagulation factor or block interaction b/w coag factors and platelets

Back 56

Circulating Anticoagulants