Dna Replication Lecture 4

Front 1

What are the 2 sugars involved in the Nucleotide?

Back 1

1) Ribose

2) Deoxyribose

Front 2

What are the bases involved in the Nucleotide?

Back 2

1) Purine (AG)

2) Pyramidine (CUT)

Front 3

The base of the nucleotide is connected to _______ carbon via _______ bond.

Back 3

1) C1

2) Glycosidic

Front 4

What are the 3 phosphates involved in the Nucleotide?

Back 4

1) Alpha

2) Beta

3) Gamma

Front 5

What is an example of a cancer predisposition disorder?

Back 5

Bloom Syndrome

Front 6

What is an example a RecQ DNA Helicase Mutation?

Back 6

Bloom Syndrome

Front 7

The __________ is defective in Bloom's syndrome and is thought to be a part of the complex that unwinds DNA as two replication forks meet.

Back 7

DNA Helicase

Front 8

The DNA Polymerase EPSILON and DELTA have ________ activity.

Back 8

Exonuclease

Front 9

DNA polymerase can recognize the incorporation of __________ due to lack of _________.

Back 9

1) Wrong Bases

2) Watson Crick Base Pairing

Front 10

DNA Polymerase REMOVES _____________.

Back 10

Wrong Base

Front 11

Nucleosome = ________+________

Back 11

DNA + Protein

Front 12

Nucleosome has ______ Histone proteins (_____X each typical histone proteins).

Back 12

1) 8

2) 2

Front 13

_______bp DNA wrapped _________ around the 8 histone proteins.

Back 13

1) 150

2) Twice

Front 14

Nucleosomes are separated by _______bp known as ________.

Back 14

1) 50

2) Linker DNA

Front 15

What does NAP stand for?

Back 15

Nucleosome Assembly Protein

Front 16

The Nucleosome Assembly is broke down into 3 facts including:

1) Histones made by _________.

2) Histones are _________.

3) _______ facilitates nucleosome assembly.

Back 16

1) Ribosomes

2) Acetylated

3) NAP-1

Front 17

The acetylation of histones allows the histone to bind ________ to enter _______.

Back 17

1) Transport Protein

2) Nucleus

Front 18

The acetylation does NOT impact ______/______ with DNA.

Back 18

Gene Expression/Interaction

Front 19

Telomerase = ____________

Back 19

Ribonucleopeptide

Front 20

Ribonucleopeptide = ________ + ________

Back 20

Polypeptide + RNA template

Front 21

Telomerase recognizes _____' _____ REPEATS.

Back 21

3' Terminal

Front 22

Telomerase extends 3' end using _______.

Back 22

RNA template

Front 23

Telomerase adds _________.

Back 23

Deoxynucleotides

Front 24

What inhibits cyclin D1--CDK4 complex that leads to negative transcription?

Back 24

P16

Front 25

Which complex goes from:

Kinase --> phosphorylate Rb

No P16 --> active --> RB-Pi --> E2F

Active --> positive transcription

Back 25

Cyclin D1-CDK4 Complex

Front 26

During the Cyclin D1-CDK4 Complex,

Kinase --> ________

Back 26

Kinase --> Phosphorylate Rb

Front 27

During the Cyclin D1-CDK4 Complex,

No P16 --> ________ --> ________ --> _________

Back 27

No P16 --> active --> Rb-Pi --> E2F

Front 28

During the Cyclin D1-CDK4 Complex,

Active --> ________

Back 28

Active --> + Transcription

Front 29

Transactivator

+ transcription of proteins needed for S-phase

Back 29

E2F

Front 30

HYPO-phosphorylated --> inhibit E2F --> - transcription

HYPER-phosphorylated --> unbind E2F --> + transcription

Back 30

Rb

Front 31

HYPO-phosphorylated --> _______ --> _______.

HYPER-phosphorylated --> _______ --> _______.

Back 31

HYPO-phosphorylated --> inhibit E2F --> - transcription

HYPER-phosphorylated --> unbind E2F --> + transcription

Front 32

A CDK inhibitor.

Back 32

p16

Front 33

A G1-CDK

Back 33

Cyclin D1-CDK4 Complex

Front 34

An inhibitor of a gene regulatory protein.

Back 34

Rb

Front 35

A gene regulatory protein.

Back 35

E2F

Front 36

What are the steps of PCR?

Back 36

1) Isolate DNA

2) Heat

Denature (separate) dsDNA

3) Cool --> primers anneal

4) DNA Synthesis

5) Repeat