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69 Cards in this Set
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Nitric acid |
Most common and fastest |
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Nitric acid |
Recommended conc. of 5-10% |
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Nitric acid |
Inhibits nuclear stains and destroys tissues |
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Prevent damage by nitric acid |
Combine w/ formaldehyde or alcohol |
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Nitric acid |
Easily removed by 70% alcohol |
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Nitric acid |
Imparts yellow color |
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Formol-Nitric acid |
Less tissue destruction than 10% aqueous nitric acid |
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To prevent yellow color imparted by nitric acid |
Add 5% NaSO4 and wash with running tap water for at least 12 hrs |
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Make discoloration disappear |
Add 0.1% urea |
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Perenyi's fluid |
Decalcifying and tissue softener |
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Perenyi's fluid |
Maceration is avoided due to presence of chromic acid and alcohol |
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Perenyi's fluid |
Complete decalcification cant be determined by chem test |
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Prevent appearance of ppt in perenyi's fluid |
Add 0.5ml of saturated aqueous ammonium oxalate. Reappearance of ppt w/in 30 mins (decal is incomp) |
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Phloroglucin-Nitric acid |
The most rapid among decal acids |
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Phloroglucin-Nitric acid |
To remove acid, three changes of 70-90% ethanol since washing with water will make tissue swell and deteriorate |
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Phloroglucin-Nitric acid |
Slides are brought to water and placed in 1% lithium carbonate for 1 hr and washed for 15 mins them stained |
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HCl |
Also a softener. Produced good stain if 1% soltn with 70% alcohol for tissue blocks |
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Von Ebner's |
Good cytologic staining |
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Von Ebner's |
Dont require washing before dehy |
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Von Ebner's fluid |
Recommended for teeth and small pieces of bones |
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Von Ebner's fluid |
Decal cant be measured by chem test (same with perenyi's fluid) |
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Formic acid |
Decal of postmortem research tissues |
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Formic acid |
Addition of citrate with hasten decal process |
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Formic acid |
Only used when there is minimal decalcification is required |
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Formic acid |
Acts as fixative and decal |
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Formic acid |
Excellent nuclear and cytoplasmic staining |
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Formic acid |
Recommen for small pieces of bones and teeth. (Same with Von Ebner's fluid) |
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Formic acid |
Hasten if the vol is inc to 25ml. |
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Formic acid |
Req neutralization with 5%NaSO4 |
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Formic Acid-Sodium Citrate Soltn |
Recom for autopsy, BM, Cartilage, and tissue for research purp. |
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Trichloroacetic acid |
Dont req washing out. Excess acid removed by several changes of 90% alcohol, which improves tx dehy |
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Sulfuric acid |
Very weak decal only for minute pieces of bone |
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Chromic acid / Flemming's fluid w/ HAC |
Fix and decal agent (same with formic acid) |
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Chromic acid / Flemming's fluid w/ HAC |
Used for decal minute bone spicules |
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Chromic acid / Flemming's fluid w/ HAC |
Nclr stn w/ H&E is inhibited |
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Chromic acid / Flemming's fluid w/ HAC |
Tends to undergo reduction and forms ppt at bottom of container |
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Chromic acid / Flemming's fluid w/ HAC |
Insol pigments are formed |
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Chromic acid / Flemming's fluid w/ HAC |
Degree of decal cant be measured by routine chem test |
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Citric acid-citrate buffer soltn ph 4.5 |
Doesnt prod cell or tx distortion |
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Citric acid-citrate buffer soltn ph 4.5 |
Excellent nclr and cytopl stn |
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EDTA |
Small tx is placed in it for 1-3wk |
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EDTA |
Large tx is placed for 6-8wk |
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Calcium will not bind |
Below pH3 |
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Faster binding of Ca |
pH 7-7.4 |
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Optimal Ca binding |
pH 8 |
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EDTA |
Excellent decal agnt for immunohistochemical enzyme stng and for EM |
|
EDTA |
Inact ALP |
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To restore ALP act |
Add Magnesium Chloride |
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IER |
Hastens decal of formic acid containing decal agnt |
|
IER |
Nitric or HCl containing decal agnt is not recom |
|
IER |
1/2 is spread over the bot of container. 20-30x vol of tx |
|
IER |
Tx allowed to stay in soltn for 11-14 days |
|
IER |
Decal degree measured by phy or x-ray mtd |
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React Resin |
Immerse in N/10 HCl twice and wash w/ dist H20 3x |
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IER |
Washing of soltn is eliminated |
|
IER |
Minimal histological artifacts (same w/ EDTA) |
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Electrophoresis |
Satisfactory for small bone fragments |
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At 37°C |
Impaired staining of Van Gieson's stn for collagen fibers |
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At 55°C |
Undergo complete digestion w/in 24-48hrs |
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18-30°C |
Optimal temp for decal |
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24-48 hrs |
Ideal time for comp decal |
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Lithium carbonate |
Used in post decal |
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5-10% aqueous soltn. of Na bicarbonate |
Used in post decal(same w/ sat. lithium carbonate) |
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Post decal with H2O |
30 mins for small tx |
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Post decal with H2O |
1 to 4 hrs if large tx |
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Acid decal for fzn sectn |
Formol saline containing 15% sucrose |
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Acid decal for fzn sectn |
Or Phosphate-buffered saline with 15-20% sucrose |
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Decal in EDTA |
Placed directly to alcohol |
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Molliflex, 2% HCl, or 1% HCl in 70% alcohol |
Examples of tissue softeners |