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6 Cards in this Set
- Front
- Back
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Gastrulation |
Embryo undergoes rearrangement of its cells, sets aside its germ cells and forms: - primary germ layers - axes anterior/posterior dorsal/ventral |
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The 4 tests of pluripotency |
1. In vitro differenetiation 2. In vivo teratoma formation/differentiate 3. In vivo germline chimerism 4. In vivo tetraploid complementation |
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Pluripotency test 1 |
In vitro differentiation Least stringent does not test function. Cells differentiate spontaneously into the 3 germ layers ectoderm, mesoderm, endoderm |
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Pluripotency test 2 |
In vivo teratoma formation. In skid mouse. Differentiate spontaneously into the 3 germ layers. Does not test ability to promote normal development. |
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Pluripotency test 3 |
In vivo germline chimerism. Black mouse ESC added to white mouse blastocyst. Offspring black and white. Tests for germline competency. May not be accurate due to epigenetic effects. |
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Pluripotency test 4 |
In vivo tetraploid complementation. 4n blastocyst has ESC added these cells form the embryo.Tests for offspring competency. . Tests for offspring competency. |
