Bmsc 220 -Ch. 5&6

Front 1

True or false Cis-acting DNA Sequence Elements

regulate the expression of eukaryotic genes.

Back 1

True, Reference slide 15/73, they are further divided into promoter and enchancer elements

Front 2

Explain how proteins are like "dimmer switches"

Back 2

-combination of transcription factors interacting at a specific gene’s promoter/enhancer sequences

- combination specifies the amount of transcriptional initiation by RNA pol II at that gene in a particular cell type. (when, where, how much)

- very fine function because of these combinations.

Front 3

What is the difference between gene-specific and general transcription factors?

Back 3

General:

involved in transcription from all polymerase II promoters. They have part of the basic (or general) transcription machinery-involved in formation of the transcription initiation complex (also known as preinitiation complex).

Gene-specific:

Not part of the general machinery.

- bind to promoter and enhancer elements of one or a small number of different genes, and direct activity of the general transcription factors

Front 4

Which of these components is NOT part of the preinitiation complex?

TAF,TBP, TFIIB, TIIF, TFIIH, H1

Back 4

H1 is part of the Chromatosome

Front 5

True or false, the transcription initiation complex is responsible for the initiation and the rate of General transcription.

Back 5

False- the transcription initiation complex is NOT responsible for the rate
How ever it is responsible for initiation in General transcription.

Front 6

True or false, Steroid Hormone proteins (contain a helix-turn-helix DNA binding domain) play critical roles in the regulation of gene expression during embryonic development.

Back 6

False, this is the definition for Homeodomain proteins,

Steroid Hormone Receptors:

contain a zinc finger domain) are transcription factors that regulate gene transcription in response to hormones such as estrogen and testosterone.

- therefore they are not present in embryonic development

Front 7

Transcription factors contain separate DNA-binding and activation/repression domains.

What are 2 general mechanisms of action for Transcription Factors.

Back 7

2 general mechanisms of action

1. Interact with mediators and general transcription factors

2. Interact with coactivators, which modify chromatin structure.
slide 34/73

Front 8

Gene expression in eukaryotic cells is regulated by repressors as well as by transcriptional activators.

True or False,
They essentially only
- Repress
- Interact

with other transcription molecules

Back 8

False, they also function by
Blocking binding of transcriptional activator to DNA sequence element

Full function below:
- Repressing mediator activity
- Interaction with co-repressors, which serve to modify chromatin structure into a non-permissive state

Front 9

__________________________is the modification of histones by the addition of acetyl groups to specific lysine residues in histone tails.

Back 9

Histone acetylation

Front 10

True or False:A 7-methylguanosine cap is added last during the modification of the 3' end of a transcript, often after transcription has ceased.

Back 10

False:A 7-methylguanosine cap is first added during the modification of the 5'end of a transcript, often before transcription has ceased.

Front 11

Find the error in the message:

One snRNA complex forms the splicesome, and carry out the splicing reactions

Back 11

Multiple snRNP complex forms the splicesome, and carry out the splicing reactions, and what a snRNP is

Front 12

5.8S, 18S, and 28S rRNA
makes up the majority of cellular RNA (over 80% of total cellular RNA)

True or False

- these ribosomes are spliced in the cell

Back 12

False, they are not