Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
16 Cards in this Set
- Front
- Back
what evidence does genetic mapping use to estimate the proximity of two loci on a given chromosome?
|
frequency of crossing over
|
|
if two loci are close together, are they more or less likely to recombine than if they are far apart?
|
less
|
|
how can recombination frequencies be estimated for genetic mapping?
|
by observing the transmission of genes in informative families. Number of recombinants is counted and % of recombinants calculated in the progeny of an informative individual
|
|
what is the unit set equal to 1% recombination frequency?
|
a centimorgan
|
|
how far is a centimorgan in molecular terms?
|
1 million base pairs of DNA
|
|
which statistical method is used to test whether a set of linkage data from family studies indicates linkage between two loci or not?
|
LOD score
|
|
is a LOD score of +3 better worse than an LOD score of -2?
|
better; the higher, the more likely the linkage data is real
|
|
how does one establish linkage between disease locus and a marker? What is this process called?
|
select a number of markers systematically from all over the genome and test each one for linkage; a genome scan
|
|
which markers are of particular help in genome scanning?
|
microsatellite repeats
|
|
what is the difference between linkage and association?
|
linkage refers to location of genes closely on the same chromosome while association refers to co-occurrence of different phenotypes more often than expected
|
|
what is a very common method used to map genes to chromosomes?
|
FISH
|
|
on average, useful DNA polymorphisms can be found at which intervals?
|
less than 1 centimorgan
|
|
in which type of vector were DNA fragments inserted for the human genome project? Which restriction enzyme was used?
|
bacterial artificial chromosomes; HindIII
|
|
what is functional cloning?
|
ID of a disease gene based on knowledge of the protein involved in the disease, without knowing the chromosomal location of the gene
|
|
what is candidate gene approach?
|
when an educated guess is made about a protein involved in a disease and the DNA coding for any such protein is then tested for mutations in affected individuals
|
|
what must be known in positional candidate approach?
|
the knowledge of the correct chromosomal subregion of the gene of interest.
|