Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
44 Cards in this Set
- Front
- Back
|
ECM fibers include |
collagen and elastic fibers |
|
|
Elastic Fibers: -fncn: -stain: -appearance once stained: |
-lets tissue be stretched -orcein -turn black/dark brown |
|
|
Elastic fibers: Structure: -___ core -with ____ |
-elastin core -fibrillin microfibrils |
|
|
Collagen fncn: -flexible but not -made of |
-gives TENSILE STRENGTH -flex but not elastic -made of fibrillar 1 2 3 and sheet forming 4 |
|
|
Assembly of collagen: -all collagens get to __ stage, but not all get to __ |
-tropocollagen then fibrils -not all become fibers or fiber bundles |
|
|
what has periodicity what microscope shows it |
collagen fibrils TEM |
|
|
TYPE I Collagen: -found in: -fncn: __ strength -will make fiber bundles |
-sclera, dentin, organ capsule, scar tissue, fascia, bone, ligaments, joint capsules,
-tensile |
|
|
what is most abundant collagen? |
type1 |
|
|
Type 1 -gross appearance: -light microscope: ---H and E it goes: ---trichrome is goes: |
-white! -pink bundles -green or blue in trichrome stain |
|
|
type 1 defect: - |
brittle bone disease |
|
|
type II collagen (hyaline): -makes fibers, but doesnt bundle! -found in: -fncn: -type II defect: |
-cartilage -resist pressure -growth plates defective=achondroplasia (dwarf) |
|
|
Type III Collagen: Reticular fibers -fibrillar collagen that remains as __ (thus will show__) -reticular fibers are ___ so have sugar attached -stain for reticular fibers: __ will show up __ -can also be stained with __ |
-as fibrils, periodicity -heavily glycosylated! -silver show up black -also stained with PAS (shows glyc) |
|
|
Type III collagen is found in:_ fncn: give support to _ and _ |
skin bld vessels and internal organs |
|
|
type III defect: -hypertensible skin, easy __ -early__ due to __ -called __ |
bruising -death due to rupture of bld vessels and organs -Ehlers-Danlos syndrome |
|
|
Type IV: -found in basal lamina of __ and external lamina of __ -fncn: support __, part of __ |
epithelium, muscle and nerves -support epithelial cells, part of filtration barrier |
|
|
ECM ground substances include: |
-GAGs -proteoglycans and proteoglycan aggregates -multi adhesive glycoproteins |
|
|
what is ECM ground substance |
space between cells and fibers clear, high water content lost with staining so looks empty |
|
|
proteoglycans and proteoglycan aggregates: -are __ -highly __ charge, so attracts __ |
sulfated GAGs that attach to them -highly negative charge, attracts water |
|
|
LM appearance for GAGs and proteos: PAS for them |
-blue purple -pink |
|
|
CT cells 2 groups: - - |
fixed )resident) cells transient cells |
|
|
Fixed resident cells: - Transient cells: - |
-fibroblasts, macrophage, mast, adipocytes -WBC, plasma cells |
|
|
Fixed - Fibroblasts -__ CT cell -makes and secretes ___ including__ -always seen where? |
-principal -all ECM components like fibers, collagen fibers, ground substances, elastic fibers -seen close to collagen fibers! |
|
|
Fibroblasts in LM: -__ cell -stain _ bc _ In TEM: -seen next to __ Regulated or constitutive? |
-look elongated! -stains purple bc basophilic -seen next to collagen fibers longitudinal and cross section -Constituvtive |
|
|
Fibroblast vs firbrocyte Fibroblast: very pink everything -lots of cells very __ -cell structure shows _ -happens during__ Fibrocyte: white background, purple cells -less _, more spread -shows less activity -is just for __ |
-close together -intense synth actibvity -growth or repair -less cells -just for ECM maintenance |
|
|
Fixed resident cells - Macrophages: -arise from __ -these come from bld into CT and differentiate -is part of __ |
come from monocytes (WBC) -part of MONONUCLEAR PHAGOCYTE SYSTEM OF CELLS |
|
|
Macrophage fncn: |
-phagocytosis -antigen presentation -secreation |
|
|
macrophage LM: -large _ -__ nucleus -MANY __ TEM: -irregular border |
large cells eccentric nucleus many LYSOSOMES |
|
|
Fixed cells - Mast LM: -nucleus is__ -cytoplasm is __ -shows METACHROMASIA which is TEM: -nucleus is __ -cytoplasm is |
-round -filled with dark secr granules -METACHROM is when color varies in basic stain -nulceus is center -e dense secr granules |
|
|
Mast fncn secretory granuels contain: -heparin; -histamine; -WBC chemotactic factors; |
-anticoagluant -helps vasc perm, smooth muscle contract, makes mucous -attracts WBC to region |
|
|
Mast found in |
skin respiratory GI |
|
|
Fixed cell - Unilocular adipocytes (most common type of fat cell) -fncn: make and store _ -secretes __ Stain: |
fat hormones, growth factors, cytokines Stain: -osmic acid and sudan red |
|
|
Adipocytes -large or small? -nucleus is _ -fat stored as __ |
-large cells -eccentric (not in centeR) -single droplet |
|
|
Transient connective tissue cells WBC fncn |
injury and inflamm host defense |
|
|
Transient - Plasma -nucleus is__ -heterochromatin distribution makes __ -fncn: |
eccentric clockface makes antibodies (proteins therefore golgi and rER) |
|
|
plasma cell LM: - TEM: - Secreation is regulated or consitivute? |
LM: basophilic, negative golgi TEM: organelles? -consitutive |
|
|
Connective Tissue Classifications 2 categories: |
embryonic CT tissues connective tissue proper |
|
|
Embryonic CT: - - |
mesenchyme mucoid |
|
|
Connective tissues proper - - - |
-loose -dense (irregular and regualr) -reticular tissues |
|
|
Embryonic CT Mesenchyme: -__ shaped with _ nuclei -few _, mostly _ MucoidCT -__ and lots of ground substance -found in _ |
-spindle, oval nuclei -few fibers, mostly ground substances -fibroblasts -found in umbilical cord |
|
|
CT proper Loost CT: -lots of cells and ground substances -found __ -primary site of __ |
beneath epithelim site of immune response and inflamm rns |
|
|
CT proper Dense CT: -lots of __ -not much __ (thats why has compact appearance) -can be reg or ireg (will affect _) |
bundle fibers not much ground substances will affect direction of tensile strength |
|
|
Dense Irregular -collagen aligned __ -found in __ |
-in many directions -found in skin and organ capsules |
|
|
Dense Regular -collagen bundles align__ -found in |
-all in one direction -found in ligaments and tendons |
|
|
CT proper - Reticular tissue -reticular fiber ssecreted by __ -they make netword where __ -stroma of __ -found in : |
-fibroblasts -fluid and cells can pass thru continuiously -stroma of filtering organs -found in bone marrow, liver, panc, adrenal glands, lymph organs |
