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11 Cards in this Set
- Front
- Back
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non culturable strains |
strains that cannot be grown in a laboratory bc the appropriate growth conditions have not been discovered yet
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most microbes on earth are |
non culturable |
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16S rRNA |
RNA that is structural compoent of the small subunit of the ribosome |
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Which sequences are more highly conserved? |
double stranded regions tend to be more highly conserved than sequences in the single stranded regions |
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BLAST SEARCH |
t a database containing thed 16S rRNA gene sequences from all known species with THIS TOOL |
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why is pCR needed |
to amply DNA to actually analyze |
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BLAST steps |
1. genomic DNA isolated from clinical sample 2. PCR is used to amply 16S rRNA gene from this sample 3. DNA is sequenced 4. sequence is matched against the NCBI |
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how to show 16SrRNA legit ness? |
bacteria DNA |
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why is the 16S rRNA sequence commonly used for identifying bacteria? |
best thing to identify |
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rDNA |
nucleoid |
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rRNA |
ribosome subunit coded from rDNA |
