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24 Cards in this Set

  • Front
  • Back

Monosaccharide

Single sugar unit


Same number of C and O


Glucose, ribose, galactose and fructose

Number of carbon in glucose

6 so hexose monosaccharide

Alpha glucose

At carbon 1, the OH group points below the plane of the ring

Alpha glucose + alpha glucose


The bond

Maltose + water


1,4 glycosidic bond

Beta glucose

At carbon 1, the OH group points above the plane of the ring

Condensation reaction

Join 2 monosaccharide to form disaccharide and water

Hydrolysis

Add water to break bond in disaccharide to form monosaccharide

Glucose + fructose


Bond

Sucrose


Alpha 1-2 glycosidic bond or 1-6 glycosidic bond

Pentose monosaccharide

5 carbon


Ribose - contain oxygen in carbon 2


Deoxyribose - no oxygen

Galactose + beta glucose

Lactose


1,4 glycosidic bond

Starch structure and features

Alpha helical structure so compact


Good for storage


Plant storage sugar


Made of straight chain amylose and branched amylopectin

Amylose consist of


Feature

lots of alpha glucose joined together only by 1,4 glycosidic bond


compact and less soluble due to helix and hydrogen bonding

Amylopectin made by

1,4 glycosidic bond and some 1,6 glycosidic bond

Storage in animals

Glycogen

Similarities of glycogen and amylopectin

Both made of alpha glucose


Both have 1,4 glycosidic bond and 1,6 glycosidic bond


Both are energy storage carbohydrates


Both does not affect water potential as they are insoluble

Differences of amylopectin and glycogen

Glycogen has shorter branches


Glycogen is more branched so more compact and less space is needed for it to be stored


Glycogen has more free ends so glucose can be added and removed quicker

What is cellulose made of ?


The bonds


The arrangement and features

Beta glucose join together


Beta 1,4 glycosidic bond


Alternate beta glucose are flipped


6 OH group from hydrogen bonds with a strand above and below to form fibres - provide mechanical strength and insoluble

Test for reducing sugars

Benedict test

The explanation for Benedict test

Benedict solution (blue) contain Cu2+ and reducing sugar add electron to Cu2+ to form Cu+ turning it red

The procedure for Benedict test

Add food sample in boiling tube in liquid form


Add equal volume of Benedict reagent


Heat mixture in boiling water bath go 5 minutes

Why can’t non reducing sugar reduce Cu2+?

Because the chemical needed for reduction is involved in glycosidic bond so it must be hydrolysed to expose reducing groups

Procedure test for non reducing sugar

Add 2cm3 of food sample to 2 cm3 of dilute HCl


Place in gently boiling water bath for 5 minutes


Slowly add sodium hydrogen carbonate until neutralise


Retest with Benedict solution

Determine concentration of glucose solution procedure

Benedict’s test to a range of known glucose concentration solution and one unknown


Filter solution to remove precipitate


Red filter place in colorimeter


Colorimeter calibrated using distilled water zeroing it


Measure percentage transmission and draw calibration graph


Determine concentration that correspond to percentage transmission on graph

Test for starch

Few of iodine solution


Positive - yellow/brown to blue black