Mid Micro Genetics

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SCIENTIFIC TERM

Segment of DNA

MCQSum of genes

Study of inheritance

Unit of heredity

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Gene

Genome

Genetics

Gene

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Genetic Info Carried on

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Bacterial Chromosome

Plasmid

Transposons

Bacteriophages

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Plasmid : MCQ F45

-Type of chromosome

-MCQ EXAM Replication.

-MCQ Necessity

-MCQ Size Compared to Chromosome

-MCQ Cell Prop determined by Plasmid

-Prop :

1)Self transfer :

- MCQ EXAM plasmids of Gm +ve or - ve

- MCQ by Production of w which carry which?? genes

2)Recombination : MCQ F4555555

-is ⬆️ by w consists of

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Extra

Self (autonomosly)

Not necessary (Dispensable)

Smaller

-Drug Resistance (R plasmid)

-Virulence

-Bacteriocin Production

-Antibiotics Production

-Sex pilus Production

1)-ve.. Sex pili....tra... Conjugation

2)Episome.... Plasmid + Chromosome

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-MCQ EXAM Which Plasmids (Gm +ve or - ve) lack self Transfer w lack tra genes for sex pilli w other name of this Plasmids

- Replication of Chromosome by

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+ve... Non Conjugative

-Semi conservative method(transverse binary fission)

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MCQ F45

Cell Prop determined by Plasmid :

1)MCQ F45 Drug Resistance مهمة :

-Carried on w trans by

-Ex

2)Virulence مهمة

-Ex : Gene coding for Production of

3)Bacteriocin Production

-Like

4)Antibiotics Production

5)MCQ Sex pilus Production

-That carry w Process

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R-Plasmid .. Generalized Transduction

B Lactamase

Exotoxin

Antibiotic

Tra genes... Conjugation

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Transposons :

-MCQ Other name

-Small mobile Move from مهمة فشخ

-MCQ Characteristics same to Plasmid -MCQ F45 Charachertistics diff from Plasmid

-MCQ Characteristics other

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Jumping Genes

One Location to Another in :

-نفس الchromosome

-من Plasmid لPlasmid

-من Plasmid ل Chromosome

Transmissible

Dispensable

not self Replicate عشان صغننة

-Resistance to Antibiotics or Toxin Production (Virulence) مهمة

- Cause mutations

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Bacteriophage :

-MCQ Are

-MCQ F4555 اهم كلمة Show ⬆️ or ⬇️ host specificity Structure : Head w Tail

-Interaction between Bacteriophage w Bacteria Types (Cycles) مهمة فشخ

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Viruses

⬆️..

Bacteriolysis (Lytic cycle)

Lysogeny

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Bacteriolysis :

-MCQ Other name

-MCQ F45 Responsible for which Type of transmission

-Release

Lysogeny :

-MCQ F45 other name

-MCQ F45 Responsible for which Type of transmission

-MCQ F455 is it End in lysis

-MCQ F45 When bind to chromosome so called

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Lytic (Vurulent Phage)

Generalized Transduction

-Many copies of Phage

نسخ كتيرة

Temperate Phage الكسلان

Specialized Transduction

No

Prophage

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-MCQ Importance of Bacteriophage

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-Gene Transfer (Transduction)

-Lysogeny conversion مهمة فشخخخ

- Phage typing

-Genetic Engineering

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MCQ EXAM

Bacterial Variation :

-Change in

-Types

-Reversibility

-Hereditary

-Ex

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Environment

Bacterial Genes

Phenotypic شكل

Genotypic

Reversible

Irreversible

Non heritable

Heritable

-Spore w vegetation and S-R Smooth w Rough Colony

- Mutation (no gene Transfer) w Gene Transfer

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Mutation

-No

May be : spontaneous w Induced

-Spontaneous due to

-Eff

- MCQ Use

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-Gene Transfer

-Replication error

-Mutation of low Virulence

-Production of Vaccine w Antibiotic

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Methods of gene Transfer

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Transformation

Transduction

Conjugation

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MCQ F45

Transformation

-Exam : Keyword اهم كلمة

-MCQ F45 Most bacteria not naturally (Natural is uncommon) due to

-MCQ Must be between

-Induced ex

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-Free (Pure or naked) DNA

-Restriction Endonuclease Enzyme.. Digests any foreign DNA

-Donor w recipient

-CaCl2 or Heat Shock

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لازم بكتيريا نفس النوع و يبقي فيه خرم

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MCQ

Conjugation MCQ

- EXAM By Direct Contact through w this through which controlled by

-This Process controlled by

-G - ve or +ve

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Sex pili... Tra genes

F Plasmid

-ve

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MCQ

Transduction..

By..

Types

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Bacteriophage

Generalized

Specialized

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MCQ

Generalized :

-During

-Mech

-EX

Specialized :

-During

-Mech

-How many DNA

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Lytic phage

خد حتة من DNA

Mispackage

B Lactamase (ely fe el drug resistance bta3t el Plasmid)

Temperate (Lysogeny)

Incorrect separation of prophage

بقي شوية Phage و شوية bacteria

Part

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MCQ مش مهمة اوي

Genetic Engineering...

-MCQ F45 Modification of which Type of Variation

-By

-DNA Molecule containing new gene.. Called

MCQ F45 : Insertion or fragment of DNA into (Plasmid or Phage) w Propagation... This called

-DNA extraction from

-Both DNA treated by enzymes what

-Uses

-MCQ F45 Short seq of SS DNA or RNA Labeled with radioactive isotope lr enzyme = detect

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Genotypic

نحط new genes

-Recombinant DNA

Cloning

-Eukaryotic w Vector(Plasmid or Phage)

-Restriction Endonuclease Enzyme

-DNA ligase enzyme

-Production of Hormones w vaccines

-Diagnosis by use of NA probe

-TTT by gene therapy

-Chromosomal mapping w DNA Sequencing

Complementary Strand

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Essay

Define w Ex (of toxins Phage control them)

Lysogenic conversion

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Any change in bacterial characters after entry of prophage

Erythrogenic toxin of St.pyogenes w Diphtheria Toxin

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الErythrogenic دي تعمل Scarlet Fever

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MCQ

Gene Transfer Mostly by

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Transduction

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مهم فشخ

B Lactamase

-Carried by

-Trans by

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-R Plasmid

-G Transduction