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70 Cards in this Set
- Front
- Back
Used in serotyping by swelling |
Capsule |
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To demonstrate capsules, grow culture on media containing |
Milk and serum |
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Quellung test depends on the antigenic specificity of the |
Capsule |
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Dark staining granules |
Metachromatic |
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Organisms that vary in shape and size |
Pleomorphic |
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Growth ceases because nutrients are exhausted or toxic metabolic products have accumulated |
Stationary phase |
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Bacteria that grow in the absence of atmospheric oxygen; obtain oxygen from oxygen-containing compounds |
Anaerobes |
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Temperature at which bacteria grow best |
Optimum |
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Slimy colony on blood agar medium |
Organism has capsule |
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Zone of alpha-hemolysis surrounded by zone of beta-hemolysis after refrigeration |
Alpha-prime |
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Fractional sterilization sequence |
Heat - incubate - heat |
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Most effective method of sterilization |
Autoclave |
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QC for dry heat oven |
Bacillus subtilis |
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Filter that can give 100% sterility |
Millipore (0.22um) |
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Media that cannot be heated can be sterilized by |
Filtration |
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Iodophore composition |
Iodine & detergent |
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Easily inactivates quarternary ammonium disinfectants |
Organic material |
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Process or treatment that renders medical devices, instrument, or environmental surface safe to handle |
Decontamination |
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Gram positive bacteria stain |
Purple |
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Mordant used in Ziehl-Neelsen acid-fast stain |
Heat |
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Fixative of smear for acid-fast stain |
Slide warmer at 65 degrees celcius for 2 hours |
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Acid fast bacteria appear what color |
Red |
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Best stain for acid-fast organisms in tissue |
Kinyoun |
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Decolorizer for Ziehl-Neelsen staining method |
Alcohol + Hydrochloric acid (Acid alcohol) |
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India ink is an example of |
Negative stain |
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Peptococcus: gram positive or gram negative? |
Gram positive |
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Campylobacter: aerobe or anaerobe? |
Aerobe |
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Can neutralize antimicrobials |
Thiol broth |
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Used to remove antimicrobials before culturing |
Antimicrobial removal device |
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Anticoagulant in blood cultures that prevents phagocytosis and neutralizes bactericidal effect of human serum. |
Sodium Polyanethol Sulfonate (SPS) |
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Most common pathogen in throat cultures |
Group A Streptococcus |
|
Recommended specimen for H influenzae, Neisseria & B pertussis |
Nasopharyngeal swab |
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Recommended for culture of beta-hemolytic streptococci for fluorescence microscopy |
Todd-Hewitt broth |
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Phenylethyl Alcohol is used to |
Inhibit gram negative bacteria |
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Urine specimen preferred for urine cultures |
First morning urine |
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Indicates vaginal or urethral contamination |
Many squamous epithelial cells |
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Smears of cerebrospinal fluid are stained with |
Gram stain and India ink stain |
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Sensitive and fast test for cultures isolated from cerebrospinal fluid |
Latex agglutination tests |
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Staphylococcal protein A coated with antiserum is used in |
Coagglutination test |
|
Sheep blood for blood agar plates eliminates |
Beta-hemolytic Haemophilus |
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Positive coagulase test |
Clotting of plasma |
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Plasma used for tube coagulase test |
Rabbit plasma |
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Nonhemolytic, catalase positive, coagulase negative, gram positive cocci |
Staphylococcus epidermidis |
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Test to differentiate S aureus from S epidermidis |
Coagulase test |
|
Staphylococci which causes urinary tract infection in young females |
S saprophyticus |
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Medium for isolation of Staphylococcus aureus from stool |
Mannitol Salt Agar (7.5% salt concentration) |
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Most common cause of bacterial food poisoning |
S aureus |
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Gram positive cocci that ferment glucose |
Staphylococci |
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Oxygen stable, non-antigenic hemolysin |
Streptolysin S |
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Hemolysin destroyed by oxygen |
Streptolysin O |
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Bacitracin susceptible |
S pyogenes (Group A beta-hemolytic streptococci) |
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Optochin disk susceptible |
S pneumoniae |
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Optochin disk resistant |
S viridans (alpha-hemolytic streptococci) |
|
Hippurate hydrolysis positive, CAMP test positive |
S agalactiae |
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Bile-esculin positive |
Enterococcus |
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Positive reaction to CAMP test |
Arrowhead hemolysis |
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Capsular swelling due to antigen-antibody reaction is positive for |
Quellung reaction |
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Confirmatory tests for streptococci |
Phadebac test Fluorescent antibody test Lancefield precipitin test |
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Units of Bacitracin disks for differentiation of group A beta-hemolytic streptococci |
0.02-0.04 units |
|
Causes Scarlet fever |
B pyogenes |
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Major pathogen of the newborn |
S agalactiae |
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Causes "strep throat" |
S pyogenes |
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Subacute bacterial endocarditis |
S viridans |
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Positive result for both Optochin and Bacitracin |
Growth inhibition |
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Used to differentiate Group D streptococci from other streptococci |
Bile-esculin test |
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Used to differentiate Group A from Group B streptococci |
Hippurate hydrolysis test |
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Bacitracin resistant, hippurate hydrolysis positive, Bile-esculin positive, beta-hemolytic Streptococcus, grows in 6.5% NaCl |
Enterococcus |
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Gram negative, coffee bean shaed diplococci with adjacent flattened sides |
Neisseria |
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Diagnosis of gonorrhoeae in males |
Positive urethral smear Symptoms History |
|
Appropriate specimens for culturing Neisseria gonorrhoeae |
Eyes Rectum Oral cavity |