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68 Cards in this Set
- Front
- Back
where is the fire extinguisher?
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to the left of teh entrance door
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Where is the spill control station
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in the cabinet midway down the entrance door wall
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where is the eye station
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on the back wall to the right
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where is the shower
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down the hall
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what genus has a scolex, suckers, hooks and proglottids
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Taenia
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what genus has oral suckers, ovaries and testes
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Fasciola
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what genus has larvae in skeletal muscle cells and nurse cells
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trichinella
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what genus has cutting plate
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necator
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what genus has a nucleus and pseudopods
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amoeba
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what genus has cilia?
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Paramecium
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what's the feature of a microscope which allows object viewed to remain in approximate focus when objective is changed
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parfocal
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what is the vertical depth of specimen in focus
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the depth of field
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what is the area of slide being observed
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Field of view
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what genus has vegetative cells and budding cells
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candida
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how to adjust the amount of light that shines on a microscope slide
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adjust the iris diaphragm
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what are the two main structures of penicillum and aspergillus
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hyphae and conidia
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identify this elevation
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convex
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identify this elevation
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umbonate
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identify this elevation
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plateau
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identify the flat elevation
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flat
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identify the raised elevation
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raised
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identify this elevation
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raised, spreading edge
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identify this elevation
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flat, raised margin
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identify this elevation
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growth into medium
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identify the whole colony descriptor
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round
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identify the irregular whole colony descriptor
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irregular
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identify the whole colony descriptor
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filamentous
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identify the whole colony descriptor
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rhizoid
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identify the margin descriptor
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smooth, entire
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identify the rhizoid margin descriptor
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rhizoid
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identify the margin descriptor
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irregular
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identify the lobate margin descriptor
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lobate
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identify the margin descriptor
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filamentous
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describe simple stain
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only one stain is used, no decolorization ste;
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describe the order of the Gram stain
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First use Crystal violet stain (rinse with distilled water)
then fix with iodine stain (rinse with D.I.) Then decolorize with ethanol Then counter stain with safranin (Rinse with D.I.) |
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Describe the steps for the Ziehl-Neelsen (ZN) acid-fast stain
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apply ZN carbolfuchsin stain - steam for 5 minutes (rinse w/d.i.)
decolorize with acid-alcohol (rinse w/d.i.) counterstain with Methylene Blue then rinse with d.i. |
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what differentiates candida from other fungi?
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candida does not have hyphae
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what is the liquid medium used to test for aerotolerance?
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fluid thyoglycollate medium
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if you see growth mostly at the top but also throughout a FTM sample tube, what is the aerotolerance category of the organism?
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facultative anaerobe
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if you see growth only at the very top of a FTM sample tube, what is the aerotolerance category of the organism?
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obligate aerobe
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if you see growth only at the bottom of a FTM sample tube, what is the aerotolerance category of the organism?
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obligate anaerobe
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describe aerotolerant anaerobic bacteria
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not affected by oxygen and are evenly spread out along the test tube.
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what is the genus of the org that has zygospores
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rhizopus
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what is the term for arrangement of long chains of rod-shaped bacteria
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streptobacillus
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what is the term for arrangement of long chains of sphere-shaped bacteria?
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streptococcus
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term that describes bacteria as being everywher
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ubiquity
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name the media we used for the ubiquity cultures
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sheeps blood agar
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during the Gram stain technique, the iodine stain acts a s a _________ to the crystal violet stain
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trapping agent
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for what type of bacterium is the acid-fast stain technique used
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mycobacterium
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describe why the acid-fast stain is used for mycobacterium
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b/c mycobacterium hve a waxy, hydrophobic layer of mycolic acids on the outside of the cell wall protecting it. This layer will not wash away in acid, so if treated with acid-alcohol and it doesn't wash away, you know it's an acid-fast reaction, positive for mycolic acids of mycobacterium
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===
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===
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Name the 5 stain techniques we've studied
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simple
Gram ZN Acid-Fast Capsule endospore |
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describe the endospore stain
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requires a 10 minute steaming period to force the Malachite Green into the endospore.
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what is the counter stain used in endospore staining
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Safranin
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what is the counter stain used in the ZN Acid Fast stain
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Methylene Blue
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what color is positive and negative for acid fast results?
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pink is positive for acid fast
blue is negative |
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how deos the capsule staining technique work?
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capsules composes of polysaccharides or polyeptides that repel most stains. Acidic stain first stains the background and then a basic stain colorizes the cell proper, leaving the capsule unstained and appears as a white halo
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what are the two stains used in the Gram stain
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Crystal Violet
Safranin |
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what are the two stains used in the acid fast ZN?
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Ziehl's carbolfuchsin stain
Methylene Blue |
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what are the two stains used in the endospore stain?
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Malachite Green
Safranin |
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what are the two stains used in the capsule stain?
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Congo red
Maneval's stain |
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how do you handle a spill
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cover the spill with paper towels, soak the towels with disinfectant, allow to stand for 20 minutes. report spill to instructor. dispose of towels in biohazard barrel
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name a disease caused by an acid-fast organism
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leprosy
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name a disease that is caused by a capsulated organism
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anthrax
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name the genus of on organism that forms endospores
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Bacillus
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name a disease caused by an endospore former
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Botulism
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packets of cocci are called
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(sarcinae)
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what is the next step of transfer after you have removed the screw cap of a culture tube?
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flame the open end of the culture tube by passing it through the flame 2-3 times.
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