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50 Cards in this Set
- Front
- Back
Identify the two types of hemolysis that can occur on blood agar plates.
Which is these is worse? |
Alphahemolysis
Betahemolysis (worse) |
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Describe what alphahemolysis looks like on a blood agar plate.
What does it represent? |
It looks like a dark green halo. It represents only a partial destruction of blood cells.
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Describe what betahemolysis looks like on a blood agar plate.
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There is NO red color.
This represents complete destruction of blood cells. Tip: Beta starts with the letter B and so does BAD! Betahemolysis is bad b/c it destroys blood cells. |
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If your test tube was cloudy when shaken up, what did that mean?
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Bacteria is present.
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pure culture
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a laboratory culture containing a single species of organism, usually derived from a mixed culture
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mixed culture
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a laboratory culture containing many species of organisms
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Identify the 4 bacteria in the mixed culture.
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Micrococcus luteus
Serratia marcescens Escheria coli Chromobacterium violaceum |
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Micrococcus luteus
-color -gram -common location |
Yellow
Gram (+) Skin, soil, hair |
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Serratia marcescens
-color -gram -common disease |
Red
Gram (-) Bladder infection |
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Escheria coli
-color -gram -common location |
White
Gram (-) Colon |
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Chromobacterium violaceum
-color -gram -common location -common disease |
Purple
Gram (-) Water, soil Pus-forming infections |
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What streak method is used in lab and why?
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We use the quadrant streak method because we want to isolate a single colony in order to form a pure culture.
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What is the plant cell wall made of?
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Cellulose
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Are fungi unicellular or multicellular?
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They are both unicellular AND multicellular. Mushrooms are filamentous.
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Identify the 3 types of fungi and whether they are unicellular, multicellular, or filamentous.
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Yeasts (unicellular)
Molds (filamentous) Mushrooms (multicellular) |
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Identify the ideal conditions for fungi.
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On land in 20-35 C° at a pH 5
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Identify 3 functions of fungi.
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Decompose organic material
Release CO2 into the air Release nitrogen compounds into the soil |
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What do all fungi have in their cell walls?
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Chitin
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Do any fungi have flagella?
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None do, except for chytrids in their reproductive form.
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Identify the 3 ways that fungi can reproduce.
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Asexually by mitosis
Budding in yeast Hyphae in molds |
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Identify 2 ways that fungi can cause harm.
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Cause plant disease such as Dutch elm disease and wheat rust
Cause allergic reactions in humans due to toxins produced by aspergillis |
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Identify 2 ways that fungi can do good.
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Yeast are used in wine and bread-making.
Some fungi produce penicillin. |
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Is negative staining acidic or non-acidic?
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Acidic
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Does negative staining cause a negative charge or a positive charge?
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Negative charge
Tip: Duh?! |
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Identify the 2 most commonly used negative staining products.
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Nigrosin
India ink |
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Why does a bacteria's membrane repel the negative stain ink?
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They repel each other because they BOTH have a negative charge.
The bacteria's membrane (phospholipid) has a negative charge. The negative stain has a negative charge. |
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In actuality, what does the negative stain color?
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Negative stains color the area AROUND the bacteria, but not the bacteria itself.
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In what way is negative staining useful?
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It is useful in examining the shape or morphology of bacteria.
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#1
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Coccus
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#2
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Diplococci
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#3
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Streptococci
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#4
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Tetrads
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#5
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Staphylococci
Note: Cluster |
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#6
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Sarcina
Tip: Cube of 8 |
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#1
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Bacillus
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#1
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Bacillus
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#2
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Streptobacilli
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#3
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Coccobacillus
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A
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Bacillus
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B
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Streptococci
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C
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Staphylococci
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D
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Diplococci
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E
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Spirillum
Note: Loose spiral |
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F
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Vibrio
Tip: It's like a comma. |
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#1
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Vibrio
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#2
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Spirillum
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#3
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Spirochete
Note: Tight spiral |
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What shape are pleomorphic bacteria?
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They have no particular shape.
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Identify the arrangement of this bacteria.
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Palisade
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Identify the shape of this bacteria.
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Diplobacilli
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