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13 Cards in this Set
- Front
- Back
What is recombinant DNA? (How many sources of genetic material does it consist of?)
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DNA material consisting of genetic material from 2 or more sources
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How many chromosomes do bacteria have?
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One
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What is a plasmid?
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A circular piece of DNA separate from the main chromosome in bacteria
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In recombinant DNA technology, plasmids can be used as _____
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Vectors
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Vectors move what from one cell to another?
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Genes
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What do you do to the plasmid in the first step of creating recombinant DNA?
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You isolate the plasmid from the bacteria
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Next, human ___ must be isolated from a cell
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DNA
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What is used to cut DNA containing the gene of interest from a chromosome?
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Restriction enzyme
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The cutting produces nitrogenous base overhangs and underhangs called _____
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sticky ends
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Nitrogenous bases _____ bond with each other
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Hydrogen
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To more permanently attach the gene of interest onto our plasmid, we use an enzyme called _____
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Lygase
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Bacteria are treated with _____ _____ to alter their cell wall and allow some to take up the plasmid
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Calcium chloride
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What are proteins that dissolve blood clots?
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Anticoagulants
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