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10 Cards in this Set
- Front
- Back
What is the Henderson-Hasselbach equation?
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pH= pka+log([B]/[A])
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What are the steps to charge and mass balance?
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Set positive species equal to negative species
Set initial concentration of acid or base equal to the conc. of its conjugate Use Kw=1e14=[H3O+][OH-] Use quadratic formula to solve for unknown species |
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What is the quadratic formula?
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(-b+or-(b^2-4ac)^(1/2))/2a
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What are the draw backs of the biuret method?
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Tris and ammonia interfere with the assay because they react with copper
The assay is insensitive |
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What are the draw backs of the Lowry method?
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defeated by a variety of contaminants
low protein conc. needed precise timing needed |
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What are the draw backs of the BCA method?
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defeated by carbohydrates
color continues to develop over time |
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What are the draw backs of the Bradford method?
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defeated by high conc. of detergents
stains cuvettes |
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When is gel filtration used?
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to separate molecules according to Stoke's radius.
When the desired molecule is much larger or smaller than the others |
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When is affinity chromatography used?
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To seperate proteins, nucleic acids, antibodies, and other biomolecules
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When is ion-exchange chromatography used?
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to purify ionizable molecules
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