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30 Cards in this Set
- Front
- Back
In the feline there are 2 types of retics, the punctate and the aggregate. Which of these types do you count? |
Aggregate |
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What is the purpose of performing a reticulocyte count? |
Access response of the bone marrow to the anemia |
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Which species of animal does not have circulating retics? |
Horse |
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How many retics are seen in this picture of dog blood? |
3 |
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What stain is used in preparing a reticulocyte count? |
New Methlyene Blue, NMB |
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What would a retic be called under diff-quik stain? |
Macrocytic polychromatic rbc |
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Which retic is considered the younger kind, aggregate or punctate? |
Aggregate |
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How many RBC's should be in a each field when looking for retics? |
100 RBC |
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What is the observed retic count? |
The observed retic count is the number of retics per 1000 RBC's |
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What is the formula for the corrected retic count? |
Patient PCV / normal PCV X observed retic |
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What is the formula for the absolute retic count? |
Take the observed retic (before you change it to a percentage) and multiply it times the total RBC count. |
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What is the procedure for retic staining, incubated/dry? |
Procedure: incubate equal parts blood/NMB at room temp for 15 min. Make thick push smear (since diluted and usually anemic). Air dry and evaluate on oil immersion. |
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Aggregate retics have less than 5 clumps of reticulum. True or False? |
False. Aggregate retics have greater than 5 clumps of reticulum. |
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What is the procedure for NMB wet-prep? |
Procedure: Take dry coverslip unstained smear and place bloodside down onto drop of NMB on slide. |
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Why is the incubated method the preferred method over the wet-prep method? |
Incubated method preferred since difficult to distinguish punctate from aggregate, and harder to read. |
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Punctuate retics have less than 5 clumps of reticulum. True or False? |
True |
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The wet prep method is used when? |
A limited amount of blood available. |
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What kind of retics are these? |
Aggregate |
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Your dog has a 15% PCV, and you counted 76 retics. What would be the corrected retic count for this animal? |
2.5% |
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What happens when retics are stained with NMB? |
When stained with NMB the ribosomes and other organelles of the immature RBC clump into visible granules. |
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Define a reticulocyte. |
A reticulocyte are immature erythrocytes that contain organelles that are lost as the cells mature. These organelles account for the diffuse blue-gray or polychromatophilic staining of the immature cells with Wright’s stain. |
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What is considered regenerative in regard to the absolute retic count? |
In regard to the absolute rectic count the normal dog and cat the absolute retic count is >60,000/µl. In the anemic animal one would like to see an absolute count of 100,000/µl or higher. |
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Define reticulocyte count. |
A reticulocyte count is an expression of the percentage of RBC's that are reticulocytes. |
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Feline reticulocytes come in 2 varieties: |
punctate and aggregate |
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Canine reticulocytes are almost always |
aggregate |
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The retic count is performed on oil but a suitable viewing area is found on high power.True or False? |
True |
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Would this be considered a proper area to count your retics? |
Yes |
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When counting retics, you view ________ number of fields with ___________ number of retics to get a total count of ________. |
10, 100, 1000 |
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In cases of severe blood loss anemia, how long might it take for retics to be seen in blood? |
2-4 days |
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Why might you not see retics in the blood until a few days after an anemic patient is admitted? |
The blood may not start regenerating immediately and may take a few days. |