Thin layer chromatography

Introduction: LC-MS is used for the bioanalysis of small molecules drugs, which has molecular mass of less than 800 Da. Now a days, it is also frequently used for the bioanalytical determination of biopharmaceuticals.1 In pharmaceutical R & D, it takes a year to develop a new marketing drug identified from thousands of new compounds. The drug discovery is divided into early and late phase, during which pharmacokinetics study or bioanalysis of a drug is done. Once, the bioanalysis of a drug is…

EXPERIMENTAL RESULTS The present study was undertaken to formulate Solifenacin succinate immediate release tablets. The study involves preformulation studies of drug excipients, formulation and processing development along with evaluation of tablets made with the optimized formulation. Finally tablets were evaluated by in-vitro methods. 6.1. Drug excipient compatibility study Table 1. Results of compatibility study S.No. Name of Excipient Ratio of API/ Excipient Initial…

ABSTRACT Flakiness & Elongation index is one of the most prominent criteria that govern behavior and performance of aggregate in the bituminous mixes. The strength and serviceability requirements of bituminous mixture such as Stability, Flow, Voids in Total mix (VTM), Voids Filled with Bitumen and (VFB) highly depend on the physical properties of aggregates. This study conducted by observing the effect of flakiness & elongated index by adding different percentages from 0% to 50% of flaky &…

Stereochemistry conformation is how molecules are orientated in space. There are two basic types of isomers: constitutional or stereoisomers. Constitutional isomers gave the same molecular formula but different connectivity, while stereoisomers have the same connectivity but a different orientation in space. Stereoisomer has two subunits called enantiomers and diastereomers. Enantiomers are molecules that are mirror images, while diastereomers are not mirror images. Stereochemistry focus…

Chromatography- This is a technique that is most commonly used to separate a solution into its different components, an extraction solvent is used to do this, the solution is added to the chromatography paper, and then as the extraction solvent is absorbed by the paper the different compounds with different weights. How does it work- Chromatography is used to separate a mixture into its components. It contains two key phases, a stationary phase that involves a sold or a liquid supported in a…

Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…

3.3.9. Encapsulation efficiency of drug loaded NPs The encapsulation efficiency (EE) was determined by weighing the amount of unloaded drug recovered from lyophilization of the supernatant obtained after centrifugation of NPs. The drug encapsulation efficiency was calculated according to the following equation: Encapsulation efficiency (%) = (Winitial/Wfree)/Winitial *100%. Where, Winitial and Wfree are the weight of initial drug and the weight of free drug determined in the supernatant after…

To assess the bioequivalence by comparing the single oral dose bioavailability of test product MYCOCEPT 500mg (containing Mycophenolate mofetil 500 mg) film coated tablets manufactured by (confidential) with that of reference product CELLCEPT 500mg (containining Mycophenolate mofetil 500 mg) film coated tablets distributed by Roche Registration Limited, shire park, welwyn Garden City, AL7 1TW, UK in healthy, adult, human subjects under fasting conditions. 4.2. STUDY DESIGN A randomized,…

Introduction Affinity Chromatography, also referred to as biospecific chromatography, is a technique of partitioning rooted in biological affinity (Dean). This practice capitalizes on complex interactions such as bond formation between enzymes and substrate, or more broadly: macromolecules and ligands. Purification is at the center of the use of affinity chromatography. In general, samples may be run through a gel that contains a corresponding component that binds with the sample at certain…

RESULT AND DISCUSSION 7.1. Preformulation studies 7.1.1. Identification of pure drug by FTIR Figure7.1: Identification of Sample through FTIR Figure 7.2: FTIR Spectrum of Standard Prazosin hydrochloride (with references of B.P.- 2009) Table: 7.1 - Characteristics peaks of Prazosin hydrochloride S.No. Reference peaks (cm-1) Theoretical peak of Prazosin hydrochloride Obtained peaks (cm-1) Functional Group Stretching/ Bending 1 1750-1680 1529.92 1529.92 C=O Stretching 2. 1650-1580…