Paper chromatography

ABSTRACT Flakiness & Elongation index is one of the most prominent criteria that govern behavior and performance of aggregate in the bituminous mixes. The strength and serviceability requirements of bituminous mixture such as Stability, Flow, Voids in Total mix (VTM), Voids Filled with Bitumen and (VFB) highly depend on the physical properties of aggregates. This study conducted by observing the effect of flakiness & elongated index by adding different percentages from 0% to 50% of flaky &…

Stereochemistry conformation is how molecules are orientated in space. There are two basic types of isomers: constitutional or stereoisomers. Constitutional isomers gave the same molecular formula but different connectivity, while stereoisomers have the same connectivity but a different orientation in space. Stereoisomer has two subunits called enantiomers and diastereomers. Enantiomers are molecules that are mirror images, while diastereomers are not mirror images. Stereochemistry focus…

Introduction Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…

the alleged sticky bandit. The note was sent to the Tempe CSI Crime Lab for further analysis. Blaine Benson, head of the Tempe Crime Lab, has reported that paper chromatography will be used to identify the pen the bandit used. Pens from local syrup competitors were collected as evidence to compare to the ink used in the note. Paper chromatography is a common practice used to separate the colored components in the ink. The Rf value or the retention factor is the ratio of the distance traveled by…

The idea of music playback came from Édouard-Léon Scott de Martinville, a bookseller, and trader from Paris. In 1857, Scott obtained a fascination for the human ear which led him to invent the Phonautograph, an instrument primarily used to study acoustics in laboratory research. The construction of the phonograph was heavily inspired by the anatomy of the human ear. Consisting of three main components that replicated the build of the ear canal, eardrum, and ossicles. Sound waves would be…

The purpose of this experiment was to identify the generic name of the unknown drug through thin-layer chromatography analysis. By comparing the Rf values obtained from each TLC plate, it was deduced which components were present in the unknown drug. Through comparison of Table 1 and Table 2, Unknown A was identified as B.C. Tablets. Salicylamide was evident in the unknown substance due to the distinct blue color that was present in both the unknown spot and the salicylamide spot; the similar Rf…

3.3.9. Encapsulation efficiency of drug loaded NPs The encapsulation efficiency (EE) was determined by weighing the amount of unloaded drug recovered from lyophilization of the supernatant obtained after centrifugation of NPs. The drug encapsulation efficiency was calculated according to the following equation: Encapsulation efficiency (%) = (Winitial/Wfree)/Winitial *100%. Where, Winitial and Wfree are the weight of initial drug and the weight of free drug determined in the supernatant after…

To assess the bioequivalence by comparing the single oral dose bioavailability of test product MYCOCEPT 500mg (containing Mycophenolate mofetil 500 mg) film coated tablets manufactured by (confidential) with that of reference product CELLCEPT 500mg (containining Mycophenolate mofetil 500 mg) film coated tablets distributed by Roche Registration Limited, shire park, welwyn Garden City, AL7 1TW, UK in healthy, adult, human subjects under fasting conditions. 4.2. STUDY DESIGN A randomized,…

RESULT AND DISCUSSION 7.1. Preformulation studies 7.1.1. Identification of pure drug by FTIR Figure7.1: Identification of Sample through FTIR Figure 7.2: FTIR Spectrum of Standard Prazosin hydrochloride (with references of B.P.- 2009) Table: 7.1 - Characteristics peaks of Prazosin hydrochloride S.No. Reference peaks (cm-1) Theoretical peak of Prazosin hydrochloride Obtained peaks (cm-1) Functional Group Stretching/ Bending 1 1750-1680 1529.92 1529.92 C=O Stretching 2. 1650-1580…

1. Abstract In this study, reverse micellar extraction of asparaginase model system was carried out using CTAB/Iso-octane/hexanol/butanol system to optimize the forward and back extraction efficiency. By using Taguchi’s orthogonal array (OA), maximum forward extraction efficiency of 55.0%, 61.0% and 54% was achieved with an aqueous phase pH of 11.0, 150mM CTAB/Iso-octane and 0.1 M NaCl, respectively. Similarly, Taguchi’s orthogonal array (OA) was applied to optimize the pH of stripping phase,…