Aaminah Azhar Astrobiology Spring 2015 Test 1 1. Name and describe five important steps in the formation of planets based on our current scientific understanding. a) Star and planet formation starts inside a collapsing cloud of gas and dust inside a bigger cloud called a nebula. Because of gravity pulling materials in the collapsing cloud closer together, the center of the cloud gets increasingly more compressed and because of this get hotter. The dense hot core of this collapsing cloud becomes…
Planetary, supernova, dark, and reflective are all types of nebulae that are formed and act differently. The elements found in the planetary nebulae and supernovas. A scientist has found that in the permitted lines of planetary nebulae there are nitrogen ions (Aller). Permitted lines are only found with a high power telescope; this shows that there are more possibilities of other elements within these clouds. The elements that are mostly know in Planetary nebulae are the common “hydrogen,…
First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…
The purpose of this experiment is to understand how to culture bacteria, conduct a Gram stain on a bacterial culture, and properly carry out a Kirby-Bauer assay to test the efficacy of different antibiotics. The Kirby-Bauer assay was developed in 1956 by W.M. Kirby and A.W. Bauer at the University of Washington School of Medicine (Hudzicki 2009). The general idea behind this procedure was to test the susceptibility of bacteria to certain antibiotics. If a disc containing an antibiotic is placed…
Macbeth, a story written by Shakespeare, concludes a story in which has content that is shown effectively and expressed in a couple different ways. The films that were shown such as Roman Polanski, Philip Casson and Rupert Goold had their own techniques of interpreting Macbeth. In this instance, for Act 1 Scene 1 when looking at the witches, all three film clips are focused on the shots to emphasize the witches. For Act 2 Scene 2 when the clips are showing the post murder meeting of Macbeth and…
Media is a dynamic institution that reflects the needs, wants, and desires of society. It is constantly developing in rhythm with humans over time to cater to our informative needs. The creation of the internet, then cellphones, and social media portrays the rapid growth and changes of the institution. The 21st century is rapidly advancing and therefore, we cannot guarantee its future but we can predict it. The theories of the ‘hot and cool media’, ‘the global village’, and the ‘narcissism…
When it derives to contemplating out an unknown bacterial species the whole purpose is to benefit us in order to better understand what this species is capable of. Throughout a general basis of identifying an unknown species we must figure out either it will become a gram-positive or gram-negative type of bacteria and in doing so we must do a gram staining method. This method works because it will direct us to what test we can do next based off either it is negative or positive bacteria, which…
2.5. ANTIOXIDANT STATUS 2.5.1. TLC - Semi Quantitative DPPH Assay 0.2 % DPPH solution in methanol was prepared and kept in the fridge for further use. The grid space was marked with 1.0 cm2 space on an aluminum based TLC sheet (Merck silica gel 60F254) and a stock solution of all the extracts together with the standard were prepared in methanol. A series of dilutions of the stock together with the standard were prepared ranging from 400 µL to 0.01µL for the last dilution. The grid on the TLC…
Blood Culture Contamination For the purpose of this research, Archbold Medical Center Laboratory is the place I chose to conduct my research. Blood Culture contamination is a big problem not only at Archbold, but at many hospitals. The problem is not only within the laboratory, but from outside sources also. Although contamination is a problem, it can be easily fixed. Whenever a blood culture is ordered by a physician, we can assume the patient is experiencing some type of bacterial infection…
To begin, a streak was performed on two TSA plates and a blood agar plate using the three-sector streak technique from the unknown culture tube to isolate the two unknown bacterial colonies. The plates were then incubated for 12 hours at 37 ̊C. The blood agar plate was used to create a greater contrast in color to help distinguish between bacterial colonies by size and color. Two distinct colonies could be seen growing on the blood agar plate shown in figure 1. The smaller, opaque colored…