High performance liquid chromatography

High Performance Liquid Chromatography Lab Abstract High performance liquid chromatography or HPLC is the process in which mixtures are physically separated according to their polarity. The lab conducted, the separation of dyes in grape Kool-Aid, demonstrated the concept. This was done to display how different alcohols and their concentrations have different polarities, in which ties into HPLC. The lab consists of testing Kool-Aid, water, and different concentrations of methanol and isopropanol; 5%, 20%, and 60%. To start, one must pour 10 ml of the Kool-Aid into a syringe that was connected to a C-18 absorbent. The plunger that came with the syringe will then be pushed through into a labeled test tube designated for the substance. Once…

Raju Chandra et al; A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of quetiapine fumarate from marketed bulk tablets. The active ingredient of quetiapine fumarate separation achieved with C18 column using the methanol water mobile phase in the ratio of 30:70 (v/v). The active ingredient of the drug content quantify with UV detector at 359 nm. The retention time of quetiapine fumarate is 5.27 min. A good…

1.5 ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY (UPLC) Since from thirty years, High performance liquid Chromatography (HPLC) is well accepted technique used in laboratories worldwide. It is one of the analytical techniques used to identify, separate and quantify each analyte in a mixture. One of the primary drivers for the development of this technique has been the progression of the packing materials (particle size, packing technology, chemistry) used to impact the separation. The fundamental…

Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…

High performance liquid chromatography (HPLC) is a separation technique utilizing differences in distribution of compounds in two phases; called the stationary phase and the mobile phase. The stationary phase designates a thin layer created on the surface of fine particles and the mobile phase designates the liquid flowing over the particles. Under a certain dynamic condition, each component in a sample has a different distribution equilibrium depending upon the solubility in the phases and or…

Observe Ligand-Protein Interaction using Gel Filtration Chromatography (Phenol Red Binding to Bovine Serum Albumin) Introduction: Serum albumin is often known as blood albumin, and is the most abundant protein in humans and other animal plasma. Serum albumin is also known as a carrier protein by non-specifically binding several biomolecules. It is essential for maintaining the osmotic pressure that is needed for body fluids distribution between body tissues and intravascular compartments.…

Adsorption is the bond of particles, particles, or atoms from a gas, fluid, broken down and attached strongly to a surface. This procedure makes a film of the adsorbate on the surface of the adsorbent. This methodology varies from absorbtion, in which a liquid (the absorbate) pervades or is absorbed by a porous solid. Adsorption is a surface-based procedure while absorption includes the entire volume of the material. The term sorption envelops both methodologies, while desorption is the converse…

Introduction: Acetaminophen is a common pain reliever and fever reducer used to treat conditions such as head, muscle or tooth ache, as well as colds, fevers, and even arthritis. It is available in a tablet form to be ingested. These tablets are manufactured by a large variety of pharmaceutical manufactures including a great deal of generic brand companies. These tablets all have the same active ingredient, acetaminophen, yet each hold different inactive ingredients that can impact the overall…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

rate was much higher than that in seawater. As seen in Fig.5, the polished wear scars were obtained on the wear tracks. While the scratches appeared on the wear scar of mating balls in seawater, especially, white scratches presented on the age of wear scar in Fig.5j. That result was attributed to the calcium magnesium salt precipitation (Mg(OH)2, CaCO3), which played the role of abrasive particles on the friction interface and resulted in scratches on the wear scar of balls. On the other hand,…