Hemolysis

After being incubated in a water bath at 37 degrees Celsius for 20 minutes, the 8 samples were centrifuged at 400 rpm for 5 minutes and collected for observations. Presence of a red pellet in the first 7 tubes, including control Test Tube 1 were noted, while the red pellet was absent in control Test tube 8. It must also be noted that the pellet became progressively smaller as each successive tube was examined, a result more noticeable after examination of Test tube 5, as seen in Figure 1. The transparency of the solution changed in tandem with the colour of the solution. While Test tube 1 returned a clear and colorless solution, as successive tubes were examined the samples became more translucent and a red tint appeared by Test tube 4, growing more bold in colour with progressive Test tubes. This resulted in the final sample (Test tube 8) being a red translucent solution lacking the red pellet as shown in Figure 1. The incubator maintains a constant temperature of 37 degree Celsius to mimic the internal environment of the human body, which furnished the homes of various pathogens common to the human body. The red blood cells are incubated with the antibody under these favorable conditions so that the antibodies can initiate the cleavage of the C5 protein, allowing the membrane attack complex to form a pore on the sheep erythrocyte surface. Upon formation of this attack complex the cell present in the sample will begin to lyse, the amount of cell lysis dependent on the…

Footstrike Hemolysis Footstrike hemolysis happens when red blood cells in the foot break down faster than usual. This is usually due to repeated contact between the foot and the ground. This condition often happens to long-distance runners. It can also happen to other athletes, including dancers and hikers. This condition may also be called march hemoglobinuria. This condition may cause part of the red blood cells (hemoglobin) to appear in the urine, making the urine red (hematuria). In most…

we aim to that % hemolysis is going to decrease as osmolality increases because the more concentrate is the solution, the less cells going through hemolysis. Thus, there is not enough water entering the cell to produces the cells to burst. The main clue is when the water is completely dilute, so we have 100% hemolysis. Hemolysis is correlated with the behavior of the cell specifically refers as hypotonic. The results correlated with the raised hypothesis, the data overall represents the…

species through a series of morphological and biochemical tests. The tests ran in this experiment, in order, was a Gram-stain, a Catalase test, and Red Blood Cell hemolysis (RBC hemolysis) on a blood agar plate. To determine the unknown species these tests were ran in order, first to determine the morphology of the species by using Gram-staining. The Gram-stain resulted in a positive test with a cocci shape. Next a Catalase test was used to determine if the species used catalytic enzymes. The…

system that consists of a group of proteins that work together to destroy foreign invaders such as viruses and bacteria. These proteins normally protect red blood cells from destruction by the complement. People with PNH are missing two important complement-regulating proteins. Due to this missing protein, the red blood cells are prematurely destroyed, which leads to hemolytic anemia. Hemolysis by the complement system leads to episodes of hemoglobin in the urine, which would then be…

(normal = 120 days) or premature destruction of red cells. * Elevated erythropoietin levels and increased erythropoiesis in the marrow and other sites (extra medullary hematopoeisis), to compensate for the loss of red cells. *Accumulation of the products of hemoglobin catabolism from red cell destruction. Classification Of Hemolytic Anemias is based on: 1. The site / place of hemolysis i.e.wheteher it is intravascular or extravascular 2. Whether the underlying cause of red cell…

From the second part of the example, cell burst is a possible situation can occur to the cell. In this experiment, cell burst, more specifically hemolysis, red blood cell death, lead organelles to spread in the solution and block the absorbance of light in the colorimeter. By comparison of the measured osmolarity and hemolysis between the different solutions provided the data to understand, how a red blood cell react in different osmolarity to maintain…

consistent with severe anemia including of pallor, fatigue, tachycardia, tachypnea, and hypotension. The patient’s new systolic ejection murmur is most likely related to increased semilunar flow due to the anemia. The recent throat infection and subsequent antibiotic treatment are the most probable cause of these symptoms. The patient’s leukopenia is also most likely a transient response to his infection, with another possibility being a drug reaction to treatment that he received for his…

Patients with PNH often present with hemoglobinuria, abdominal pain, fever, headache and fatigue. While hepatic and cerebral veins are the most common sites of thrombosis, PVT is extremely rare with only about 12 cases reported in literature. Sparing of hepatic vein with involvement of portal vein and mesenteric veins makes this case highly unusual. A gastroenterologist must be keenly aware and consider the diagnosis of PNH in any young patient presenting with abdominal pain and hemolysis with…

coagulase positive; otherwise the microbe is coagulase negative. The catalase test differentiates between microbes than can produce the enzyme catalase or not. If a microbe has the catalase enzyme, when hydrogen peroxide is added, bubbles appear making it catalase positive. If no gas or bubbles are observed, the microbe is catalase negative. A differential agar used for this experiment was the Blood agar. This agar differentiates between microbes that can create exotoxins to lyse the red blood…