To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because…
Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…
In this experiment Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was performed under reducing conditions to estimate the molecular weight of proteins within a sample. The protein sample was from the previous using anionic exchange chromatography to partially purify N-acetyl--D-hexosaminidase, Lab 3. The molecular weight was determined through the interpolation of a standard curve of proteins with a known molecular weight. The molecular weight of band 1 was determined to…
Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard. Methodology: The experiment begins with the denaturing of the proteins through heating by…
was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small molecules is greater than that of large…
“Music speaks what cannot be expressed, soothes the mind and gives it rest, heals the heart and makes it whole, flow from heaven to the soul (Monet).” Feeling good was originally written in 1965, by Nina Simone. This song has been used in the popular culture such as, “Point of No Return” and “Six Feet Under”. A song cover was later done by Jennifer Hudson in 2011, and Lauryn Hill in 2015. Although Nina Simone wrote the song, Jennifer Hudson sang it better due to melody, rhythm, and…
Cleaning Your Hair Your hair washing schedule should be based on how much oil is in your hair. If you have very oil scalp you will want to wash your hair daily. If your hair is chemically treated your hair will be drier and you won’t want to wash it too often. As we age our scalps produce less oil and so the older your get the less frequently you will need to wash your hair. If you find flakes on your scalp then you will need to shampoo more often than you are currently doing. The right shampoo…
The particular sites of the human body that are considered to be problematic by women according to Rice are the skin, weight, hair, and the breasts. The author goes ahead and makes it clear that the debate of obesity within the North Americas is usually framed and shaped by human weight and dieting. According to her argument, the people in North America have for long been blamed…
influence. As time goes on the trends seem to get more extreme and impractical such as women shaving all their unwanted hair, where men are encouraged to not shave theirs. Everywhere a woman looks there is a reminder or advertisement for hair removal and all the different options for it. The United States has only had a few occasions when women speak out against not shaving. Hair removal is a vital contributor for women to be seen as beautiful. Today’s society has an unrealistic expectation for…
and handicapping her for eternity. These heroic metaphors create just a dent in Alexander Pope’s satirical epic The Rape of the Lock. In reality, the woman’s armor is her makeup, the guardians are just a group of ineffectual sprites, and a lock of hair is stolen from the hero’s head, causing a trifling temper tantrum. The Rape of the Lock can be compared to Beowulf in that it follows the guidelines of a heroic epic. However, The Rape of the Lock transforms the ideas of heroism by heavily…