Ethylenediamine

Introduction What are enzymes and how do they affect reactions? Enzymes force reactions, in cells, to maintain a speed necessary for life. Enzymes can also be considered catalysts because they require the reactions to arise faster without themselves changing. For this experiment, the substrate in the enzymatic reaction was sucrase, and it will be causing sucrose to change resulting in the following equation: Sucrose → Glucose + Fructose Where the arrow is in the equation indicates the enzyme, sucrase, used to speed up the reaction. In the experiment conducted we were looking for the effect of a cofactor that could have impacted enzyme activity. A cofactor is required for enzymes to function properly. In order to catch sight of the reaction occurring, we must use catechol, because it is colorless and then observe it being catalyzed changing it to benzoquinone. Benzoquinone is reddish-brown so the reaction of catechol to benzoquinone can be easily seen, however for it to occur you must have catacholase. Catacholase is the catalyst for the conversion of catechol to benzoquinone. The equation for catechol to benzoquinone is: Catechol + O2 → Benzoquinone + H20 Where the arrow is, for this experiment, is catacholase. However, this reaction occurs countless times throughout life without that enzyme actually being present. For example, when an apple turns brown after being bitten, is an example of this reaction. The variable that was being tested in this experiment was the…

Therefore, when H1- antihistamines binds to H1R it opposes the effect of histamine preventing or decreases the severity of an allergic reaction. There are different generations of antihistamines. The first-generation antihistamines are the oldest drugs whereas the second and third generation are the newer drugs. The main difference between the old and new generations of drugs is that the first-generation are highly lipophilic and less selective resulting in them entering the CNS resulting in…

Organic ingredients such as para-aminobenzoates, cinnamates, benzophenones, dibenzoylmethanes, camphor derivatives and benzimidazoles, absorb the ultraviolet radiations. Oxybenzone is a synthetic estrogen that disrupts the hormonal system when it deeply penetrates the skin. Inorganic ultraviolet chemical filters such as zinc oxide and titanium oxide reflect and scatter the ultraviolet radiation to protect skin. Other ingredients include preservatives (e.g. parabens derivatives), coloring agents…

Shoot length, root length, fresh weight and dry weight (80ºC for 24 h until a constant weight) were measured. 1 gm leaf sample was taken and chlorophyll a+b, carotenoids were measured (Aron, 1949). For biochemical analysis, 1 gm ground leaf sample was homogenized in 2 ml of 0.1 M ice cooled phosphate buffer containing 0.1 mM ethylenediamine tetra acetic acid and 1% (w/v) polyvinylpyrrolidone and inoculated 30 min in ice. This homogenate sample was centrifuged at 10000 rpm for 30 min at 4ºC and…

to low λmax), which is the same as sorting from weak field to strong field, the ligands examined in this experiment are CO3, ox2-, gly, NH3, NO2, and H2O. This spectrochemical series differs from the spectrochemical series presented in the provided proedure1 in two manners. First, CO3 and gly are not present in the presented series. Second, H2O was found to be more strong-field than the other ligands in this experiment, whereas the presented series gave H2O as a weaker field than NH3 and NO2.…

concentration of NO3- in rainwater is decreasing at Mount Rainier National Park because of the Clean Air Act that was previously passed to reduce atmospheric pollution. The last hypothesis is that that the rainwater concentrations of NO3- at Mt. Rainier N.P. will correlate inversely with rainwater pH and directly with [H+] because nitrate causes rainwater acidification. Experimental Description: In this experiment an autoanalyzer from Lachat Instruments was used to calculate the nitrate levels.…

al. prepared new aminocellulose derivatives by nucleophilic substitution reaction with ethylenediamine (EDA) and selected oligoamines starting from 6(2)-O-tosylcellulose (DSTos= 0.8 that reacted with (EDA), (DETA), (DPTA), (TETA). All the synthesized aminocellulose tosylates were soluble in water, and their transparent films formed complexes with Cu2+ ions, and used to immobilize glucose oxidase enzyme86, scheme 34. Liu et al. studied the effect of temperature and solvent on the nucleophilic…

orange light the best and absorbs green light the best. With this information, we determined that to measure enzymatic activity, we could measure the change in absorbance of green light in benzoquinone. A spectrophotometer was used to do so, and was set to measure absorbance which has no units, rather than transmittance. A major part of enzymatic reactions is the need for cofactors. Cofactors allow enzymes to form the proper shape so that the enzyme’s active site will bind to the substrate and,…