Capillary electrophoresis

IONIC LIQUIDS Introduction:- Ionic liquids are actually salts that are liquid in state, normally referred to compounds that have melting point below an arbitrary temperature i.e. 100° C. Unlike some other ordinary liquids like water and gasoline, that are composed of neutral species while ionic liquids are composed of ionic species and ionic intermediates commonly known as “ionic fluids” and “liquid electrolytes”. In ionic liquids there is a species that has a delocalized species and the other…

thickness. The coated plates were activated by incubating at 100ºC for 3h prior to use. The sample and positive control (Vinblastine, Sigma, 1mg/ml) were spotted on to activated TLC plate just 1cm above the subordinate edge of plate with the help of capillary tube and allowed to air dry. Simultaneously, the TLC chamber was saturated with different solvent systems (Binary and tertiary) consisting of mixture of solvents of different polarities and ratios for 20 min. The TLC plate was kept in…

physical properties such as polymerization, contrasting solubility and molecular stability. This leads to the erythrocytes becoming sickle-shaped and not able to bind oxygen efficiently. Sickled red blood cells can clog small blood vessels and capillaries, resulting in a lack of oxygen for the tissues. That can cause chronic pain, tissue infections and damage of organs. Protein…

Introduction Sickle cell disease is a genetic disorder that can be best described as irregular shaped red blood cells that block blood flow, which cause chronic periodic episodes of pain. Sickle Cells affects over 72,000 Americans and millions throughout the world, Sickle cell most commonly affect African American descent, approximately 1 in 12 African Americans carry the trait for Sickle cell and 1 of every 350 African-American infants born have the disorder and the incidence of the disorder…

The famous discussion of nature vs. nurture was somewhat interrupted by the realization that both aspects, genetics and environment, have important input in individuals. By combining the studies of genetics and psychology it is possible to correlate results from the fields and search for possible, and hopefully specific, genes involved in the expression of certain traits. For instance, Poulin et al (2011) explored the relationship between receptor genes of oxytocin and vasopressin and prediction…

or other ethnic groups found in the Middle East and in countries along the Mediterranean Sea. In comparison to the red blood cells, sickle cells are stiff and less flexible, making them unable to flow freely through veins and arteries and narrow capillary passages (Kayser et al., 2013). They thus create a blockage between the blood vessels and as a result less blood and oxygen is transported…

treatment of hypertension. It has a more potent active metabolite (II, 2-n-butyl-4-chloro-1-[2- (1H-tetrazol-5 yl) biphenyl- 4-yl) methyl] imidazole-5- carboxyl acid)[1]. The determination of Losartan has been carried out in tablets by HPLC, capillary electrophoresis and super-critical fluid chromatography[2,3], in urine by gas chromatography- mass spectrometry[4] and, simultaneously with its active metabolite in biological fluids, by HPLC[5- 10]. Amlodipine Besylate (ADB) )[FIG.1.B],…

Intro The technique of DNA profiling was first discovered in 1985 by Sir Alec Jefferys, a Leicester University geneticist. The work that he did in the laboratory led to the discovery of the technique for genetic profiling, which revealed a pioneer method of biological identification. A humans DNA can be found in hair, saliva, blood, and other various human properties. Sir Alec Jeffreys knew that what he had done was going to be revolutionary. After his discovery, he said “My life changed on…

Pollution is one of the most serious problems that affect our environment, and its effects on all life aspects are clear to everyone. Because of this, pollution is one of the most serious issues that all the word countries are concerned about. As time goes, the pollution problem increases and also the population growth increase. The increase in demand for water and food supplies place an increasing stress on the ground and surface water quality and quantity. One of the environmental problems…

were labeled Mix, Phe, Lys, and Ala (Figure 1). On the second plate, four marks were drawn, labelled as Mix, pH3, pH6, and pH11 (Figure 2) and this plate was then placed aside for use later in the experiment. The first plate was then spotted using capillary tubes and spotting a tiny drop of each amino acid on its labelled spot, then using the mixture to also make a spot on its mark. This plate was then sealed inside the developing jar and allowed to run until the solvent reached about ¾ of the…