Capillary electrophoresis

DNA fingerprinting, also known as DNA profiling, is identifying an individual using there DNA. This is often used for identifying criminals, and parental testing. First, a sample must be provided, which can be: blood, semen, hair roots, or saliva. The cells from the sample are then split open, and DNA is separated from the rest of the cell. The DNA is then treated with specialized proteins (restriction enzymes),which separate the DNA into smaller parts. The fragments of DNA are then applied to a…

Agarose gel electrophoresis of DNA is used to seperate mixtures of protein and nucleic acid fragments. Molecules tend to differ in size, charge, and mobility. The objective of this procedure is to predict the distance of DNA migrating towards the positive electrode in the electrophoresis chamber. Many scientists use agarose gel electrophoresis to examine the DNA of criminal suspects. The first step in this procedure is to prepare a buffer containing 1% of the liquid agarose gel. Once the gel…

have retained its shape or charge. This would result in an entirely different band than the rest of the protein that properly bound to SDS. If the unknown protein were migrating with the dye front, it would require constant monitoring during electrophoresis in order to measure the molecular weight. If the gel were constantly monitored and stopped immediately when all of the proteins were separated out (probably when the dye front hits the bottom of the gel, but potentially sooner), it…

The samples were placed for five minutes in a 95 C heating block. After the instructor loaded the standard into the gel, members loaded their labeled protein samples into the appropriate electrophoresis wells. The cover of the apparatus was attached and the associated colored cables. The apparatus was slowly turned on to a voltage of 90 V and allowed to run. The standard loaded by the instructor was monitored as it was dyed and was used to measure…

the transformation of the ade2 gene to the kanamycin resistant gene (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3.…

cell lysis buffer (). Next, the proteins from the healthy volunteers and the sick volunteers will be stained with CyDye Cy3 and Cy5, respectively, and combined into one solution. The resulting solution will then be subjected to 2-dimensional gel electrophoresis. This will be done by transferring the mixed protein solution to isoelectric tube gel (pH 3-10) and subjecting it to a constant electric current (145mA). After the gel has been allowed to run for about an hour, the isoelectric tube will…

Denaturing Protein Gels Kaytlin Goodwin Cedarville University Introduction Electrophoresis experiments are conducted on proteins for the purpose of separating proteins based on their characteristics. The characteristics that can be examined during the experiment are dependent on the preparation of both the proteins and the gel medium through which they will run. Native gel electrophoresis experiments highlight and examine multiple protein characteristics, such as size, charge, and…

Sweta Soni 2/22/15 Biochem Lab Experiment 5 Introduction The purpose of Experiment 5-1 is to separate a mixture of three molecules—blue dextran, cytochrome c, and potassium chromate—through the use of gel-filtration chromatography. The volume at which each molecule elutes will be recorded in order to determine the accuracy of separation. The purpose of Experiment 5-2 is to purify the E. coli enzyme, β-Galactosidase through the use of affinity chromatography on…

vaccines, such as the influenza or polio vaccines are examined, purified and improved, gel electrophoresis. Due to confidential reason, the exact process of vaccines analysis are unknown, however it is suspected that the steps are similar to DNA analysis by electrophoresis and medical reports have claimed that electrophoresis presents an effective method of vaccine analysis (ehow). Through gel electrophoresis, scientists are able to identify the types of bacteria that are antibiotic resistant…

reason could be that the cell did not get enough heat shock, or not enough pre-incubation time with CaCl2, which means the bacterial cell wall was not permeable enough for the DNA to come inside and interact with the bacterial DNA. The DNA gel electrophoresis showed that there were no fragments…