Bradford protein assay

After transcribing and translating the sequence I found out that the protein from my DNA sequence is Dystrophin. Dystrophin is known as the DMD gene or Duchene Muscular Dystrophy. (“DMD”, 2015)This gene is one of the largest known human genes in the body. Dystrophin is used for movement such as your muscles and your heart. Dystrophin also plays a role in cell signaling. This sends and receives protein chemical signals. (“DMD”, 2015) Dystrophin is not only important for the structure, but the…

Proteins are complex molecules that serve a variety of functions within the body. Proteins are made up of subunits called amino acids. There are several different types of amino acids such as lysine, aspartic acid, and histidine. Each type of amino acids have their own specific group called R-groups attached to them, this causes them to behave chemically different. The group on the amino acid histidine cause the amino acid to be basic; this feature of histidine makes it have a specific purpose…

Finger nails are made up of keratin protein (the same keratin found in hair), which is formed by amino acids, and produced by living skin cells. The main components that make up a nail consists of; the nail plate which is located at the top of the nail. It is held together by protein fibers. The nail plate needs blood flow, oxygenation, and nutrients to grow. This is why an injured nail sometimes turns bruised or falls off. Oxygen penetrates through the nail plate. The nail bed is the skin…

Recent technological advances in biology have prompted me to want to study the subject as an undergraduate. I have been drawn to how the world works and what everything is made up of since secondary school, but in recent months my focus has shifted specifically towards the study of genetics. A wide and varied subject, ranging from eugenics and the ethical considerations of genetically engineering organisms, and to the more recent scientific breakthroughs in said area of study, the study of…

This means finding proteins that express affinity and specificity for the respective ligand. According to Ewald (2011) there are three good options, CitX (periplasmic binding protein from Salmonella typhimurium) and the binding domains of DpiB (sensor histidine kinase from E. coli) and CitA (sensor histidine kinase from Klebsiella pneumoniae). We would then purify all three proteins and record fluorescence energy values when bound to citrate for future…

The purpose of this experiment is to examine the extraction of the DNA material that was extracted from the Chromosomal DNA from E coli in laboratory 9. DNase is used to help extract the Sugar phosphate backbone of the DNA. DNase should only cleave DNA not RNA but DNase can classify contaminated RNA from chromosomal DNA. Methods A. preparation :E. coli was used to extract the DNA as instructed in laboratory 9 to use in the experiment on analyzing in this experiment. There was 500 μL of the…

The simple definition of Eukaryotes is that they are any organism whose cells contain a nucleus and other membrane enclosed organelles. Taxonomically, Eukaryotes belong to the taxon Eukarya. The three types of eukaryotic microbes are fungi, protozoa, and algae. Because the three types of eukaryotic microbes are all composed of eukaryotic cells, they have basic similarities in cellular structure, including the presence of a nucleus. However, these types of microbes do differ in many significant…

According to T.F. Gendron et al., partial amounts of the toxicity are due to a gain in function by the protein. When this occurs, the TDP-43 protein has usually shifted its location from the nucleus to throughout the cytoplasm of the cell. This abnormal accumulation of TDP-43 in the cytoplasm is not only found in neurons and glial cells of the primary motor cortex but as well as in brainstem motor nuclei, the spinal cord, and in certain associated white matter tracts (Mackenzie et al 2010). It…

α-Lactalbumin, a multifunction protein in milk, forms an enzyme complex with galactosyltransferase that catalyzes the last reaction in lactose synthesis.1 α-Lactalbumin has one Ca2+ binding site and several Zn2+ binding sites as well as many intermediary folding states, making it ideal for research involving the effects of cation binding on protein interactions as well as research on protein misfolding.1 α-Lactalbumin gives milk an antimicrobial property, making it important for infant health,…

proteins like NSF, SNAPs (soluble NSF attachment proteins), and SNAREs including SNAP25, VAMP and syntaxin. With these identified proteins, Rothman laboratory developed the SNARE hypothesis describing a model of SNAREs involvement in vesicle targeting and fusion. Their model explains that a transport vesicle (v-SNARE) and a target membrane (t-SNARE) with the aid of NSF, SNAPs and other yet unknown proteins bring the v-SNARE in close proximity to the t-SNARE facilitating membrane fusion (Sollner…